Project Number: 6612-32000-049-02
Project Type: Nonfunded Cooperative Agreement

Start Date: Sep 12, 2007
End Date: Apr 01, 2012

Objective:
The objective of this cooperative research project is to develop new fluorescent-based viral detection reagents for the study of Newcastle disease virus.

Approach:
Fluorescent molecular beacon-based probes targeted to the viral RNA will be used to analyse Newcastle Disease Virus replication. This approach allows for the analysis of the basic replication and assembly processes in four dimensions; involving space (x,y,z) and time. Initial studies will focus on the use of vaccine strain of NDV investigated in chicken embryo fibroblasts, while future studies will progress to mesogenic and velogenic strains for comparison.