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Aerobic Cometabolism of Methyl tert-butyl Ether by Microorganisms Grown on Aliphatic Hydrocarbons
EPA Grant Number: R825689C029Subproject: this is subproject number 029 , established and managed by the Center Director under grant R825689
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
Center: HSRC (1989) - Western HSRC
Center Director: McCarty, Perry L.
Title: Aerobic Cometabolism of Methyl tert-butyl Ether by Microorganisms Grown on Aliphatic Hydrocarbons
Investigators: Williamson, Kenneth J. , Ciuffetti, Lynda
Institution: Oregon State University
EPA Project Officer: Manty, Dale
Project Period: January 1, 1999 through January 1, 2000
Project Amount: Refer to main center abstract for funding details.
RFA: Hazardous Substance Research Centers - HSRC (1989)
Research Category: Hazardous Substance Research Centers
Description:
Objective:To advance the use of cometabolic bioremediation for the removal of MTBE from contaminated groundwaters. Approach:
MTBE has been found to be a wide contaminant in groundwater as a result of gasoline spills and leaking underground storage tanks. Remediation technologies are desperately needed by regulatory personnel to facilitate the cleanup of contaminated groundwater at reasonable costs. Unfortunately, both conventional pump-and-treat technologies and soil vapor extraction lack effectiveness for removal of MTBE. While unproven, bioremediation by cometabolic organisms appears to be a promising technology to achieve removal of MTBE.
The approach will include:
All experiment will include mass balances of MTBE, the primary substrate
(propane or isopentane), cell protein, and dissolved oxygen.
Groundwater, MTBE, biodegradation, bioremediation, cometabolism, hazardous waste. , Scientific Discipline, Waste, RFA, Remediation, Biology, Microbiology, Chemistry, Hazardous Waste, Biochemistry, Environmental Engineering, Environmental Microbiology, Environmental Chemistry, Groundwater remediation, Hazardous, Bioremediation, Engineering, gasoline, biodegradation, aerobic cometabolism, Methyl tert butyl ether, MTBE, groundwater, contaminated aquifers, mycobacterium vaccae, in-situ bioremediation, cometabolism, groundwater contamination, in situ treatment, contaminated groundwater, microflora, degradation rates, filamentous fungus, graphium sp.
Progress and Final Reports:
Final Report
Main Center Abstract and Reports:
R825689 HSRC (1989) - Western HSRC
Subprojects under this Center:
(EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).
R825689C001 Interactions between Electron Acceptors in the Treatment
of Wastewaters Containing Sulfate, Chlorophenols and Acetate
R825689C002 Enhancing Biodegradation with Sorption and Alternating
Aerobic/Anaerobic Environments
R825689C003 Development and Verification of a Numerical Model to Predict
the Fate and Transport of Chlorinated Phenols in Groundwater
R825689C004 Redox Transformations of Inorganic Pollutants: Coupling
to the Biogeochemical Matrix
R825689C005 Hexavalent Chromium Sorption and Desorption in Natural
Soils and Subsoils
R825689C006 Biotransformation of Ordnance Wastes Using Unique Consortia
of Anaerobic Bacteria
R825689C007 The Effect of Environmental Conditions on Reductive Dechlorination
Rates
R825689C008 Lead Sorption, Transport, and Remediation in Natural Soils
and Subsoils
R825689C009 Degradation of Chlorinated Aliphatic Compounds by Nitrifying
Bacteria
R825689C010 Remediation of Contaminated Soil from the Baldock Station
Maintenance Facility
R825689C011 The Effect of Apparent EH, Compound Structure, and Electron
Donor on Anaerobic Biotransformation of Trinitrotoluene and its Metabolites
R825689C012 The "Bubble Wall": A Passive In Situ System
for Treatment and/or Containment of Contaminated Groundwater
R825689C013 Inhibition, Inactivation and Recovery: A Universal Model
for Aerobic Cometabolic Degradation of Aliphatic Compounds
R825689C014 Development, Characterization, and Performance Evaluation
of Ferrous-Ferric Oxide Adsorbents for Metal Removal from Contaminated Groundwater
R825689C015 Redox Transformations of Organic and Inorganic Contaminants
in the Subsurface Environment
R825689C016 Demonstration of a Permeable Barrier Technology for the
Bioremediation of Ground Water Contaminated with Waste Mixtures
R825689C017 Development of a Vitamin B12-Amended Bioremediation Process
for the Reductive Dechlorination of Chlorobiphenyls at all Chlorine Positions
R825689C018 An Investigation of the Chlorinated Hydrocarbon Substrate
Range of the Filamentous Fungus, Graphium sp.
R825689C019 Aerobic Cometabolism of Chloroform, 1,1,1-trichloroethane,
1,1-dichloroethylene, and Other Chlorinated Aliphatic Hydrocarbons by Microbes
Grown on Butane and Propane
R825689C020 Cytochrome P-450: An Emerging Catalyst for the Cometabolism
of Chlorinated Aliphatic Hydrocarbons and Methyl tert-butyl Ether?
R825689C021 In-Situ Measurement of TCE Degradation Using a Single-Well
"Push-Pull" Test
R825689C022 Development and Characterization of Redox Sensors for
Environmental Monitoring
R825689C023 Assessing Metal Speciation in the Subsurface Environment
R825689C024 Simultaneous Removal of the Adsorbable and Electroactive
Metals from Contaminated Soils and Groundwater
R825689C025 Multisolute Sorption and Transport Model for Copper, Chromium,
and Arsenic Sorption on an Iron-Coated Sand, Synthetic Groundwater System
R825689C026 Development of Alkoxysilanes as Slow Release Substrates
for the Anaerobic/Aerobic Transformation of Chlorinated Solvents
R825689C027 Aerobic Cometabolism of Chlorinated Aliphatic Hydrocarbons
by Toluene-Oxidizing Bacteria
R825689C028 Development and Characterization of Sensors and Field
Instrumentation for Monitoring of Environmental Redox Conditions
R825689C029 Aerobic Cometabolism of Methyl tert-butyl Ether by Microorganisms
Grown on Aliphatic Hydrocarbons
R825689C030 Biotransformation of Lead and Chromate by Bacteria
R825689C031 Magnetic Resonance Studies of Heavy Metals in Clays, Zeolites
and Ceramics
R825689C032 Probing the Redox Properties of Environmental Systems: Natural Phenolic Materials
R825689C033 Reductive Dehalogenation at Carbon and Derivatized Carbon
Electrodes
R825689C034 Detection of Microorganisms Capable of Anaerobic Degradation
of Hazardous Substances in Natural Environments
R825689C035 Treatment of Complex Mixtures
R825689C036 Oxidation of Chlorinated Solvents by Methanotrophs
R825689C037 Detection and Assessment of Subsurface Contamination
R825689C038 Design of Reliable and Cost-Effective Mitigation Schemes
R825689C039 Gaseous Stripping of Nonaqueous Phase Liquids from the
Vadose Zone
R825689C040 Anaerobic Microbial Transformation of Homocyclic and Heterocyclic Polynuclear Aromatic Hydrocarbons
R825689C041 Effects of Sorption on Biodegradation of Halogenated Organics
R825689C042 Trace Metal Removal Processes
R825689C043 FASTCHEM Applications and Sensitivity Analysis
R825689C044 Long-term Chemical Transformation of 1,1,1-Trichloroethane
(TCA) and Freon 113 under Aquifer Conditions
R825689C045 In-Situ Anaerobic Biological Treatment of Aromatics in
Groundwater
R825689C046 Use of Starvation and Stress Promoters for Biodegradation
of Hazardous Wastes
R825689C047 Determining and Modeling Diffusion-Limited Sorption and
Desorption Rates of Organic Contaminants in Heterogeneous Soils
R825689C048 Dispersion Modeling of Volatile Organic Emissions from
Ground-Level Treatment Systems
R825689C049 Subsurface Mixing of Nutrients and Groundwater for in-Situ
Bioremediation
R825689C050 Test-Bed Evaluation of In-Situ Bioremediation of Chlorinated
Aliphatic Compounds by Toluene Oxygenase Microorganisms
R825689C051 Demonstration of in-Situ Bioremediation of Chlorinated
Aliphatics by Methanotrophs at St. Joseph
R825689C052 Aquifer Remediation Design in the Presence of Kinetic
Limitations
R825689C053 Determination of Macroscopic Transport Parameters for
Biologically Reacting Solutes in Aquifers
R825689C054 Transformation of Chlorinated Hydrocarbons by Reduced
Metallocoenzymes--Kinetic Model Development and Applications to Environmental
Systems
R825689C055 Microbial Degradation of Toluene Under Sulfate-Reducing
Conditions--The Role of Iron
R825689C056 Transformation of TCE by Methanotrophic Biofilms
R825689C057 Heavy Metals in Ceramic Matrix: Heavy Metals/Clay Interactions
in Ceramic Processing
R825689C058 Radon-222 Method for Locating and Quantifying Contamination
by Residual Non-Aqueous Phase Liquids in the Subsurface
R825689C059 Process Submodel Formulation and Parameter Estimation
for Simulation of Bioremediation
R825689C060 Enhancement of Biodegradation through the Use of Substituted
Porphyrins to Treat Groundwater Contaminated with Halogenated Aliphatics
R825689C061 Field Test of In-Situ Vapor Stripping for Removal of VOCS
from Groundwater
R825689C062 System Design for Enhanced In-Situ Biotransformation of
Carbon Tetrachloride: Application to DOE's Arid Site Integrated Demonstration
R825689C063 Modeling Strategies for Optimizing In-Situ Bioremediation
R825689C064 Anaerobic Treatment of Chlorinated Solvent Contaminated
Groundwater
R825689C065 In Situ Treatment of Chlorinated Solvents
R825689C066 Moffett Field In-Situ Bioremediation Study in Support
of Full Scale Application
R825689C067 Full-Scale Evaluation of In Situ Bioremediation of Chlorinated
Solvent Groundwater Contamination
R825689C068 Upscaling Pore-Scale Hydrodynamics and the Transport of
Reactive Solutes
R825689C069 Pathways of Anaerobic Toluene Metabolism by a Sulfate-Reducing
Bacterium, Strain PRTOL1
R825689C070 Anaerobic Ethylbenzene Oxidation in Denitrifying Strain
EB1
R825689C071 Molecular Approaches to Optimize Starvation Promoter Dricen
TCE Bioremediation in Pseudomonas
R825689C072 Modeling VOC Emissions from Hazardous Waste Sites
R825689C073 Reductive Transformation of Chlorinated Hydrocarbons by
Reduced Ethenes Catalyzed by Vitamin B12 - Mechanistic and Kinetic Studies
R825689C074 Evaluation of Strategies for Full Scale Bioremediation
of the Seal Beach Site Using Anaerobic Microbial Processes
R825689C075 Trace Element Adsorption in Porous Particle Packed Beds
R825689C076 Hydrologic and Biological Factors Affecting Aquifer Clogging
During In-Situ Bioremediation
R825689C077 Full-Scale Evaluation of an Apparatus for Down-well Oxygen
Transfer to Implement In situ Bioremediation at Edwards AFB
R825689C078 Field Testing of Palladium-Catalyzed Hydrodehalogenation
for Chlorinated Hydrocarbon Removal from Groundwater
R825689C079 Physics of Dissolution of Nonaqueous Phase Liquids: Pore
Networks and Field Simulations
R825689C080 Three-Phase Flow in Fractured Media
R825689C081 Effects of Redox Zones on the Fate and Transport of Contaminants
in the Saturated Subsurface; Characterization and Simulation
R825689C082 Biochemical Mechanisms of PCE Dehalogenation by Strain
MS-1, and its Potential for In-situ Bioaugmentation
R825689C083 A Large Scale Model for Anaerobic Bioremediation at the
Seal Beach Site
R825689C084 Mechanisms, Chemistry, and Kinetics of Anaerobic Degradation
of cDCE and Vinyl Chloride
R825689C085 Bioenhanced In-Well Vapor Stripping to Treat Trichloroethylene
(TCE)
R825689C086 Effect of Chemical Structure on the Biodegradability of
Halogenated Hydro-carbons
R825689C087 Trace Element Adsorption in Porous Particle Packed Beds
R825689C088 Arsenic Removal in High Capacity Porous Alumina Packed-Bed
Reactors
R825689C089 Measurement of Interfacial Areas and Mass Transfer Coefficients
Between Residual PCE and Water During Surfactant Enhanced Aquifer Remediation
R825689C090 Proof of Gene Expression During Bioaugmentation
R825689C091 Experimental and Mathematical Study of Biomass Growth
in Pore Networks and its Consequences in Bioremediation
R825689C092 Gene probes for detecting anaerobic alkylbenzene-degrading
bacteria
R825689C093 Investigation of Palladium Catalyzed Hydrodehalogenation
for the Removal of Chlorinated Groundwater Contaminants: Surface Chemistry of
Catalyst Deactivation and Regeneration
R825689C094 Aerobic Methanotrophic Transformation of Biphenyl, Monochlorobiphenyls,
and Dichlorobiphenyls