Projects

IDENTIFYING AND MANIPULATING DETERMINANTS OF PHOTOSYNTHATE PRODUCTION AND PARTITIONING Appropriated (D)   Accession Number: 410467

IMPACT OF METHIONINE OXIDATION ON PROTEIN PHOSPHORYLATION Reimbursable (R)   Accession Number: 412483

IDENTIFICATION OF DETERMINANTS OF SEED PROTEIN CONTENT AND COMPOSITION Reimbursable (R)   Accession Number: 409608

OXIDATIVE STRESS AT ELEVATED CO2 AND IMPACT ON PROTEIN PHOSPHORYLATION Reimbursable (R)   Accession Number: 411064

GENETIC DETERMINANTS OF SEED PROTEIN AND OIL: CONTENT AND COMPOSITION Reimbursable (R)   Accession Number: 412441

SUCROSE SYNTHASE: ELUCIDATION OF COMPLEX POST-TRANSLATIONAL REGULATORY MECHANISMS Reimbursable (R)   Accession Number: 407981

REGULATION OF ASSIMILATE METABOLISM Specific Cooperative Agreement (S)   Accession Number: 408051

Publications

Increased protein carbonylation in leaves of Arabidopsis and soybean in response to elevated [CO2] - (Peer Reviewed Journal)

Qiu, Q., Huber, J., Booker, F.L., Jain, V.L., Leakey, A., Fiscus, E.L., Yau, P., Ort, D.R., Huber, S.C. 2008. Increased protein carbonylation in leaves of Arabidopsis and soybean in response to elevated [CO2]. Photosynthesis Research. Vol 97, pp. 155-166.

Cytokinin inhibits the proteasome-mediated degradation of carbonylated proteins in Arabidopsis leaves - (Peer Reviewed Journal)

Jain, V., Kaiser, W., Huber, S.C. 2008. Cytokinin inhibits the proteasome-mediated degradation of carbonylated proteins in Arabidopsis leaves. Plant Cell Physiology. 49:843-852.

Sucrose synthase oligomerization and F-actin association are regulated by sucrose concentration and phosphorylation - (Peer Reviewed Journal)

Duncan, K.A., Huber, S.C. 2007. Sucrose synthase oligomerization and F-actin association are regulated by sucrose concentration and phosphorylation. Plant Cell Physiology. 48:1612-1623.

METHIONINE OXIDATION AND PROTEIN PHOSPHORYLATION: INTERACTIVE PARTNERS IN SIGNALING? - (Proceedings/Symposium) - (18-Jul-07)

Functional Analysis and Phosphorylation Site Mapping of Leucine-Rich Repeat Receptor-Like Kinases - (Book/Chapter) - (01-Jun-07)

Sucrose synthase interaction with voltage-dependent anion channel suggests a potential role for the enzyme in inter-organellar signaling - (Abstract)

Chalivendra, S., Huber, S.C., Sachs, M.M., Rhoads, D. 2007. Sucrose synthase interaction with voltage-dependent anion channel suggests a potential role for the enzyme in inter-organellar signaling [abstract]. American Society of Plant Biologists Annual Meeting. 824.7

Metabolic signals control the selective degradation of sucrose synthase in maize leaves during de-etiolation - (Peer Reviewed Journal)

Qiu, Q., Hardin, S., Mace, J., Brutnell, T.P., Huber, S.C. 2007. Metabolic signals control the selective degradation of sucrose synthase in maize leaves during de-etiolation. Plant Physiology. 144(1):468-478.

Sucrose Synthase: Expanding Protein Function - (Peer Reviewed Journal)

Chalivendra, S.C., Huber, S.C., Sachs, M.M., Rhoads, D.M. 2007. Sucrose Synthase: Expanding Protein Function. Plant Signaling and Behavior. 2(1):28-29.

The Three Maize Sucrose synthase Isoforms Differ in Distribution, Localization, and Phosphorylation - (Peer Reviewed Journal)

Duncan, K.A., Hardin, S.C., Huber, S.C. 2006. The Three Maize Sucrose synthase Isoforms Differ in Distribution, Localization, and Phosphorylation. Plant Cell Physiology. 47:959-971.

The Three Maize Sucrose synthase Isoforms Differ in Distribution, Localization, and Phosphorylation - (Peer Reviewed Journal)

Duncan, K.A., Hardin, S.C., Huber, S.C. 2007. The Three Maize Sucrose synthase Isoforms Differ in Distribution, Localization, and Phosphorylation. Plant Cell Physiology. 47:959-971.

Determination of structural requirements and probable regulatory effectors for membrane association of maize sucrose synthase 1 - (Peer Reviewed Journal)

Hardin, S.C., Duncan, K.A., Huber, S.C. 2006. Determination of structural requirements and probable regulatory effectors for membrane association of maize sucrose synthase 1. Plant Physiology. 141:1106-1119.

MOLECULAR MECHANISMS OF RECEPTOR KINASE ACTION IN BRASSINOSTEROID SIGNAL TRANSDUCTION - (Proceedings/Symposium)

Wang, X., Goshe, M.B., Kota, U., Hardin, S., Huber, S.C., Li, J., Clouse, S.D. 2006. Molecular mechanisms of receptor kinase action in brassinosteroid signal transduction. Iowa State University Biochemistry Biophysics and Molecular Biology Symposium, July 10-14, 2006, Ames, Iowa. p. 32.

MITOCHONDRIAL LOCALIZATION AND A PUTATIVE SIGNALING FUNCTION OF SUCROSE SYNTHASE IN MAIZE - (Peer Reviewed Journal)

Chalivendra, S.C., Palaniappan, A., Duncan, K., Rhoads, D.M., Huber, S.C., Sachs, M.M. 2006. Mitochondrial localization and a putative signaling function of sucrose synthase in maize. Journal of Biological Chemistry. 281:15625-15635.

Polycations Globally Enhance Binding of 14-3-3 omega to Target Proteins in Spinach Leaves - (Peer Reviewed Journal)

Shen, W., Huber, S.C. 2006. Polycations Globally Enhance Binding of 14-3-3 omega to Target Proteins in Spinach Leaves. Plant Cell Physiology. 47:764-771.

SUCROSE SYNTHASE INTERACTION WITH THE VOLTAGE-DEPENDENT ANION CHANNEL SUGGESTS A POTENTIAL ROLE FOR THE ENZYME IN INTER-ORGANELLAR SIGNALING - (Abstract)

Chalivendra, S.C., Huber, S.C., Sachs, M.M., Rhoads, D.M. 2006. Sucrose synthase interaction with the voltage-dependent anion channel suggests a potential role for the enzyme in inter-organellar signaling. American Society of Plant Biologists [abstract]. Paper no. 36027. Available: http://abstracts.aspb.org/pb2006/public/P36/P36027.html.

POSSIBLE LINK BETWEEN METHIONINE OXIDATION AND PROTEIN PHOSPHORYLATION - (Abstract)

Hardin, S., Tien, M., Huber, S.C. 2006. Possible link between methionine oxidation and protein phosphorylation [abstract]. American Society of Plant Biologists. Paper no. 07011. Available: http://abstracts.aspb.org/pb2006/public/P07/P07011.html.

SUCROSE SYNTHASE (SUS) OLIGOMERIZATION IS REGULATED BY SUCROSE LEVELS WITHIN PLANT CELLS - (Abstract)

Duncan, K.A., Hardin, S., Huber, S.C. 2006. Sucrose synthase (SUS) oligomerization is regulated by sucrose levels within plant cells [abstract]. American Society of Plant Biologists. Abstract no. P24012. Available: http://abstracts.aspb.org/pb2006/public/P24/P24012.html.

CORRELATION BETWEEN CYTOPLASMIC DOMAIN SEQUENCE AND AUTOPHOSPHORYLATION AMONG ARABIDOPSIS LEUCINE-RICH REPEAT RECEPTOR-LIKE KINASES - (Abstract)

Hardin, S., Dhundaydham, M., Clouse, S.D., Huber, S.C. 2006. Correlation between cytoplasmic domain sequence and autophosphorylation among arabidopsis leucine-rich repeat receptor-like kinases. American Society of Plant Biologists [abstract]. Paper no. P36018. Available: http://abstracts.aspb.org/pb2006/public/P36/P36018.html.

ISOFORM-SPECIFIC BINDING OF 14-3-3 PROTEINS TO NITRATE REDUCTASE AND THE BRASSINOSTEROID INSENSITIVE 1 RECEPTOR KINASE SIGNALING COMPLEX - (Abstract)

Oh, M., Huber, J.L., Wang, X., Ferl, R., Clouse, S.D., Huber, S.C. 2006. Isoform-specific binding of 14-3-3 proteins to nitrate reductase and the brassinosteroid insensitive 1 receptor kinase signaling complex [abstract]. American Society of Plant Biologists [abstract]. Paper no. 36033. Available: http://abstracts.aspb.org/pb2006/public/P36/P36033.html,

HOW WILL THE GENE EXPRESSION PROFILE, BIOCHEMISTRY AND PHYSIOLOGY OF SOYBEAN LEAVES RESPOND TO GROWTH AT ELEVATED [CO2] UNDER OPEN-AIR FIELD CONDITIONS? - (Abstract)

Leakey, A.D.B., Xu, F., Gillespie, K.M., Heady, L., Qiu, Q., Rupassara, S., Ainsworth, E.A., Bohnert, H.J., Huber, S.C., Long, S.P., Rogers, A., Ort, D.R. 2006. How will the gene expression profile, biochemistry and physiology of soybean leaves respond to growth at elevated [CO2] under open-air field conditions? American Society of Plant Biologists Abstracts. Available: http://abstracts.aspb.org/pb2006/public/P01/P01013.html.

Exploring the role of protein phosphorylation in plants: from signaling to metabolism - (Proceedings/Symposium)

Huber, S.C. 2006. Exploring the role of protein phosphorylation in plants: from signaling to metabolism. Biochemical Society Transactions. 35:28-32.

OLEATE DESATURASE ENZYMES OF SOYBEAN: EVIDENCE OF REGULATION THROUGH DIFFERENTIAL STABILITY AND PHOSPHORYLATION. - (Peer Reviewed Journal)

Tang, G., Novitzky, W.P., Griffin, C., Huber, S.C., Dewey, R. 2005. Oleate desaturase enzymes of soybean: evidence of regulation through differential stability and phosphorylation. Plant Journal. 44:433-446.

REGULATION OF STORAGE PRODUCT ACCUMULATION IN SOYBEAN SEED - (Proceedings/Symposium)

Marsolais, F., Hernández-Sebastià, C., Huber, S.C. 2005. Regulation of storage product accumulation in soybean seed. In: Comp. Biochem. Physiol. A141, S289 (P7.89). Abstracts of the Annual Main Meeting of the Society for Experimental Biology, 11-15th July 2005, Universitat Autonoma de Barcelona, Barcelona, Spain.

INTERACTION OF ARABIDOPSIS BRASSINOSTEROID-INSENSITIVE BETA RECEPTOR KINASE WITH A HOMOLOG OF MAMMALIAN TGF RECEPTOR INTERACTING PROTEIN. - (Peer Reviewed Journal)

Ehsan, H., Ray, W.K., Phinney, B., Wang, X., Huber, S.C., Clouse, S.D. 2005. Interaction of Arabidopsis BRASSINOSTEROID-INSENSITIVE 1 receptor kinase with a homolog of mammalian TGF receptor interacting protein. Plant Journal. 43:251-261.

IDENTIFICATION AND FUNCTIONAL ANALYSIS OF IN VIVO PHOSPHORYLATION SITES OF THE ARABIDOPSIS BRASSINOSTEROID INSENSITIVE 1 RECEPTOR KINASE - (Peer Reviewed Journal)

Wang, X., Goshe, M.B., Soderblom, E.J., Phinney, B.S., Kuchar, J., Li, J., Asami, T., Huber, S.C., Clouse, S.D. 2005. Identification and functional analysis of in vivo phosphorylation sites of the arabidopsis brassinosteroid insensitive 1 receptor kinase. The Plant Cell. 17:1685-1703.

MOLECULAR MECHANISMS OF EARLY EVENTS IN BRASSINOSTEROID SIGNAL TRANSDUCTION. - (Abstract)

Wang, X., Goshe, M.B., Soderblom, E.J., Phinney, B.S., Kuchar, J.A., Li, J., Asami, T., Yoshida, S., Huber, S.C., Clouse, S.D. 2005. Molecular mechanisms of early events in brassinosteroid signal transduction [abstract]. Plant Biology Annual Meeting. Available: http://abstracts/aspb.org/pb2005/public/P66/7861.html.

THE THIRD SUCROSE SYNTHASE IN <1>ZEA MAYS(SUS3) IS A UBIQUITOUS PROTEIN THAT IS PHOSPHORYLATED AND MEMBRANE ASSOCIATED. - (Abstract)

Duncan, K.A., Hardin, S.C., Huber, S.C. The third sucrose synthase in <1>zea mays(sus3) is a ubiquitous protein that is phosphorylated and membrane associated [abstract]. Plant Biology Annual Meeting. Available: http://abstracts.aspb.org/pb2005/public/P56/7486.html.

THE ASSOCIATION OF SUCROSE SYNTHASE WITH INTRACELLULAR MEMBRANES MAY INVOLVE A REGION WITH SIMILARITY TO A PLECKSTRIN HOMOLOGY DOMAIN. - (Abstract)

Hardin, S.C., Duncan, K.A., Huber, S.C. 2005. The association of sucrose synthase with intracellular membranes may involve a region with similarity to a pleckstrin homology domain [abstract]. Plant Biology Annual Meeting. Available: http://abstracts.aspb.org/pb2005/public/P56/7488.html.

NEW INTRACELLULAR LOCATIONS AND PUTATIVE NOVEL FUNCTIONS FOR MAIZE SUCROSE SYNTHASES. - (Abstract)

Chalivendra, S.C., Palaniappan, A., Duncan, K., Huber, S.C., Sachs, M.M. 2005. New intracellular locations and putative novel functions for maize sucrose synthases [abstract]. Plant Biology Annual Meeting. Available: http://abstracts.aspb.org/pb2005/public/P51/7674.html.

IDENTIFICATION OF THE MAJOR LEAF PROTEINS SUBJECT TO O-GLCNAC POSSTRANSLATIONAL MODIFICATION. - (Abstract)

Qiu, Q., Huber, J.L., Huber, S.C. 2005. Identification of the major leaf proteins subject to o-glcnac posstranslational modification [abstract]. Plant Biology Annual Meeting. Available: http://abstracts.aspb.org/pb2005/public/P69/7476.html.

PROTEIN OXIDATION IN LEAVES OF ARABIDOPSIS AND SOYBEAN: IMPLICATIONS FOR PLANT RESPONSE TO ELEVATED CO2. - (Abstract)

Qiu, Q., Huber, J.L., Portis Jr, A.R., Huber, S.C. 2005. Protein oxidation in leaves of arabidopsis and soybean: implications for plant response to elevated CO2. Plant Biology Annual Meeting. Available: http://abstracts/aspb.org/pb2005/public/P36/7475.html.

PROTEASOME ACTIVITY AND THE POST-TRANSLATIONAL CONTROL OF SUCROSE SYNTHASE STABILITY IN MAIZE LEAVES - (Abstract)

Hardin, S.C., Huber, S.C. 2004. Proteasome activity and the post-translational control of sucrose synthase stability in maize leaves [abstract]. Plant Physiology and Biochemistry. 42:197-208.

IDENTIFICATION OF A NEW MOTIF FOR CDPK PHOSPHORYLATION IN VITRO THAT SUGGESTS ACC SYNTHASE MAY BE A CDPK SUBSTRATE - (Peer Reviewed Journal)

Sebastia, C.H., Hardin, S.C., Clouse, S.C., Kieber, J.J., Huber, S.C. 2004. Identification of A New Motif for CDPK Phosphorylation In Vitro That Suggests ACC Synthase May Be A CDPK Substrate. Archives Of Biochemistry and Biophysics. 428:81-91.

METABOLIC PROFILING OF AMINO ACIDS IN SOYBEAN DEVELOPING SEEDS: POSSIBLE ROLE OF ASPARAGINE IN THE CONTROL OF STORAGE PRODUCT ACCUMULATION - (Peer Reviewed Journal)

Sebastia, C.H., Marsolais, F., Saravitz, C., Israel, D.W., Dewey, R.E., Huber, S.C. 2005. Metabolic profiling of amino acids in soybean developing seeds: possible role of asparagine in the control of storage product accumulation. Journal of Experimental Botany.56:1951-1964.

NUMEROUS POST-TRANSLATIONAL MODIFICATIONS PROVIDE OPPORTUNITES FOR THE INTRICATE REGULATION OF METABOLIC ENZYMES AT MULTIPLE LEVELS - (Peer Reviewed Journal)

Huber, S.C., Hardin, S.C. 2004. Numerous post-translational modifications provide opportunites for the intricate regulation of metabolic enzymes at multiple levels. Plant Biology. 7:318-322.

PHOSPHORYLATION OF THE AMINO-TERMINUS OF MAIZE SUCROSE SYNTHASE IN RELATION TO MEMBRANE ASSOCIATION AND ENZYME ACTIVITY - (Peer Reviewed Journal)

Hardin, S.C., Winter, H., Huber, S.C. 2004. Phosphorylation of the amino-terminus of maize sucrose synthase in relation to membrane association and enzyme activity. Plant Physiology. 134:1427-1438.

PROTEASOME ACTIVITY AND THE POST-TRANSLATIONAL CONTROL OF SUCROSE SYNTHASE STABILITY IN MAIZE LEAVES - (Peer Reviewed Journal)

HARDIN, S.C., HUBER, S.C. 2004. Proteasome activity and the post-translational control of sucrose synthase stability in maize leaves. Plant Physiology and Biochemistry. 42:197-208.

PROTEASOME ACTIVITY AND THE POST-TRANSLATIONAL CONTROL OF SUCROSE SYNTHASE STABILITY IN MAIZE LEAVES. - (Peer Reviewed Journal)

Hardin, S., Huber, S.C. 2004. Proteasome activity and the post-translational control of sucrose synthase stability in maize leaves. Plant Physiology and Biochemistry. 42:197-208.

PHOSPHORYLATION OF METABOLIC ENZYMES: EFFECTS ON ACTIVITY, LOCALIZATION AND DEGRADATION - (Abstract)

Huber, S.C. 2003. Phosphorylation of metabolic enzymes: effects on activity, localization and degradation [abstract]. Molecular and Cellular Proteomics. 2:679.

"PHOSPHORYLATION OF SUCROSE SYNTHASE AT SERINE 170: OCCURRENCE AND POSSIBLE ROLE AS A SIGNAL FOR PROTEOLYSIS" - (Peer Reviewed Journal)

Hardin, S.C., Tang, G.Q., Scholz, A., Holtgraewe, D., Winter, H., Huber, S.C. 2003. "Phosphorylation of Sucrose Synthase at Serine 170: Occurrence and Possible Role As a Signal for Proteolysis" [abstract]. Plant Journal. 35:588-603.

AN ARABIDOPSIS PROTEIN WITH SEQUENCE SIMILARITY TO ANIMAL TGF-BETA RECEPTOR INTERACTING PROTEIN IS PHOSPHORYLATED BY THE BR11 RECEPTOR KINASE IN VITRO - (Abstract)

Ehsan, H., Ray, W.K., Huber, S.C., Clouse, S.D. 2003. An arabidopsis protein with sequence similarity to animal tgf-beta receptor interacting protein is phosphorylated by the br11 receptor kinase in vitro [abstract]. American Society of Plant Biologists. Available: http://abstracts.aspb.org/pb2003/public/M12/5068.html

DO AMINO ACIDS REGULATE TRANSCRIPTION OF LIPOGENIC GENES IN DEVELOPING SOYBEAN SEEDS? - (Abstract)

Hernandez, C., Saravitz, C., Zhang, P., Israel, D., Dewey, R., Huber, S.C. 2003. Do amino acids regulate transcription of lipogenic genes in developing soybean seeds? [abstract]. International Congress of Plant Molecular Biology. Paper No. 3620.

THE C-TERMINAL TAIL OF ARABIDOPSIS 14-3 FUNCTIONS AS AN AUTOINHIBITOR AND MAY CONTAIN A TENTH A-HELIX - (Peer Reviewed Journal)

Shen, W., Clark, C., Huber, S.C. 2003. The c-terminal tail of arabidopsis 14-3 functions as an autoinhibitor and may contain a tenth a-helix. Plant Journal. 34:473-484.

A NOVEL C-TERMINAL PROTEOLYTIC PROCESSING OF CYTOSOLIC PYRUVATE KINASE, ITS PHOSPHORYLATION AND DEGRADATION BY THE PROTEASOME IN DEVELOPING SOYBEAN SEEDS - (Peer Reviewed Journal)

Tang, G., Hardin, S.C., Dewey, R., Huber, S.C. 2003. A novel c-terminal proteolytic processing of cytosolic pyruvate kinase, its phosphorylation and degradation by the proteasome in developing soybean seeds. Plant Journal. 34:77-95.

PROTEIN PHOSPHORYLATION AS A POSSIBLE TRIGGER FOR DEGRADATION OF METABOLIC ENZYMES - (Abstract)

HUBER, S.C., TANG, G.Q., HARDIN, S.C. 2003. Protein Phosphorylation As A Possible Trigger For Degradation of Metabolic Enzymes [abstract]. Current Topics In Plant Biochemistry, Physiology and Molecular Biology. Plant Protein Phosphorylation-Dephosphorylation. 21:1415.