Project Number: 6645-21000-026-27
Project Type: Specific Cooperative Agreement

Start Date: Apr 21, 2008
End Date: Feb 28, 2009

Objective:
To identify mutant and new naturally occurring alleles that enhance oleic acid content by screening for GmFAD2-1A and GmFAD2-1B gene mutations in an EMS mutagenized population of the cultivar Forrest and in 132 accessions of the germplasm collection reported to have elevated oleate phenotypes. To continue candidate gene analysis and marker development of mutant soybean germplasm displaying additional novel fatty acid phenotypes.

Approach:
Plant introductions with putative elevated oleic acid will be tested in replicated field trials to determine the actual level of oleic acid. FAD2 genes in confirmed high oleic materials will be characterized. FAD2 mutants from an EMS mutagenized population will be characterized by DNA analysis and phenotyped in field trials. Gene mutations responsible for other novel soybean oil traits will be defined by sequencing candidate genes.