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![](https://webarchive.library.unt.edu/eot2008/20090117214746im_/http://www.ars.usda.gov/incme/images/Research_head.gif) |
Research Project:
Characterization, Production, and Utilization of Phytochemicals from Agricultural Products
Location: New Crops and Processing Technology Research
Title: Beta-conglycinin embeds active peptides 1 that inhibit lipid accumulation in 3T3-L1 adipocytes in vitro
Authors
![item](https://webarchive.library.unt.edu/eot2008/20090117214746im_/http://www.ars.usda.gov/incme/images/bullet.gif) | Martinez-Villaluenga, Cristina - UNIVERSITY OF ILLINOIS-UR | ![item](https://webarchive.library.unt.edu/eot2008/20090117214746im_/http://www.ars.usda.gov/incme/images/bullet.gif) | Bringe, Neal - THE MONSANTO COMPANY | ![item](https://webarchive.library.unt.edu/eot2008/20090117214746im_/http://www.ars.usda.gov/incme/images/bullet.gif) |
Berhow, Mark
| ![item](https://webarchive.library.unt.edu/eot2008/20090117214746im_/http://www.ars.usda.gov/incme/images/bullet.gif) | Gonzalez DE Mejia, Elvira - UNIVERSITY OF ILLINOIS-UR |
Submitted to: Journal of Agricultural and Food Chemistry
Publication Type:
Peer Reviewed Journal
Publication Acceptance Date: September 18, 2008
Publication Date: November 26, 2008
Citation: Martinez-Villaluenga, C., Bringe, N.A., Berhow, M.A., Gonzalez De Mejia, E. 2008. Beta-conglycinin embeds active peptides 1 that inhibit lipid accumulation in 3T3-L1 adipocytes in vitro. Journal of Agricultural and Food Chemistry 56:10533-10543.
Interpretive Summary: Obesity is a worldwide health concern because it is a well recognized predictor of premature mortality. The objective was to identify soybean varieties that have improved potential to inhibit fat accumulation in adipocytes by testing the effects of soy hydrolysates having a range of protein subunit compositions on lipid accumulation and adiponectin expression in model fat cell cultures. The results showed that differences in the protein distribution of 15 soy genotypes led to different potential for reduction of fat accumulation. It was feasible to differentiate soy varieties based on the potential for reduction of fat of their proteins using a statistical model and a cell-based assay in vitro. Furthermore, beta-conglycinin embeds more peptides than glycinin subunits that inhibit lipid accumulation and induce adiponectin in the cell models. Therefore, soy ingredients containing beta-conglycinin may be important food components for control of lipid accumulation in adipose tissue. This research is leading to an understanding of what components in soy foods can be used to slow or halt the development of obesity in humans and animals.
Technical Abstract: Obesity is a worldwide health concern because it is a well recognized predictor of premature mortality. The objective was to identify soybean varieties that have improved potential to inhibit fat accumulation in adipocytes by testing the effects of soy hydrolysates having a range of protein subunit compositions on lipid accumulation and adiponectin expression in 3T3-L1 adipocytes. The results showed that differences in the protein distribution of 15 soy genotypes led to different potential for reduction of fat accumulation. The inhibition of lipid accumulation of soy alcalase hydrolysates in 3T3-L1 adipocytes ranged from 29% to 46%. Soy hydrolysates made from genotypes with 45.3 ± 3.3% of total protein as beta-conglycinin, on average, showed significantly higher inhibition of lipid accumulation compared to those with 24.7 ± 1.5% of extracted total protein a beta-conglycinin. Moreover, after in vitro simulated digestion with pepsin pancreatin of the soy alcalase hydrolysates, 86% of the original activity remained. Adiponectin expression was induced in 3T3-L1 adipocytes treated with 15 soy hydrolysates up to 2.49 and 2.63 fold for high and low molecular weight adiponectin, respectively. The inhibition of lipid accumulation calculated from a Partial Least Square (PLS) analysis model correlated well with experimental data (R2 = 0.91). In conclusion, it was feasible to differentiate soy varieties based on the potential for reduction of fat of their proteins using a statistical model and a cell-based assay in vitro. Furthermore, beta-conglycinin embeds more peptides than glycinin subunits that inhibit lipid accumulation and induce adiponectin in 3T3-L1 adipocytes. Therefore, soy ingredients containing beta-conglycinin may be important food components for control of lipid accumulation in adipose tissue.
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Last Modified: 01/16/2009
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