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Virucides Test Results

Table 1 - Test Results

Dilution of Virus Virus - Disinfectant* Virus - Control* Cytotoxic-Control
10-1 TTTT ++++ TTTT
10-2 TTTT ++++ TTTT
10-3 T000 ++++ T000
10-4 0000 ++++ 0000
10-5 0000 ++++ 0000
10-6 0000 +++0 0000
10-7 0000 +000 0000
10-8 0000 0000 0000

*Recovery of virus from surfaces demonstrated by cytopathogenic effect, fluorescent antibody, plaque count, animal’ response, or other recognized acceptable technique.

Note: T = toxic; + = virus recovered; 0 = no virus recovered.

Table II-Calculation of the Tissue Culture Infective Dose 50 (TCID50)

Values       Accumulated Values
Virus Dilution Inoculated No. Infected / No. Inoculated No. Infected No. Not Infected No. Infected No. Not Infected No. Infected / No. Inoculated Percent Infected
10-1 4/4 4 0 24 0 24/24 100
10-2 4/4 4 0 20 0 20/20 100
10-3 4/4 4 0 16 0 16/16 100
10-4 4/4 4 0 12 0 12/12 100
10-5 4/4 4 0 8 0 8/8 100
10-6 3/4 3 1 4 1 4/5 80
10-7 1/4 1 3 1 4 1/5 20
10-8 0/4 0 4 0 8 0/8 0

TCID50 = 106.5

Table III-Calculation of the Tissue Culture Lethal Dose 50 (TCLD50)

Values       Accumulated Values
Virus Dilution Inoculated No. Toxic / No. Inoculated No. Toxic No. Not Toxic No. Toxic No. Not Toxic No. Toxic / No. Inoculated Percent Toxic
10-1 4/4 4 0 9 0 9/9 100
10-2 4/4 4 0 5 0 5/5 100
10-3 1/4 1 3 1 3 1/4 25
10-4 0/4 0 4 0 7 0/7 0
10-5 0/4 0 4 0 11 0/11 0
10-6 0/4 0 4 0 15 0/15 0
10-7 0/4 0 4 0 19 0/19 0
10-8 0/4 0 4 0 23 0/23 0

TCID50 = 102.7

Therefore: Virus inactivation = TCID50 - TCLD50 = 103.8 log 10

Claims for virucidal activity for a product must be restricted to those viruses which have actually been tested.

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