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![](https://webarchive.library.unt.edu/eot2008/20081030181600im_/http://www.ars.usda.gov/incme/images/Research_head.gif) |
Research Project:
IDENTIFICATION AND CHARACTERIZATION OF GENES AFFECTING COOL AND COLD WATER AQUACULTURE PRODUCTION
Location: Leetown, West Virginia
Title: Genetic variations of 14-3-3E1 isoform in rainbow trout (Oncorhynchus mykiss)
Authors
![item](https://webarchive.library.unt.edu/eot2008/20081030181600im_/http://www.ars.usda.gov/incme/images/bullet.gif) | Wanna, Warapond - WEST VIRGINIA UNIVERSITY | ![item](https://webarchive.library.unt.edu/eot2008/20081030181600im_/http://www.ars.usda.gov/incme/images/bullet.gif) |
Rexroad, Caird
| ![item](https://webarchive.library.unt.edu/eot2008/20081030181600im_/http://www.ars.usda.gov/incme/images/bullet.gif) | Jianbo, Yao - WEST VIRGINIA UNIVERSTIY |
Submitted to: Plant and Animal Genome Conference
Publication Type:
Abstract
Publication Acceptance Date: January 12, 2008
Publication Date: January 12, 2008
Citation: Wanna, W., Rexroad III, C.E., Jianbo, Y. 2008. Genetic variations of 14-3-3E1 isoform in rainbow trout (Oncorhynchus mykiss). Plant and Animal Genome Conference.
Technical Abstract: The highly conserved family of 14-3-3 proteins functions in the regulation of a wide variety of cellular processes. The presence of 14-3-3 isoforms and the diversity of cellular processes regulated by 14-3-3 isoforms suggest functional specificity of the isoforms. Several studies have observed differences in affinity and functionality of 14-3-3 isoforms. However, the structural variation by which isoform specificity is accomplished remains unclear. In rainbow trout, ten 14-3-3 genes which are likely to be duplicates of five ancestral genes have been reported. In this study, we examined the genetic variations of the 14-3-3E1 gene in rainbow trout. The open reading frame (ORF) of the 14-3-3E1 gene was amplified from a pool of cDNAs derived from 10 different rainbow trout tissues and cloned into pCRII vector. A total of 14 random clones were sequenced. DNA sequence analysis showed substantial sequence variations in the ORF of the 14-3-3E1 gene. A number of missense mutations were identified that result in several single amino acid substitutions. Four of the clones showed a deletion of 3 nucleotides (CAG), resulting in the deletion of Alanine at position 239. Interesting, two sequences showed an insertion of 45 nucleotides that results in the introduction of a premature stop codon. This insertion mutation was confirmed by PCR detection of a larger fragment. The presence of numerous mutations in the coding region of the 14-3-3E1 gene is an important indication of the diverse functions of this protein.
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Last Modified: 10/29/2008
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