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Accepted for Publication:
Title: The effect of acidic pH on the ability of Clostridium sporogenes MD1 to take up and retain intracellular potassium
Authors: Michael D. Flythe and James B. Russell
Abstract: At pH values > 5.5, Clostridium sporogenes MD1 accumulated potassium even though it had little protonmotive force, and an ATPase inhibitor (N, N’- dicyclohexylcarbodiimide) prevented this uptake. These results suggested that potassium transport was ATP-driven, and a protonophore (3, 3’, 4’, 5 – tetrachlorosalicylanilide) did not eliminate uptake. However, potassium uptake could also be driven by an artificial pH gradient, and in this case the protonophore acted as an inhibitor. These latter results indicated that the cells also had a protonmotive force driven transporter. When the pH < 5.1, the cells could not retain potassium, rapid efflux was observed, and intracellular volume collapsed.
Additional material pending 7/10/08 GS.
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Last Modified: 07/10/2008
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