Project Number: 5358-22000-033-03
Project Type: Grant

Start Date: Jul 31, 2006
End Date: Sep 30, 2009

Objective:
Develop a broad spectrum PCR-based method for the detection of a wide range of DMV isolates. Produce antisera to selected DMV proteins and their use in ELISA-based virus detection assay. Evaluate and develop a reliable protocol for meristem tip culture for producing virus-free material.

Approach:
Based on sequence comparisons of several U.S. isolates and European isolates, PCR primers will be designed that would represent the conserved regions and will be tested for their ability to detect a wide range of DMV isolates. Proteins will be gel-purified and used for immunization. Sera will be collected and tested for their specificity to detect DMV in infected dahlia plants. Using standard tissue culture protocols, meristem culture will be evaluated for its effectiveness in eliminating DMV from cultures that are already infected. Documents Grant with WSU. Formerely 5358-22000-028-05G (8/2007).