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Application of Anaerobic and Multiple-Electron-Acceptor Bioremediation to Chlorinated Aliphatic Subsurface Contamination

EPA Grant Number: R825549C053
Subproject: this is subproject number 053 , established and managed by the Center Director under grant R825549
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: HSRC (1989) - Great Plains/Rocky Mountain HSRC
Center Director: Erickson, Larry E.
Title: Application of Anaerobic and Multiple-Electron-Acceptor Bioremediation to Chlorinated Aliphatic Subsurface Contamination
Investigators: Parkin, G. F.
Institution: University of Iowa
EPA Project Officer: Manty, Dale
Project Period: November 1, 1993 through June 2, 2000
Project Amount: Refer to main center abstract for funding details.
RFA: Hazardous Substance Research Centers - HSRC (1989)
Research Category: Organic Chemical Contamination of Soil/Water

Description:

Objective:

The goal of this project was to advance understanding of anaerobic and mixed-electron-acceptor bioremediation of chlorinated aliphatics to a level that full-scale evaluation of these processes is possible.

Approach:

A variety of enrichment cultures were developed as a part of this project. Anaerobic enrichments were maintained on lactate or acetate and a basic inorganic, buffered nutrient medium, while an aerobic methanotrophic enrichment culture was developed for sequential anaerobic-aerobic column studies. All enrichments and experiments were maintained at 20°C. Experiments assessing the effect of mixtures of CT, PCE, and 1,1,1-TCA were conducted primarily in 38-mL serum bottles. Sequential anaerobic-aerobic reactors were glass columns with 1.5-inch O.D. and 12 inches long, connected in series. All were filled with 3-mm borosilicate glass beads except for one anaerobic column filled with steel wool.

Compounds were analyzed by a variety of techniques (headspace analysis by GC for volatile compounds such as the chlorinated compounds, ethene, ethane, methane and H2; HPLC analysis for non-volatile compounds such as lactate; IC analysis for ionized compounds such as nitrate, acetate, and perchlorate).

Expected Results:

This research focuses on three chlorinated aliphatics that are not degraded (or are degraded very slowly) by aerobic bacteria: perchloroethene (PCE), 1,1,1-trichloroethane (1,1,1 TCA), and carbon tetrachloride (CT). If successful, field-scale evaluation of technologies developed in this research will be pursued.

Publications and Presentations:

Publications have been submitted on this subproject: View all 28 publications for this subproject | View all 900 publications for this center

Journal Articles:

Journal Articles have been submitted on this subproject: View all 7 journal articles for this subproject | View all 188 journal articles for this center

Supplemental Keywords:

anaerobic, bioremediation, chlorinated aliphatics, mixed-electron acceptor. , Ecosystem Protection/Environmental Exposure & Risk, Toxics, Scientific Discipline, Waste, Analytical Chemistry, Fate & Transport, Environmental Chemistry, Contaminated Sediments, 33/50, Ecology and Ecosystems, Geochemistry, Bioremediation, electron acceptors, 1, 1, 1-Trichloroethane, biodegradation, fate and transport, humification, biotechnology, adsorption, chemical kinetics, contaminated sediment, contaminant transport, carbon tetrachloride, contaminants in soil, contaminated soils, bioremediation of soils

Progress and Final Reports:
Final Report


Main Center Abstract and Reports:
R825549    HSRC (1989) - Great Plains/Rocky Mountain HSRC

Subprojects under this Center: (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R825549C006 Fate of Trichloroethylene (TCE) in Plant/Soil Systems
R825549C007 Experimental Study of Stabilization/Solidification of Hazardous Wastes
R825549C008 Modeling Dissolved Oxygen, Nitrate and Pesticide Contamination in the Subsurface Environment
R825549C009 Vadose Zone Decontamination by Air Venting
R825549C010 Thermochemical Treatment of Hazardous Wastes
R825549C011 Development, Characterization and Evaluation of Adsorbent Regeneration Processes for Treament of Hazardous Waste
R825549C012 Computer Method to Estimate Safe Level Water Quality Concentrations for Organic Chemicals
R825549C013 Removal of Nitrogenous Pesticides from Rural Well-Water Supplies by Enzymatic Ozonation Process
R825549C014 The Characterization and Treatment of Hazardous Materials from Metal/Mineral Processing Wastes
R825549C015 Adsorption of Hazardous Substances onto Soil Constituents
R825549C016 Reclamation of Metal and Mining Contaminated Superfund Sites using Sewage Sludge/Fly Ash Amendment
R825549C017 Metal Recovery and Reuse Using an Integrated Vermiculite Ion Exchange - Acid Recovery System
R825549C018 Removal of Heavy Metals from Hazardous Wastes by Protein Complexation for their Ultimate Recovery and Reuse
R825549C019 Development of In-situ Biodegradation Technology
R825549C020 Migration and Biodegradation of Pentachlorophenol in Soil Environment
R825549C021 Deep-Rooted Poplar Trees as an Innovative Treatment Technology for Pesticide and Toxic Organics Removal from Soil and Groundwater
R825549C022 In-situ Soil and Aquifer Decontaminaiton using Hydrogen Peroxide and Fenton's Reagent
R825549C023 Simulation of Three-Dimensional Transport of Hazardous Chemicals in Heterogeneous Soil Cores Using X-ray Computed Tomography
R825549C024 The Response of Natural Groundwater Bacteria to Groundwater Contamination by Gasoline in a Karst Region
R825549C025 An Electrochemical Method for Acid Mine Drainage Remediation and Metals Recovery
R825549C026 Sulfide Size and Morphology Identificaiton for Remediation of Acid Producing Mine Wastes
R825549C027 Heavy Metals Removal from Dilute Aqueous Solutions using Biopolymers
R825549C028 Neutron Activation Analysis for Heavy Metal Contaminants in the Environment
R825549C029 Reducing Heavy Metal Availability to Perennial Grasses and Row-Crops Grown on Contaminated Soils and Mine Spoils
R825549C030 Alachlor and Atrazine Losses from Runoff and Erosion in the Blue River Basin
R825549C031 Biodetoxification of Mixed Solid and Hazardous Wastes by Staged Anaerobic Fermentation Conducted at Separate Redox and pH Environments
R825549C032 Time Dependent Movement of Dioxin and Related Compounds in Soil
R825549C033 Impact of Soil Microflora on Revegetation Efforts in Southeast Kansas
R825549C034 Modeling the use of Plants in Remediation of Soil and Groundwater Contaminated by Hazardous Organic Substances
R825549C035 Development of Electrochemical Processes for Improved Treatment of Lead Wastes
R825549C036 Innovative Treatment and Bank Stabilization of Metals-Contaminated Soils and Tailings along Whitewood Creek, South Dakota
R825549C037 Formation and Transformation of Pesticide Degradation Products Under Various Electron Acceptor Conditions
R825549C038 The Effect of Redox Conditions on Transformations of Carbon Tetrachloride
R825549C039 Remediation of Soil Contaminated with an Organic Phase
R825549C040 Intelligent Process Design and Control for the Minimization of Waste Production and Treatment of Hazardous Waste
R825549C041 Heavy Metals Removal from Contaminated Water Solutions
R825549C042 Metals Soil Pollution and Vegetative Remediation
R825549C043 Fate and Transport of Munitions Residues in Contaminated Soil
R825549C044 The Role of Metallic Iron in the Biotransformation of Chlorinated Xenobiotics
R825549C045 Use of Vegetation to Enhance Bioremediation of Surface Soils Contaminated with Pesticide Wastes
R825549C046 Fate and Transport of Heavy Metals and Radionuclides in Soil: The Impacts of Vegetation
R825549C047 Vegetative Interceptor Zones for Containment of Heavy Metal Pollutants
R825549C048 Acid-Producing Metalliferous Waste Reclamation by Material Reprocessing and Vegetative Stabilization
R825549C049 Laboratory and Field Evaluation of Upward Mobilization and Photodegradation of Polychlorinated Dibenzo-P-Dioxins and Furans in Soil
R825549C050 Evaluation of Biosparging Performance and Process Fundamentals for Site Remediation
R825549C051 Field Scale Bioremediation: Relationship of Parent Compound Disappearance to Humification, Mineralization, Leaching, Volatilization of Transformaiton Intermediates
R825549C052 Chelating Extraction of Heavy Metals from Contaminated Soils
R825549C053 Application of Anaerobic and Multiple-Electron-Acceptor Bioremediation to Chlorinated Aliphatic Subsurface Contamination
R825549C054 Application of PGNAA Remote Sensing Methods to Real-Time, Non-Intrusive Determination of Contaminant Profiles in Soils
R825549C055 Design and Development of an Innovative Industrial Scale Process to Economically Treat Waste Zinc Residues
R825549C056 Remediation of Soils Contaminated with Wood-Treatment Chemicals (PCP and Creosote)
R825549C057 Effects of Surfactants on the Bioavailability and Biodegradation of Contaminants in Soils
R825549C058 Contaminant Binding to the Humin Fraction of Soil Organic Matter
R825549C059 Identifying Ground-Water Threats from Improperly Abandoned Boreholes
R825549C060 Uptake of BTEX Compounds by Hybrid Poplar Trees in Hazardous Waste Remediation
R825549C061 Biofilm Barriers for Waste Containment
R825549C062 Plant Assisted Remediation of Soil and Groundwater Contaminated by Hazardous Organic Substances: Experimental and Modeling Studies
R825549C063 Extension of Laboratory Validated Treatment and Remediation Technologies to Field Problems in Aquifer Soil and Water Contamination by Organic Waste Chemicals

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The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Conclusions drawn by the principal investigators have not been reviewed by the Agency.

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