U.S. Geological Survey Toxic Substances Hydrology Program--Proceedings
of the Technical Meeting, Colorado Springs, Colorado, September 20-24, 1993,
Water-Resources Investigations Report 94-4015
Anaerobic Growth of Bacterial Strain Ses3 with Selenate as the
Electron Acceptor
by
Jodi Switzer Blum (U.S. Geological Survey, Menlo Park, Calif.),
Charles W. Culbertson (U.S. Geological Survey, Menlo Park, Calif.), and
Ronald S. Oremland (U.S. Geological Survey, Menlo Park, Calif.)
Abstract
The bacterial strain SES3 is capable of growth
with selenate, nitrate, or Fe(III) as electron
acceptors, and lactate as an electron donor.
Selenate is reduced through selenite to elemental
selenium, whereas nitrate was reduced through
nitrite to ammonia, and Fe(III) was reduced to
Fe(II). Lactate was oxidized to CO2 and
acetate in an approximately 1:1 ratio. Results
of washed-cell experiments comparing nitrate
and selenate reduction in either nitrate-grown or
selenate-grown cells indicated that separate enzyme
systems performed the reductions. Washcloth experiments
using uniformly labelled, [U-l4C] lactate,
showed that fumarate, arsenate, and O2 also functioned
as electron acceptors. The respiratory inhibitors
azide, carbonyl cyanide m-chlorophenyl-hydrazone (CCCP),
and dinitrophenol (DNP) inhibited both selenate
and nitrate reduction in washed-cell suspensions.
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