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Development of an Immunobiosensor for Detection of PCBs

EPA Contract Number: 68D60022
Title: Development of an Immunobiosensor for Detection of PCBs
Investigators: Ray, Bryan L.
Small Business: SymBiotech Inc.
EPA Contact: Manager, SBIR Program
Phase: I
Project Period: September 1, 1996 through March 1, 1997
Project Amount: $70,000
RFA: Small Business Innovation Research (SBIR) - Phase I (1995)
Research Category: Ecological Indicators/Assessment/Restoration , SBIR - Monitoring and Analysis

Description:

This proposal aims to develop an immuno-based biosensor for detecting PCBs in soil at 50ng/g and in water at 1 ng/L when combined with a sample concentration method to be developed in this proposal. The immunobiosensor will employ a photodetection system comprising bacteriorhodopsin, an integral membrane protein of the purple membrane of Halobacterium halobium. Bacteriorhodopsin pumps protons across the membrane when exposed to light. This response to light can be electronically monitored when purple membranes are deposited onto an electrode. Specific affinity interactions of antibodies with haptens (e.g., PCBs) can alter the photoresponse of these purple membranes. The ability of antibody/hapten inter-actions to quantitatively alter this photoresponse forms the basis for the immunobiosensor technology. The feasability of this technology will be determined.

Supplemental Keywords:

small business, SBIR, engineering, chemistry, immunoassay, hazardous waste , Ecosystem Protection/Environmental Exposure & Risk, Toxics, Water, Scientific Discipline, Waste, RFA, Hazardous Waste, Environmental Engineering, pesticides, Environmental Chemistry, Contaminated Sediments, Hazardous, Monitoring/Modeling, Environmental Monitoring, membrane technology, photodetection system, monitoring, biosensors, PCB detoxification, biosensing system, polychlorinated biphenyls (PCBs), contaminated soil, analytical chemistry, bioassay, soil, PCB, hazardous chemicals, biosensor, pesticide residue, immunobisensor, biosensing, soil sediment

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.

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