Technical Abstract: Tissues from Coffea arabica, C. liberica, C. dewevrei and C. congensis collected in Hawaii, Colombia and at a local plant nursery in Maryland were sampled for the presence of fungal endophytes. Surface sterilized tissues including roots, leaves, stems, and various part of the berry were plated on yeast malt agar. DNA was extracted from a set of isolates visually recognized as Penicillium and the internal transcribed spacer region and partial LSU-rDNA was amplified and sequenced. Comparison of DNA sequences with GenBank and unpublished sequences revealed the presence of ten known Penicillium species: P. brevicompactum, P. brocae , P. citrinum, P. coffeae, P. crustosum, P. janthinellum, P. olsonii, P. oxalicum, P. sclerotiorum and P. steckii, as well as three possibly undescribed species near P. dendriticum, P. diversum, and P. roseopurpureum. Ochratoxin A production was positive in only four isolates, one isolate each of P. brevicompactum, P. crustosum, P. olsonii, and P. oxalicum. The role that these endophytes play in the biology of the coffee plant remains enigmatic.