Projects

INTEGRATED BIOSENSOR-BASED PROCESSES FOR MULTIPATHOGENIC ANALYTE DETECTION Appropriated (D)   Accession Number: 410669

IMPROVED DETECTION TECHNIQUES FOR FOODBORNE PATHOGENS Appropriated (D)   Accession Number: 412743

IMPROVED DETECTION TECHNIQUES FOR FOODBORNE PATHOGENS Grant (G)   Accession Number: 412854

Publications

Applications of Immunomagnetic Beads and Biosensors for Pathogen Detection in Produce - (Book/Chapter) - (15-Apr-08)

Applications of Immunomagnetic Capture and Time-Resolved Fluorescence Detection for Salmonella Enteritidis in Liquid Eggs - (Proceedings/Symposium)

Tu, S., Gehring, A.G., Paoli, G. 2008. Applications of Immunomagnetic Capture and Time-Resolved Fluorescence Detection for Salmonella Enteritidis in Liquid Eggs. International Society of Optical Engineering (SPIE).6983:1-5 . Optical Engineering.

Detection of Salmonella Enteriditis from Egg Components Using Different Immunomagnetic Beads and Time-resolved Fluorescence - (Peer Reviewed Journal)

Tu, S., Reed, S.A., Gehring, A.G., He, Y. 2008. Detection of Salmonella Enteriditis from Egg Components Using Different Immunomagnetic Beads and Time-resolved Fluorescence. Journal of Food Analytical Methods. Available:http://www.springerlink.com/content/68381p3740355j10/

Detection of Salmonella Enteriditis from egg components using different immunomagnetic beads and time¿resolved fluorescence - (Abstract)

Tu, S., Reed, S.A., He, Y., Gehring, A.G. 2008. Detection of Salmonella Enteriditis from egg components using different immunomagnetic beads and time¿resolved fluorescence. [Abstract]p.2-42.

An antibody microarray, in multiwell plate format, for multiplex screening of foodborne pathogenic bacteria and biomolecules - (Peer Reviewed Journal)

Gehring, A.G., Albin, D.M., Reed, S.A., Tu, S., Brewster, J.D. 2008. An antibody microarray, in multiwell plate format, for multiplex screening of foodborne pathogenic bacteria and biomolecules. Analytical and Bioanalytical Chemistry. 391:497-506.

DETECTION OF SALMONELLA ENTERITIDIS IN LIQUID EGGS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE - (Abstract)

Tu, S., Gehring, A.G., Paoli, G. 2008. Detection of salmonella enteritidis in liquid eggs by immunomagnetic capture and time-resolved fluorescence. Meeting Abstract.Available: http://spie.org/x648.xml?product_id=786848&show/Abstracts=true&origin_id=x648&event_id=795339&start_at=41

A coupled anti-L. monocytgenes scFv immunomagnetic bead and PCR method for capture and detection of L. monocytogenes from food - (Abstract)

Paoli, G., Tu, S., Fan, Y., Shi, X. 2008. A coupled anti-L. monocytgenes scFv immunomagnetic bead and PCR method for capture and detection of L. monocytogenes from food . Meeting Abstract.

Application of horse-radish peroxidase linked chemiluminescence to determine the production mechanism of Shiga-like toxins by E. coli O157:H7 - (Proceedings/Symposium)

Tu, S., Uknalis, J., Gehring, A.G., He, Y. 2007. Application of horse-radish peroxidase linked chemiluminescence to determine the production mechanism of Shiga-like toxins by E. coli O157:H7. Proceedings of SPIE.#6761

Application of horse-radish peroxidase linked chemiluminescence to determine the production mechanism of Shiga-like toxins by E. coli O157:H7 - (Proceedings/Symposium)

Tu, S., Uknalis, J., Gehring, A.G., He, Y. 2007. Application of horse-radish peroxidase linked chemiluminescence to determine the production mechanism of Shiga-like toxins by E. coli O157:H7. Proceedings of SPIE.6761:67610C-1-67610C-9.

Multiple Pathogen Detection Using Biosensors: Advancements and Challenges - (Abstract)

Tu, S., Gehring, A.G., Paoli, G. 2007. Multiple Pathogen Detection Using Biosensors: Advancements and Challenges. [Abstract]p.59

SPR Biosensor for the Detection of L. monocytogenes using Phage Displayed Antibody - (Peer Reviewed Journal) - (05-Apr-07)

Detection of salmonella in liquid eggs by immunomagnetic capture and time-resolved fluorescence - (Peer Reviewed Journal)

Tu, S., Gehring, A.G., Paoli, G. 2007. Detection of salmonella in liquid eggs by immunomagnetic capture and time-resolved fluorescence. Journal of Rapid Methods and Automation in Microbiology. 15:107-119

IMMUNOMAGNETIC-ELECTROCHEMILUMINESCENT DETECTION OF ESCHERICHIA COLI 0157 IN GROUND BEEF AFTER PRE-ENRICHMENT IN BRILLIANT GREEN BILE BROTH, MODIFIED ESCHERICHIA COLI BROTH WITH OR WITHOUT NOVOBIOCIN, OR GRAM NEGATIVE BROTH - (Peer Reviewed Journal)

Feder, I.E., Wijey, C., Paoli, G., Crawford, C.G., Tu, S. 2007. Immunomagnetic-electrochemiluminescent detection of escherichia coli 0157 in ground beef after pre-enrichment in brilliant green bile broth, modified escherichia coli broth with or without novobiocin, or gram negative broth. Journal of Rapid Methods and Automation in Microbiology. 15:92-106.

ENZYME-LINKED IMMUNOMAGNETIC CHEMILUMINESCENCE INCORPORATING ANTI-H7 AND ANTI-0157 ANTIBODIES FOR THE DETECTION OF ESCHERICHIA COLI 0157:H7 - (Peer Reviewed Journal)

Gehring, A.G., Irwin, P.L., Reed, S.A., Tu, S. 2006. Enzyme-linked immunomagnetic chemiluminescence incorporating anti-h7 and anti-0157 antibodies for the detection of escherichia coli 0157:h7. Journal of Rapid Methods and Automation in Microbiology. 14:349-361.

FIBEROPTIC BIOSENSOR EMPLOYING ALEXA-FLUOR CONJUGATED ANTIBODY FOR DETECTION OF ESCHERICHIA COLI O157:H7 AND SHIGA-LIKE TOXINS - (Proceedings/Symposium)

Tu, S., Geng, T., Uknalis, J., Bhunia, A.K. 2006. Fiberoptic biosensor employing alexa-fluor conjugated antibody for detection of escherichia coli o157:h7 and shiga-like toxins. Proceedings of SPIE. 6:(6381:638106-1).

DETECTION AND POTENTIAL IDENTIFICATION OF ESCHERICHIA COLI 0157:H7 USING A MICROPARTICLE-BASED CHEMILUMINESCENT IMMUNOASSAY - (Abstract)

Gehring, A.G., Albin, D.M., Irwin, P.L., Tu, S. 2006. Detection and potential identification of escherichia coli 0157:H7 using a microparticle-based chemiluminescent immunoassay. Biosensors p. 101.

PIEZOELECTRIC EXCITED MILLIMETER-SIZED CANTILEVER (PEMC) SENSOR DETECTS ESCHERICHIA COLI 0157:H7 IN TWO-HOUR INCUBATED SAMPLES AT 4 CFU PER GRAM OF BEEF - (Peer Reviewed Journal)

Campbell, G.A., Uknalis, J., Tu, S., Mutharasan, R. 2006. Piezoelectric excited millimeter-sized cantilever (pemc) sensor detects escherichia coli 0157:h7 in two-hour incubated samples at 4 cfu per gram of beef. Biosensors and Bioelectronics. 22(2007)1296-1302

COMPARISON OF ENZYME-LINKED IMMUNOMAGNETIC CHEMILUMINESCENT WITH USF DAS BAM METHOD FOR THE DETECTION OF ESCHERICHIA COLI O157:H7 - (Peer Reviewed Journal)

Gehring, A.G., Albin, D.M., Irwin, P.L., Reed, S.A., Tu, S. 2006. Comparison of enzyme-linked immunomagnetic chemiluminescent with usf das bam method for the detection of escherichia coli o157:h7. Journal of Microbiological Methods. 67:527-533.

FIBER-OPTIC BIOSENSOR EMPLOYING ALEXA-FLUOR CONJUGATED ANTIBODY FOR DETECTION OF ESCHERICHIA COLI 0157:H7 FROM GROUND BEEF IN FOUR HOURS - (Peer Reviewed Journal)

Geng, T., Uknalis, J., Tu, S., Bhunia, A. 2006. Fiber-optic biosensor employing alexa-fluor conjugated antibody for detection of escherichia coli 0157:h7 from ground beef in four hours. Sensors. 6:796-807

SENSITIVE DETECTION OF ESCHERICHIA COLI 0157:H7 BY THE USE OF IMMUNOMAGNETIC AND FLUORESCENT BEADS - (Abstract)

Tu, S., Cooke, P.H., Paoli, G., Gehring, A.G. 2006. Sensitive detection of escherichia coli 0157:h7 by the use of immunomagnetic and fluorescent beads. Meeting Abstract.

DETECTION OF ESCHERICHIA COLI 0157:H7 THROUGH THE FORMATION OF SANDWICHED COMPLEXES WITH IMMUNOMAGNETIC AND FLUORESCENT BEADS - (Peer Reviewed Journal)

Tu, S., Golden, M., Cooke, P.H., Luce, L., Paoli, G., Gehring, A.G.2005 Detection of escherichia coli 0157:h7 through the formation of sandwiched complexes with immunomagnetic and fluorescent beads. Journal of Rapid Methods and Automation in Microbiology. 13:269-282

DETECTION OF ESCHERICHIA COLI 0157:H7 USING IMMUNO BEADS - (Proceedings/Symposium)

Tu, S., Gehring, A.G. 2005. Detection of escherichia coli 0157:h7 using immuno beads. SPIE Proceedings. 5996:1-7.

BIOSENSOR-BASED PROCESSES FOR DETECTION OF BACTERIA IN THE FOOD PROCESSING ENVIRONMENT - (Abstract)

Tu, S., Gehring, A.G., Paoli, G. 2005. Biosensor-based processes for detection of bacteria in the food processing environment. International Food Technology Meeting Abstracts. Paper No. 6-4.

LUMINESCENT METHODS TO DETECT VIABLE AND TOTAL TARGETED ESCHERICHIA COLI 0157:H7 SPIKED IN GROUND BEEF - (Peer Reviewed Journal)

Tu, S., Uknalis, J., Yamashoji, S., Gehring, A.G., Irwin, P.L. 2005. Luminescent methods to detect viable and total targeted escherichia coli 0157:h7 spiked in ground beef. Journal of Rapid Methods and Automation in Microbiology. 2005. 13. pp. 57-70.

BIOENERGETIC CONFIRMATION OF VIABLE PATHOGENS IN FOODS BY ATP-BIOLUMINESCENCE - (Peer Reviewed Journal)

Tu, S., Gehring, A.G., Irwin, P.L. 2005. Bioenergetic confirmation of viable pathogens in foods by atp-bioluminescence. Luminescence. p. 445-448.

OPTICAL METHODS FOR DETECTING ESCHERICHIA COLI 0157:H7 SPIKED ON CANTALOUPES - (Proceedings/Symposium)

Tu, S., Uknalis, J., Gehring, A.G. 2004. Optical methods for detecting escherichia coli 0157:h7 spiked on cantaloupes. Proceedings of SPIE. 5587. pp. 183-189.

TIME-RESOLVED FLUORESCENCE DETECTION OF TOXINS AND PATHOGENIC BACTERIA IN FOODS - (Peer Reviewed Journal)

Tu, S., Golden, M., Paoli, G., Gore, M., Gehring, A.G. 2004. Time-resolved fluorescence detection of toxins and pathogenic bacteria in foods. Journal of Rapid Methods and Automation in Microbiology. 12. pp. 247-258.

DETECTION OF VIABLE BACTERIA CELLS BY BIOLUMINESCENCE: A BIOENERGETIC APPROACH - (Peer Reviewed Journal)

Tu, S., Wijey, C., Paoli, G., Gehring, A.G., Irwin, P.L. 2004. Detection of viable bacteria cells by bioluminescence: a bioenergetic approach. Journal of Rapid Methods and Automation in Microbiology. V.12, #3, p. 207-219.

ENZYME-LINKED IMMUNOMAGNETIC ELECTROCHEMICAL DETECTION OF LIVE ESCHERICHIA COLI 0157:H7 IN APPLE JUICE - (Peer Reviewed Journal)

Gehring, A.G., Tu, S. 2005. Enzyme-linked immunomagnetic electrochemical detection of live escherichia coli 0157:h7 in apple juice. Journal of Food Protection. 2005. p.146-149.

ENZYME-LINKED IMMUNOMAGNETIC CHEMILUMINESCENT DETECTION OF ESCHERICHIA COLI 0157:H7 - (Peer Reviewed Journal)

Gehring, A.G., Irwin, P.L., Reed, S.A., Tu, S., Andreotti, P.E., Akhavan-Tafti, H., Handley, R.S. 2004. Enzyme-linked immunomagnetic chemiluminescent detection of escherichia coli 0157:h7. Journal of Immunological Methods 293. 2004. p. 97-106.

RAPID AND SENSITIVE MEASURES OF INTRACEULLUAR PH IN MAIZE ROOT TISSUE BY A-METHYL FLUORINATED ALANINES AND (19)F NMR SPECTROSCOPY - (Peer Reviewed Journal)

Pfeffer, P.E., Shachar-Hill, Y., Tu, S., Brauer, D. 2004. Rapid and sensitive measures of intraceulluar ph in maize root tissue by a-methyl fluorinated alanines and (19)f nmr spectroscopy. Physiologia Plantarum. v. 163. pp. 617-627.

BIOENERGETIC CONFIRMATION OF VIABLE PATHOGENS IN FOODS BY ATP-BIOLUMINESCENCE - (Abstract)

Tu, S. 2004. Bioenergetic confirmation of viable pathogens in foods by atp-bioluminescence. Meeting Abstract. Luminescence 19:182, 2004.

DYNAMIC FACTORS CONTROLLING THE PATHOGEN CAPTURE BY IMMUNO MAGNETIC BEADS - (Abstract)

Tu, S., Uknalis, J., Gore, M., Irwin, P.L. 2004. Dynamic factors controlling the pathogen capture by immuno magnetic beads. American Chemical Society National Meeting.

OPTICAL METHODS FOR DETECTING E. COLI 0157:H7 SPIKED ON CANTALOUPES - (Abstract)

Tu, S., Uknalis, J., Gehring, A.G. 2004. Optical methods for detecting e. coli 0157:h7 spiked on cantaloupes. International Society for Optical Engineering. Paper No. 6-183.

TIME-RESOLVED FLUORESCENCE DETECTION OF SHIGA-LIKE TOXINS PRODUCED BY ESCHERICHIA COLI 0157 AND NON-0157 IN GROUND BEEF - (Abstract)

Tu, S., Golden, M., Paoli, G., Gehring, A.G., Gore, M. 2004. Time-resolved fluorescence detection of shiga-like toxins produced by escherichia coli 0157 and non-0157 in ground beef. Meeting Abstract.

EFFECT OF BACTERIAL PROTEIN EXTRACTION REAGENT AND SODIUM AZIDE ON THE DETECTION OF ESCHERICHIA COLI 0157:H7 USING IMMUNOMAGNETIC-ELECTROCHEMILUMINESCENCE - (Peer Reviewed Journal)

Wijey, C., Crawford, C.G., Tu, S., Paoli, G. Effect of bacterial protein extraction reagent and sodium azide on the detection of escherichia coli 0157:h7 using immunomagnetic-electrochemiluminescence. Journal of Rapid Methods and Automation in Microbiology. 2004. 12. pp. 115-126.

BLOCKING NON-SPECIFIC ADSORPTION OF NATIVE FOOD-BORNE MICROORGANISMS BY IMMUNO-MAGNETIC BEADS WITH IOTA-CARRAGEENAN - (Peer Reviewed Journal)

IRWIN, P.L., GEHRING, A.G., TU, S., CHEN, C. BLOCKING NON-SPECIFIC ADSORPTION OF NATIVE FOOD-BORNE MICROORGANISMS BY IMMUNO-MAGNETIC BEADS WITH IOTA-CARRAGEENAN. CARBOHYDRATE RESEARCH. 2004. V. 339(3) pg. 613-621

RAPID DETECTION OF OUTBREAK ESCHERICHIA COLI 0157 AND SALMONELLA IN ALFALFA SPROUTS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLOURESCENCE - (Abstract)

TU, S., FETT, W.F., GEHRING, A.G., IRWIN, P.L. RAPID DETECTION OF OUTBREAK ESCHERICHIA COLI 0157 AND SALMONELLA IN ALFALFA SPROUTS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLOURESCENCE. MEETING ABSTRACT. 2003.

APPLICATIONS OF IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE TO DETECT OUTBREAK ESCHERICHIA COLI 0157 AND SALMONELLA IN ALFALFA SPROUTS - (Proceedings/Symposium)

TU, S., FETT, W.F., GEHRING, A.G., IRWIN, P.L. APPLICATIONS OF IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE TO DETECT OUTBREAK ESCHERICHIA COLI 0157 AND SALMONELLA IN ALFALFA SPROUTS. Proceedings of SPIE. 2003. V. 5271.

BIOSENSORS FOR THE DETECTION OF FOOD-BORNE PATHOGENS - (Abstract)

Paoli, G., Tu, S. 2003. Biosensors for the detection of food-borne pathogens. Eastern Food Science Conference.

KINETIC FACTORS AFFECTING THE BACTERIAL CAPTURE EFFICIENCY OF IMMUNO BEADS: A COMPARISON BETWEEN BEADS WITH DIFFERENT SIZE AND DENSITY - (Abstract)

TU, S., IRWIN, P.L., GEHRING, A.G. KINETIC FACTORS AFFECTING THE BACTERIAL CAPTURE EFFICIENCY OF IMMUNO BEADS: A COMPARISON BETWEEN BEADS WITH DIFFERENT SIZE AND DENSITY. MEETING ABSTRACT. 2003.

FACTORS AFFECTING THE BACTERIAL CAPTURE EFFICIENCY OF IMMUNO BEADS: A COMPARISON BETWEEN BEADS WITH DIFFERENT SIZE AND DENSITY - (Peer Reviewed Journal)

TU, S., UKNALIS, J., GORE, M., IRWIN, P.L., FEDER, I.E. FACTORS AFFECTING THE BACTERIAL CAPTURE EFFICIENCY OF IMMUNO BEADS: A COMPARISON BETWEEN BEADS WITH DIFFERENT SIZE AND DENSITY. JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY 11: 35-46. 2003.

SIMULTANEOUS DETECTION OF ESCHERICHIA COLI 0157:H7 AND SALMONELLA TYPHIMURIUM IN FOODS USING IMMUNOMAGNETIC CAPTURE AND LANTHANIDE TIME-RESOLVED FLUORESCENCE - (Abstract)

TU, S., GOLDEN, M., FEDER, I.E., IRWIN, P.L. SIMULTANEOUS DETECTION OF ESCHERICHIA COLI 0157:H7 AND SALMONELLA TYPHIMURIUM IN FOODS USING IMMUNOMAGNETIC CAPTURE AND LANTHANIDE TIME-RESOLVED FLUORESCENCE. MEETING ABSTRACT. 2003.

RAPID DETECTION OF OUTBREAK ESCHERICHIA COLI 0157 ADN SALMONELLA IN ALFALFA SPROUTS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE - (Peer Reviewed Journal)

TU, S., GOLDEN, M., FETT, W.F., GEHRING, A.G., IRWIN, P.L. RAPID DETECTION OF OUTBREAK ESCHERICHIA COLI 0157 ADN SALMONELLA IN ALFALFA SPROUTS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE. JOURNAL OF FOOD SAFETY.

ENZYME-LINKED IMMUNOMAGNETIC CHEMILUMINESCENT DETECTION OF ESCHERICHIA COLI 0157:H7 - (Abstract)

GEHRING, A.G., IRWIN, P.L., REED, S.A., TU, S. ENZYME-LINKED IMMUNOMAGNETIC CHEMILUMINESCENT DETECTION OF ESCHERICHIA COLI 0157:H7. PITTSBURGH CONFERENCE. 2003.

SIMULTANEOUS DETECTION OF ESCHERICHIA COLI 0157:H7 AND SALMONELLA TYPHIMURIUM IN FOODS USING IMMUNOMAGNETIC CAPTURE AND LANTHANIDE TIME-RESOLVED FLUORESCENCE - (Abstract)

TU, S., GOLDEN, M., IRWIN, P.L., GEHRING, A.G., FEDER, I.E. SIMULTANEOUS DETECTION OF ESCHERICHIA COLI 0157:H7 AND SALMONELLA TYPHIMURIUM IN FOODS USING IMMUNOMAGNETIC CAPTURE AND LANTHANIDE TIME-RESOLVED FLUORESCENCE. PITTSBURGH CONFERENCE. 2003.