Projects

PATHWAY ENGINEERING OF FUNGI FOR IMPROVED BIOPROCESS APPLICATIONS Appropriated (D)   Accession Number: 408515

Publications

Inhibition of Rhizopus lactate dehydrogenase by fructose 1,6-bisphosphate - (Peer Reviewed Journal) - (17-Oct-08)

A novel family of carbohydrate esterases based on the gene identification for the Hypocrea jecorina acetyl esterase - (Peer Reviewed Journal) - (07-Oct-08)

Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain - (Abstract)

Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.

Bacterial Endosymbiosis is widely present among Zygomycetes but does not contribute to Mucormycosis Pathogenesis - (Peer Reviewed Journal) - (06-Apr-08)

High Affinity Iron Permease is Required for Virulence of Rhizopus oryzae - (Abstract)

Lin, L., Spellberg, B.J., Fu, Y., Skory, C.D., Gebremariam, T., Husseiny, M.I., Edwards, Jr, J.E., Ibrahim, A.S. 2007. High Affinity Iron Permease is Required for Virulence of Rhizopus oryzae [abstract]. The American Society for Microbiology's 47th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC). Presentation #B-1444.

Bacterial Endosymbiosis Does Not Contribute to Mucormycosis Pathogenesis - (Abstract)

Ibrahim, A.S., Gebremariam, T., Liu, M., Chamilos, G., Kontoyiannis, D.P., Kwon-Chung, K.J., Fu, Y., Skory, C.D., Edwards, Jr., J.E., Spellberg, B.J. 2007. Bacterial Endosymbiosis Does Not Contribute to Mucormycosis Pathogenesis [abstract]. The American Society for Microbiology's 47th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC). Presentation #B-1443.

The iron chelator deferasirox protects mice from mucormycosis through iron starvation - (Peer Reviewed Journal)

Ibrahim, A.S., Gebermariam, T., Fu, Y., Lin, L., Husseiny, M.I., French, S.W., Schwartz, J., Skory, C.D., Edwards, J.E., Spellberg, B.J. 2007. The iron chelator deferasirox protects mice from mucormycosis through iron starvation. Journal of Clinical Investigation. 117(9):2649-2657.

Native and Modified Lactate Dehydrogenase Expression in a Fumaric Acid Producing isolate Rhizopus oryzae 99-880 - (Peer Reviewed Journal)

Skory, C.D., Ibrahim, A.S. 2007. Native and modified lactate dehydrogenase expression in a fumaric acid producing isolate Rhizopus oryzae 99-880. Current Genetics. 52:23-33.

ISOLATION AND CHARACTERIZATION OF TWO GENES THAT ENCODE ACTIVE GLUCOAMYLASE WITHOUT A STARCH BINDING DOMAIN FROM A TYPE II RHIZOPUS ORYZAE - (Peer Reviewed Journal)

Mertens, J.A., Skory, C.D. 2007. Isolation and characterization of two genes that encode active glucoamylase without a starch binding domain from Rhizopus oryzae. Current Microbiology. 54:462-466.

PRODUCTION OF HIGHLY ACTIVE LIGNOCELLULOSE-DEGRADING ENZYMES OF ANAEROBIC FUNGI BY INDUSTRIALLY RELEVANT FUNGI - (Abstract)

Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Production of highly active lignocellulose-degrading enzymes of anaerobic fungi by industrially relevant fungi [abstract].

EXPRESSION OF AN AT-RICH XYLANASE GENE FROM THE ANAEROBIC FUNGUS ORPINOMYCES SP. STRAIN PC-2 IN AND SECRETION OF THE HETEROLOGOUS ENZYME BY HYPOCREA JECORINA - (Peer Reviewed Journal)

Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorina. Applied Microbiology and Biotechnology. 74:1264-1275.

EXPRESSION AND CHARACTERIZATION OF ACIDOTHERMUS CELLULOLYTICUS E1 ENDOGLUCANASE IN TRANSGENIC DUCKWEED LEMNA MINOR 8627 - (Peer Reviewed Journal)

Sun, Y., Cheng, J.J., Himmel, M.E., Skory, C.D., Adney, W.S., Thomas, S.R., Tisserat, B., Nishimura, Y., Yamamoto, Y.T. 2006. Expression and characterization of Acidothermus cellulolyticus E1 endoglucanase in transgenic duckweed Lemna minor 8627. Bioresource Technology. Available: http://www.sciencedirect.com, doi:10.1016/j.biortech.2006.09.055

ISOLATION AND CHARACTERIZATION OF A SECOND GLUCOAMYLASE WITHOUT A STARCH BINDING DOMAIN FROM RHIZOPUS ORYZAE - (Peer Reviewed Journal)

Mertens, J.A., Skory, C.D. 2007. Isolation and characterization of a second glucoamylase gene without a starch binding domain from Rhizopus oryzae. Enzyme and Microbial Technology. 40:874-880.

PLASMIDS FOR EXPRESSION OF HETEROLOGOUS PROTEINS IN RHIZOPUS ORYZAE - (Peer Reviewed Journal)

Mertens, J.A., Skory, C.D., Ibrahim, A.S. 2006. Plasmids for expression of heterologous proteins in Rhizopus oryzae. Archives of Microbiology. 186:41-50.

FUNCTIONAL PROTEOMIC PLASMID-BASED INTEGRATED WORKCELL FOR HIGH-THROUGHPUT TRANSFORMATION OF BL21 DE3 E. COLI FOR EXPRESSION IN VIVO WITH PIROMYCES STRAIN XYLOSE ISOMERASE - (Abstract)

Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Liu, S., Qureshi, N., Cotta, M.A., Skory, C.D., Gorsich, S.W., Farrelly, P.J. 2006. Functional proteomic plasmid-based integrated workcell for high-throughput transformation of BL21 DE3 E. coli for expression in vivo with piromyces strain xylose isomerase [abstract]. Midwest Laboratory Robotics Information Group. p. 2.

GENETIC MANIPULATION OF ZYGOMYCETES - (Book/Chapter)

Ibrahim, A.S., Skory, C.D. 2006. Genetic manipulation of zygomycetes. In: Kavanagh, K. editor. Medical Mycology, Cellular and Molecular Techniques. New York, NY: John Wiley & Sons. p. 305-325.

DEVELOPMENT OF PLASMIDS FOR EXPRESSION OF HETEROLOGOUS PROTEINS IN RHIZOPUS ORYZAE - (Abstract)

Mertens, J.A., Skory, C.D. 2005. Development of plasmids for expression of heterologous proteins in Rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 100.

TOLERANCE TO FURFURAL-INDUCED STRESS IS ASSOCIATED WITH PENTOSE PHOSPHATE PATHWAY GENES ZWF1, GND1, RPE1,AND TKL1 IN SACCHAROMYCES CEREVISIAE - (Peer Reviewed Journal)

Gorsich, S.W., Dien, B.S., Nichols, N.N., Slininger, P.J., Liu, Z., Skory, C.D. 2005. Tolerance to furfural-induced stress is associated with pentose phosphate pathway genes ZWF1, GND1, RPEL, and TKL1 in Saccharomyces cerevisiae. Applied Microbiology and Biotechnology. 71(3):339-349.

INHIBITION OF NON-HOMOLOGOUS END JOINING AND INTEGRATION OF DNA WITH TRANSFORMATION OF RHIZOPUS ORYZAE - (Peer Reviewed Journal)

Skory, C.D. 2005. Inhibition of non-homologous end joining and integration of DNA with transformation of Rhizopus oryzae. Molecular Genetics and Genomics. 274(4):373-83.

CONVERTING HERBACEOUS ENERGY CROPS TO BIOETHANOL: A REVIEW WITH EMPHASIS ON PRETREATMENT PROCESSES - (Book/Chapter)

Dien, B.S., Iten, L.B., Skory, C.D. 2005. Converting herbaceous energy crops to bioethanol: a review with emphasis on pretreatment processes. In: Hou, C.T., editor. Handbook of Industrial Biocatalysis. Chapter 23. Boca Raton, FL: Taylor & Francis Group. p. 1-11.

RECOMBINANT BIOCATALYSTS FOR CONVERTING SUGAR MIXTURES TO LACTIC ACID - (Abstract)

Dien, B.S., Whitehead, T.R., Nichols, N.N., Skory, C.D., Cotta, M.A. 2004. Recombinant biocatalysts for converting sugar mixtures to lactic acid [abstract]. Great Lakes Regional American Chemical Society Symposium. Paper No. 40.

DISCOVERY AND CHARACTERIZATION OF A SECOND GLUCOAMYLASE GENE IN RHIZOPUS ORYZAE - (Abstract)

Mertens, J.A., Skory, C.D. 2004. Discovery and characterization of a second glucoamylase gene in rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 96.

SILENCING OF LACTATE DEHYDROGENASE GENE OF RHIZOPUS ORYZAE - (Abstract)

Skory, C.D. 2004. Silencing of lactate dehydrogenase gene of Rhizopus oryzae [abstract]. American Society for Microbiology. p. 79.

REPAIR OF PLASMID DNA USED FOR TRANSFORMATION OF RHIZOPUS ORYZAE BY SYNTHESIS DEPENDENT STRAND ANNEALING - (Peer Reviewed Journal)

Skory, C.D. 2004. Repair of plasmid dna used for transformation of rhizopus oryzae by synthesis dependent strand annealing. Current Genetics. 45:302-310.

LACTIC ACID PRODUCTION BY RHIZOPUS ORYZAE WITH INCREASED LACTATE DEHYDROGENASE - (Peer Reviewed Journal)

Skory, C.D. 2004. Lactic acid production by Rhizopus oryzae transformants with lactate dehydrogenase activity. Applied Microbiology and Biotechnology. 64:237-242.

MUTATION REPAIR BY SYNTHESIS DEPENDENT STRAND ANNEALING IN RHIZOPUS ORYZAE - (Abstract)

Skory, C.D. 2003. Mutation repair by synthesis dependent strand annealing in Rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 97.

TRANSFORMATION OF ENZYME-TREATED RHIZOPUS ORYZAE GERMLINGS BY SQUARE-WAVE ELECTROPORATION - (Abstract)

RAI, G., RAYAPATI, J., TONUKARI, J., SKORY, C.D. TRANSFORMATION OF ENZYME-TREATED RHIZOPUS ORYZAE GERMLINGS BY SQUARE-WAVE ELECTROPORATION. FUNGAL GENETICS CONFERENCE. 2003. ABSTRACT NO. 467.

HOMOLOGOUS RECOMBINATION AND DOUBLE-STRAND BREAK REPAIR WITH TRANSFORMATION OF RHIZOPUS ORYZAE - (Peer Reviewed Journal)

SKORY, C.D. HOMOLOGOUS RECOMBINATION AND DOUBLE-STRAND BREAK REPAIR IN THE TRANSFORMATION OF RHIZOPUS ORYZAE. MOLECULAR GENETICS AND GENOMICS. 2002. V. 268. P. 397-406.

LACTIC ACID PRODUCTION BY SACCHAROMYCES CEREVISIAE EXPRESSING A RHIZOPUS ORYZAE LACTATE DEHYDROGENASE GENE - (Peer Reviewed Journal)

SKORY, C.D. LACTIC ACID PRODUCTION BY SACCHAROMYCES CEREVISIAE EXPRESSING A RHIZOPUS ORYZAE LACTATE DEHYDROGENASE GENE. JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY. 2003. V. 30. P. 22-27.

EXPRESSION OF ENDOGLUCANASE E1 IN TRANSGENIC DUCKWEED LEMNA MINOR - (Abstract)

SUN, Y., ADNEY, W.S., BERGMANN, B.A., CHENG, J., DECKER, S.R., FREER, S.N., HIMMEL, M.E., NISHIMURA, Y., SKORY, C.D., TISSERAT, B. EXPRESSION OF ENDOGLUCANASE E1 IN TRANSGENIC DUCKWEED LEMNA MINOR. BIOTECHNOLOGY FOR FUELS AND CHEMICALS SYMPOSIUM. 2002. ABSTRACT NO. 6-42.