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OWML: QA/QC

Quality Assurance/Quality Control Manual: Ohio Water Microbiology Laboratory

 

Table 4.  Acceptance criteria for laboratory refrigerators, freezers, incubators, and waterbaths

Equipment identification

Use

Acceptance criteria

INC 2

Actinomycetes

28°C ± 1.0°C

INC 3

Hybridization oven

51°C ± 1.0°C, 80°C ± 1.0°C

INC 4

General use

36°C ± 1°C

INC 5

Method 1601/1602, transfer cultures, general use

36°C ± 1.0°C

INC 6

Method 1601/1602,  transfer cultures, general use

36°C ± 1.0°C

INC 7

Method 1601/1602,  transfer cultures, general use

36°C ± 1.0°C

W/B 1

Melt and temper agar

48°C ± 3.0°C

W/B 2

Method 1602, heat treatments

37°C ± 1.0°C, 70°C ± 3.0°C

W/B 3

Grow hosts/1602

36°C ± 1.0°C, 48°C ± 1.0°C

W/B 4

Shaking bath for hybridization

51°C ± 0.2°C

W/B 5

Heat treatments

70°C ± 3.0°C

W/B 6

Grow hosts/1602

36°C ± 1.0°C, 48°C ± 1.0°C

REFRIG 1

Sample storage

1 to 4°C

REFRIG 2

Sample storage

1 to 4°C

REFRIG 3

Reagent storage

1 to 4°C

REFRIG 4

Sample storage

1 to 4°C

REFRIG 5

Reagent/ media storage

1 to 4°C

FREEZ 1

Reagent storage

 -20°C to -30°C

FREEZ 2

Ice packs, biological sample storage

 -20°C to -30°C

FREEZ 3

Bacteria stocks, virus stocks, samples

Shelf 1 -70°C ± 10°C

Shelf 2 -70°C ± 10°C

Shelf 3 -60°C ± 10°C

Shelf 4 -60°C ± 10°C

Shelf 5 -60°C ± 10°C

FREEZ 4

Sample storage

-70°C ± 10°C

FREEZ 5

Probes, hybridization reagents

 -20°C to -30°C

FIELD INCUBATORS

Membrane filtration

35°C ± 1.0°C, 44.5°C ± 0.5°C, 41.5°C ± 0.5°C

 

   

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