Projects

GENETIC AND BIOLOGICALLY-BASED MANAGEMENT OF VEGETABLE CROP DISEASES Appropriated (D)   Accession Number: 411055

DEVELOP REAL-TIME PCR FOR DETECTION OF DIDYMELLA BRYONIAE ISOLATES Nonfunded Cooperative Agreement (N)   Accession Number: 409777

Publications

Pepino Mosaic Virus on Tomato Seed: Virus Location and Mechanical Transmission - (Peer Reviewed Journal) - (09-Sep-08)

Genetic Variability and Population Structure of Pepino Mosaic Virus in North-American Greenhouse Tomatoes - (Peer Reviewed Journal) - (02-Sep-08)

Black Tea and Lignin as Ultraviolet Protectants for the Beet Armyworm Nucleopolyhedrovirus - (Peer Reviewed Journal) - (27-Jun-08)

Forty-nine New Host Plant Species of Bemisia tabaci (Hemiptera: Aleyrodidae) - (Peer Reviewed Journal) - (12-Jun-08)

CAPS Markers in an eIF4E Gene are Linked to Zucchini Yellow Mosaic Virus Resistant Locus in Watermelon - (Abstract)

Ling, K., Harris, K.R., Meyer, J.D., Levi, A., Guner, N., Wehner, T.C., Havey, M.J. 2008. Caps markers in an eif4e gene are linked to zucchini yellow mosaic virus resistant locus in watermelon. Phytopathology. 98(6 supplement):S91.

Development of a Multiplex Real-Time PCR Assay for the Simultaneous Detection of Three Seedborne Pathogen Types in Cucurbits - (Abstract)

Ling, K., Wechter, W.P., Walcott, R.R., Keinath, A.P. 2008. Development of a Multiplex Real-Time PCR Assay for the Simultaneous Detection of Three Seedborne Pathogen Types in Cucurbits. Phytopathology. 98(6 supplement):S91.

Development of an Improved Real-Time PCR System for Broad Spectrum Detection of Diverse Didymella Bryoniae Genotypes - (Abstract)

Ling, K., Wechter, W.P., Somai, B.M., Walcott, R.R., Keinath, A.P. 2008. Development of an Improved Real-Time PCR System for Broad Spectrum Detection of Diverse Didymella Bryoniae Genotypes. Phytopathology. 98(6 supplement):S91.

Identification of a CAPS Marker in an eIF4E Gene Linked to Zucchini Yellow Mosaic Virus Resistant Locus in Watermelon - (Proceedings/Symposium)

Ling, K., Harris, K.R., Meyer, J.D., Levi, A., Guner, N., Wehner, T.C., Havey, M.J. 2008. Identification of a CAPS Marker in an eIF4E Gene Linked to Zucchini Yellow Mosaic Virus Resistant Locus in Watermelon. Cucurbitaceae Proceedings. 1Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae, (Ptrat M., ed), INRA, Avignon (France): 213-218.

Potential Sources of Resistance to Cucurbit Powdery Mildew (Podosphaera xanthii) in US Plant Introductions (PI) of Lagenaria Siceraria (bottle gourd) - (Peer Reviewed Journal)

Kousik, C.S., Levi, A., Ling, K., Wechter, W.P. 2008. Potential Sources of Resistance to Cucurbit Powdery Mildew (Podosphaera xanthii) in US Plant Introductions (PI) of Lagenaria Siceraria (bottle gourd). HortScience. 43(5):1359-1364

Whitefly Transmission of the Sweet Potato Leaf Curl Virus - (Abstract) - (15-Mar-08)

First Report of Southern Blight on Bottle Gourd (Lagenaria siceraria) caused by Sclerotium Rolfsii in South Carolina - (Peer Reviewed Journal)

Ling, K., Kousik, C.S., Keinath, A.P. 2008. First Report of Southern Blight on Bottle Gourd (Lagenaria siceraria) caused by Sclerotium Rolfsii in South Carolina. Plant Disease. 92:656.

Efficient Regeneration and Selection of Virus-free Sweetpotato Plants from Sweet Potato Leaf Curl Virus Infected Materials and Their Effects on Yields in Field Trials - (Abstract)

Ling, K., Jackson, D.M., Harrison Jr, H.F., Pesic-Vanesbroeck, Z., Hoy, M., Clark, C.A. 2008. Efficient Regeneration and Selection of Virus-free Sweetpotato Plants from Sweet Potato Leaf Curl Virus Infected Materials and Their Effects on Yields in Field Trials. Meeting Abstract. National Sweetpotato Collaborators Group Progress Report 2007. P7.

Sweet Potato Leaf Curl Virus: Virus Reservoir in Species of Wild Morning Glory - (Abstract)

Ling, K., Harrison Jr, H.F., Simmons, A.M., Jackson, D.M. 2008. Sweet Potato Leaf Curl Virus: Virus Reservoir in Species of Wild Morning Glory. Meeting Abstract. National Sweetpotato Collaborators Group Progress Report 2007. P8.

Sweet Potato Leaf Curl Virus: Efficiency of Whitefly Transmission - (Abstract)

Simmons, A.M., Ling, K., Harrison Jr, H.F., Jackson, D.M. 2008. Sweet Potato Leaf Curl Virus: Efficiency of Whitefly Transmission. National Sweetpotato Collaborators Group Progress Report 2007. P6.

EST-SSRs of Watermelon (Citrullus sp.) Useful in Assessing Genetic Diversity among Lagenaria Siceraria Accessions - (Abstract)

Levi, A., Ling, K., Davis, A.R. 2008. Est-ssrs of watermelon (citrullus sp.) useful in assessing genetic diversity among lagenaria siceraria accessions. Plant and Animal Genome XVI Conference, P643.

Resistance to Grapevine Leafroll Associated Virus-2 is Conferred by Post-Transcriptional Gene Silencing in Transgenic Nicotiana benthamiana - (Peer Reviewed Journal)

Ling, K., Zhu, H., Gonsalves, D. 2008. Resistance to Grapevine Leafroll Associated Virus-2 is Conferred by Post-Transcriptional Gene Silencing in Transgenic Nicotiana benthamiana. Transgenic Research. 17:733-740.

Tolerance to Cucurbit Powdery Mildew in USDA Bottle Gourd (Lagenaria siceraria) Plant Introductions (PI) - (Abstract)

Kousik, C.S., Ling, K., Levi, A. 2007. Tolerance to Cucurbit Powdery Mildew in USDA Bottle Gourd (Lagenaria siceraria) Plant Introductions (PI). Phytopathology. 97:S60.

The Population Genetics of Pepino Mosaic Virus in North America Greenhouse Tomatoes - (Abstract)

Ling, K. 2007. The Population Genetics of Pepino Mosaic Virus in North America Greenhouse Tomatoes. Phytopathology. 97:S65.

Development of a One-Step Immunocapture Real-Time TaqMan RT-PCR Assay for the Broad Spectrum Detection of Pepino Mosaic Virus - (Peer Reviewed Journal)

Ling, K., Wechter, W.P., Jordan, R.L. 2007. Development of a One-Step Immunocapture Real-Time TaqMan RT-PCR Assay for the Broad Spectrum Detection of Pepino Mosaic Virus. Journal of Virological Methods 144:65-72.

Interspecific Transferability of Watermelon EST-SSR Markers in Cucurbit Species - (Abstract)

Levi, A., Wechter, W.P., Davis, A.R., Katzir, N., Tadmor, K., Ling, K., Reddy, U. 2007. Interspecific Transferability of Watermelon EST-SSR Markers in Cucurbit Species. HortScience. 42:1012.

Sources of Resistance to Zucchini Yellow Mosaic Virus in Lagenaria Siceraria Germplasm - (Peer Reviewed Journal)

Ling, K., Levi, A. 2007. Sources of Resistance to Zucchini Yellow Mosaic Virus in Lagenaria Siceraria Germplasm. HortScience. 42(5):1124-1126.

Sources of Resistance to Pepino Mosaic Virus in Tomato Accessions - (Peer Reviewed Journal)

Ling, K., Scott, J.W. 2007. Sources of Resistance to Pepino Mosaic Virus in Tomato Accessions. Plant Disease. 91:749-753.

Regenerating Virus-free Materials for USDA Heirloom Sweetpotato Cultivar Collection through the Meristem Shoot-tip Culture Technology - (Abstract)

Ling, K., Hoy, M., Bohac, J., Jackson, D.M., Clark, C.A. 2007. Regenerating Virus-free Materials for USDA Heirloom Sweetpotato Cultivar Collection through the Meristem Shoot-tip Culture Technology. Meeting Abstract. National Sweetpotato Collaborators Group Progress Report, 2006. p. 4.

Characterization, Diagnosis & Management of Plant Viruses, Vol. 2: Horticultural Crops - (Book/Chapter)

Rao, G.P., Myrta, A., Ling, K. 2008. Characterization, Diagnosis & Management of Plant Viruses, Vol. 2: Horticultural Crops. Complete Book.

Wild Germplasm: Plant Resistance for Watermelon - (Abstract)

Simmons, A.M., Levi, A., Davis, A.R., Ling, K., Lopez, R., Jackson, D.M., Kousik, C.S., Shepard, B.M., Thies, J.A. 2006. Wild Germplasm: Plant Resistance for Watermelon. Bemisia International Workshop Proceedings, Duck Key, FL. pp 130.

SENSITIVE DETECTION OF GRAPEVINE LEAFROLL VIRUS-2 WITH ELISA USING AN ANTISERUM AGAINST THE RECOMBINANT COAT PROTEIN - (Peer Reviewed Journal)

Ling, K., Zhu, H., Petrovic, N., Gonsalves, D. 2007. Sensitive Detection of Grapevine Leafroll Virus-2 with Elisa Using an Antiserum Against the Recombinant Coat Protein. Journal of Phytopathology. (2007) 155:65-69.

TWO VARIANTS OF PEPINO MOSAIC VIRUS ISOLATED FROM IMPORTED TOMATO SEED FROM CHILE SHARE HIGH LEVELS OF SEQUENCE IDENTITY WITH THE US ISOLATES - (Abstract)

Ling, K. 2006. Two variants of pepino mosaic virus isolated from imported tomato seed from chile share high levels of sequence identity with the us isolates. Meeting Abstract. Phytopathology 96:S69.

EVALUATING ZUCCHINI YELLOW MOSAIC VIRUS RESISTANCE IN WATERMELON - (Proceedings/Symposium)

Ling, K., Levi, A., Guner, N., Wehner, T.C. 2006. Evaluating Zucchini Yellow Mosaic Virus Resistance in Watermelon. Pages 468-472 in: Proceedings of Cucurbitaceae 2006. G. J. Holmes, ed. Universal Press, Raleigh, North Carolina.

SCREENING TOMATO GERMPLASM FOR RESISTANCE TO PEPINO MOSAIC VIRUS - (Abstract)

Ling, K., Scott, J.W. 2006. Screening tomato germplasm for resistance to pepino mosaic virus. Meeting Abstract. http://roundtable06.ifas.ufl.edu/abstracts.htm

MOLECULAR CHARACTERIZATION OF TWO PEPINO MOSAIC VIRUS ISOLATES FROM IMPORTED TOMATO SEED REVEALS HIGH LEVELS OF SEQUENCE IDENTITY BETWEEN CHILEAN AND US ISOLATES - (Peer Reviewed Journal)

Ling, K. Molecular Characterization of Two Pepino Mosaic Virus Isolates from Imported Tomato Seed Reveals High Levels of Sequence Identity between Chilean and US Isolates. Virus Genes. (2007)34:1-8.

Sources of Resistance to Pepino Mosaic Virus in Solanum habrochaites (Lycopersicon hirsutum) - (Proceedings/Symposium)

Ling, K., Scott, J.W. 2007. Sources of Resistance to Pepino Mosaic Virus in Solanum habrochaites (Lycopersicon hirsutum). http://www.ces.ncsu.edu/fletcher/programs/plantpath/2006-11-tomato-disease/index.html Proceedings of the 22nd Annual Tomato Disease Workshop, p 1-3.

AN UPDATE ON EVALUATION OF VIRUS STATUS IN THE HEIRLOOM SWEETPOTATO GERMPLASM MATERIALS WITH REAL-TIME PCR TECHNOLOGY - (Abstract)

Ling, K., Clark, C.A., Kokkinos, C., Bohac, J., Hurtt, S.S., Jarret, R.L., Gillapie, A.G. 2006. An update on evaluation of virus status in the heirloom sweetpotato germplasm materials with real-time pcr technology. HortScience 41:517.

NOVEL WATERMELON BREEDING LINES CONTAINING CHLOROPLAST AND MITOCHONDRIAL GENOMES OF THE DESERT SPECIES CITRULLUS COLOCYNTHIS - (Peer Reviewed Journal)

Levi, A., Thomas, C.E., Thies, J.A., Simmons, A.M., Ling, K., Harrison Jr, H.F. 2006. Novel watermelon breeding lines containing chloroplast and mitochondrial genomes of the desert species citrullus colocynthis. HortScience. 41:463-464.

FIRST REPORT OF TOMATO YELLOW LEAF CURL VIRUS IN SOUTH CAROLINA - (Peer Reviewed Journal)

Ling, K., Simmons, A.M., Hassell, R.L., Keinath, A.P., Polston, J.E. 2006. First report of tomato yellow leaf curl virus in south carolina. Plant Disease Series. 90:379.

Grapevine Leafroll Associated Viruses - (Book/Chapter)

Ling, K., Zhu, H., Gonsalves, D. 2008. Grapevine Leafroll Associated Viruses. Molecular Diagnosis of Plant Viruses. Horticultural Crops/Studium Press, Houston, Texas. pp 181-199.

REALTIME IC RT-PCR DETECTION OF PEPINO MOSAIC VIRUS ON TOMATO SEED AND PLANT TISSUES IN A SINGLE TUBE - (Abstract)

Ling, K. 2005. Realtime immunocapture RT-PCR detection of Pepino Mosaic virus on tomato seed and plant tissues in a single tube [abstract]. Phytopathology 95 (6):S61.

HIGH SEQUENCE HOMOLOGY BETWEEN PEPINO MOSAIC VIRUS ISOLATES FROM CHILE AND THE USA - (Abstract)

Ling, K. 2005. High sequence homology between pepino mosaic virus isolates from Chile and the USA [abstract]. Second Joint Conference of the International Working Groups on Legume (IWGLV) and Vegetable Viruses (IWGVV). p. 20 Available: http://www.ifa.to.cnr.it/vvwg/documents/Abstracts_Rev.pdf.

PROGRESS TOWARDS COMMERCIAL-SCALE DETECTION OF A. AVENAE SUBSP. CITRULLI IN CUCURBIT SEED USING IMMUNOMAGNETIC SEPARATION AND THE POLYMERASE CHAIN REACTION - (Peer Reviewed Journal)

Walcott, R.R., Castro, A.C., Fessehaie, A., Ling, K. 2006. Progress towards commercial-scale detection of a. avenae subsp. citrulli in cucurbit seed using immunomagnetic separation and the polymerase chain reaction. Seed Science and Technology. 34:101-116.

NOTICE OF RELEASE OF USVL-205 AND USVL-210, NOVEL WATERMELON BREEDING LINES - (Germplasm Release)

Levi, A., Thomas, C.E., Thies, J.A., Simmons, A.M., Ling, K., Hassell, R., Keinath, A.P. 2005. Notice of release of USVL-205 and USVL-210, novel watermelon breeding lines. USDA, Agricultural Research Service, Washington, D.C. Cultivar Release. February 15, 2005.

PEPINO MOSAIC VIRUS - (Review Article) - (17-Sep-04)

PEPINO MOSACI VIRUS, AN EMERGING DISEASE IN GREENHOUSE TOMATO PRODUCTION WORLDWIDE: IS SEED RESPONSIBLE? - (Proceedings/Symposium)

Ling, K., Carpenter, L. 2005. Pepino Mosaci Virus, An Emerging Disease in Greenhouse Tomato Production Worldwide: Is Seed Responsible?. Acta Horticulture. (ISHS) 695:43-50.

COMPLETE NUCLEOTIDE SEQUENCE AND GENOME ORGANIZATION OF GRAPEVINE LEAFROLL-ASSOCIATED VIRUS-3, TYPE MEMBER OF THE GENUS AMPELOVIRUS - (Peer Reviewed Journal)

Ling, K., Zhu, H., Gonsalves, D. 2004. Complete nucleotide sequence and genome organization of grapevine leafroll-associated virus-3, type member of the genus Ampelovirus. Journal of General Virology. 85:2099-2102.