Ohio Water Microbiology Lab
Research Topics
Internal Information
Ohio Water Science Center
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OWML: QA/QC
Quality Assurance/Quality Control Manual: Ohio Water Microbiology
Laboratory
Sample Management and Documentation
Samples for the bacterial indicators, E. coli, enterococci, fecal coliform, and
total coliforms, are most commonly processed and analyzed in the field; however,
they may be done in the Ohio Water Microbiology Laboratory (OWML). Holding times
are 6 hours for compliance purposes and 24 hours for noncompliance purposes
(American Public Health Association, 1998, Section 9060 B.) Adhering to a 6-hour
holding time for all bacteriological samples, however, is highly recommended.
Samples for Clostridium perfringens and coliphage are processed and analyzed in
the OWML; samples are kept on ice and processed within 48 hours of sample
collection. Samples for the analysis of coliphage that arrive chilled within 48
to 96 hours from collection are acceptable, but the results are qualified.
Samples for Cryptosporidium are processed in the OWML within 48 hours of sample
collection. Samples for enteric viruses arrive at the OWML concentrated on
filters; they must be kept on ice and processed within 24 hours of filtration.
Samples for Actinomycetes are processed in the OWML; samples are kept on ice and
processed within 24 hours of sample collection. Samples for bacterial markers
generally arrive at the OWML as whole water samples; these samples are filtered
and frozen within 24 hours of sample collection.
All requests for laboratory analyses must be submitted using a service request
form (Appendix
A). The following categories must be filled out when requesting sample
analysis: station name, site number, date/time of sample collection, medium
code, sample type, Water Center user code, and project number. The field
personnel must check off requested analyses and make prior arrangements with
laboratory personnel. Upon receipt of the sample in the laboratory, personnel
will fill in Received By and Date Received on the front of the Service Request
form.
Laboratory personnel will then enter sample information on to a log sheet (Appendix
B) and log the sample into LIMS, which will assign a login ID. There is one
sample logbook that contains a log sheet, service request forms, and results
bench sheets. Laboratory personnel will write the login ID on the Service
Request form (front and back) and on the sample bottle (or filter cartridge).
The Service Request form is filed in the sample logbook. Samples are stored in
the laboratory refrigerators until processing. Appropriate bench sheets are
routed to the analyst, who will enter sample login ID, processing date/times,
and analytical information.
Upon completion of the analysis, the analyst writes final results on the back of
the Service Request form. A second analyst routinely checks the calculations of
the analyst performing the work. The results are entered into the LIMS. All data
supported by NWIS will be sent to the Water Quality Data Transfer System (QWDX)
and available for download at
https://qwdx.cr.usgs.gov/. An email will be sent to all clients when the
data is available for download. Other data will be sent directly to the client.
After all of the sample result information is distributed to the client, the
Service Request form and appropriate bench sheets are then filed together in an
archived folder sorted by login ID. |