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Identifying the Signature of the Natural Attenuation in the Microbial Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and “Bug Traps”

EPA Grant Number: R830633C007
Subproject: this is subproject number 007 , established and managed by the Center Director under grant R830633
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: IPEC University of Tulsa (TU)
Center Director: Sublette, Kerry L.
Title: Identifying the Signature of the Natural Attenuation in the Microbial Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and “Bug Traps”
Investigators: Sublette, Kerry L. , Ford, Laura P. , Kolhatkar, R. , Peacock, A. , White, D.
Institution: University of Tulsa
EPA Project Officer: Krishnan, Bala S.
Project Period: May 1, 2003 through April 30, 2004
Project Amount: Refer to main center abstract for funding details.
RFA: Integrated Petroleum Environmental Consortium (IPEC) (1999)
Research Category: Targeted Research

Description:

Objective:

Specifically we proposed to search for the signature of MTBE intrinsic bioremediation in the microbial ecology of the contaminated groundwater. BP, in collaboration with EPA, recently conducted an extensive survey of 74 BP retail sites with gasoline spills. Some of these sites indicated MTBE attenuation and others did not. Working with BP we will investigate and compare the in situ microbial ecologies of a subset of these sites using "bug traps" which concentrate organisms for analysis and provide a time-integrated picture of the subsurface microbial community. Further we will supplement this field work with an analysis of the microbial ecology of microcosms currently operated in the laboratory of Dr. Joe Suflita (University of Oklahoma). Microcosms positive for MTBE biodegradation will be sampled using the same bug traps used in the field sampling.

It is hypothesized that the in situ microbiota contain a signature of past and present MTBE exposure and utilization. A comprehensive measure of the in situ microbial ecology of a site will yield the identity of this signature. We maintain that a definition of MTBE natural attenuation in terms of a site's microbial ecology is direct and definitive, whereas a definition in terms of contaminant chemistry and/or geochemistry or hydrology is indirect and inferential. It is well established that indigenous microbes generate various compounds within their cellular structure (biomarkers) that reflect in situ conditions. Therefore, the microbial community contains a record of the sum microbial response to the environment that is written from changes in the biochemistry of individual organisms. We propose that these responses, in association with knowledge of available electron acceptors and donors of a site, will define the signature of a successful MTBE natural attenuation process.


Progress and Final Reports:
Final Report


Main Center Abstract and Reports:
R830633    IPEC University of Tulsa (TU)

Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R827015C001 Evaluation of Road Base Material Derived from Tank Bottom Sludges
R827015C002 Passive Sampling Devices (PSDs) for Bioavailability Screening of Soils Containing Petrochemicals
R827015C003 Demonstration of a Subsurface Drainage System for the Remediation of Brine-Impacted Soil
R827015C004 Anaerobic Intrinsic Bioremediation of Whole Gasoline
R827015C005 Microflora Involved in Phytoremediation of Polyaromatic Hydrocarbons
R827015C006 Microbial Treatment of Naturally Occurring Radioactive Material (NORM)
R827015C007 Using Plants to Remediate Petroleum-Contaminated Soil
R827015C008 The Use of Nitrate for the Control of Sulfide Formation in Oklahoma Oil Fields
R827015C009 Surfactant-Enhanced Treatment of Oil-Contaminated Soils and Oil-Based Drill Cuttings
R827015C010 Novel Materials for Facile Separation of Petroleum Products from Aqueous Mixtures Via Magnetic Filtration
R827015C011 Development of Relevant Ecological Screening Criteria (RESC) for Petroleum Hydrocarbon-Contaminated Exploration and Production Sites
R827015C012 Humate-Induced Remediation of Petroleum Contaminated Surface Soils
R827015C013 New Process for Plugging Abandoned Wells
R827015C014 Enhancement of Microbial Sulfate Reduction for the Remediation of Hydrocarbon Contaminated Aquifers - A Laboratory and Field Scale Demonstration
R827015C015 Locating Oil-Water Interfaces in Process Vessels
R827015C016 Remediation of Brine Spills with Hay
R827015C017 Continuation of an Investigation into the Anaerobic Intrinsic Bioremediation of Whole Gasoline
R827015C018 Using Plants to Remediate Petroleum-Contaminated Soil
R827015C019 Biodegradation of Petroleum Hydrocarbons in Salt-Impacted Soil by Native Halophiles or Halotolerants and Strategies for Enhanced Degradation
R827015C020 Anaerobic Intrinsic Bioremediation of MTBE
R827015C021 Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
R827015C022 A Continuation: Humate-Induced Remediation of Petroleum Contaminated Surface Soils
R827015C023 Data for Design of Vapor Recovery Units for Crude Oil Stock Tank Emissions
R827015C024 Development of an Environmentally Friendly and Economical Process for Plugging Abandoned Wells
R827015C025 A Continuation of Remediation of Brine Spills with Hay
R827015C026 Identifying the Signature of the Natural Attenuation of MTBE in Goundwater Using Molecular Methods and "Bug Traps"
R827015C027 Identifying the Signature of Natural Attenuation in the Microbial Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and "Bug Traps"
R827015C028 Using Plants to Remediate Petroleum-Contaminated Soil: Project Continuation
R827015C030 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
R827015C031 Evaluation of Sub-micellar Synthetic Surfactants versus Biosurfactants for Enhanced LNAPL Recovery
R827015C032 Utilization of the Carbon and Hydrogen Isotopic Composition of Individual Compounds in Refined Hydrocarbon Products To Monitor Their Fate in the Environment
R830633 Integrated Petroleum Environmental Consortium (IPEC)
R830633C001 Development of an Environmentally Friendly and Economical Process for Plugging Abandoned Wells (Phase II)
R830633C002 A Continuation of Remediation of Brine Spills with Hay
R830633C003 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
R830633C004 Evaluation of Sub-micellar Synthetic Surfactants versus Biosurfactants for Enhanced LNAPL Recovery
R830633C005 Utilization of the Carbon and Hydrogen Isotopic Composition of Individual Compounds in Refined Hydrocarbon Products To Monitor Their Fate in the Environment
R830633C006 Evaluation of Commercial, Microbial-Based Products to Treat Paraffin Deposition in Tank Bottoms and Oil Production Equipment
R830633C007 Identifying the Signature of the Natural Attenuation in the Microbial Ecology of Hydrocarbon Contaminated Groundwater Using Molecular Methods and “Bug Traps”
R830633C008 Using Plants to Remediate Petroleum-Contaminated Soil: Project Continuation
R830633C009 Use of Earthworms to Accelerate the Restoration of Oil and Brine Impacted Sites
X832428C001 Effective Stormwater and Sediment Control During Pipeline Construction Using a New Filter Fence Concept
X832428C002 Paraffin Control in Oil Wells Using Anaerobic Microorganisms
X832428C003 Fiber Rolls as a Tool for Re-Vegetation of Oil-Brine Contaminated Watersheds

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.

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