Project Number: 5302-22000-008-24
Project Type: Reimbursable

Start Date: Oct 01, 2007
End Date: Sep 30, 2008

Objective:
1) Molecular subtraction and hybridization strategies will be used to develop SSR-enriched genomic libraries. 2) Cloned fragments will be screened to identify SSR containing sequences that will be used to design a new set of PCR-based SSR primers will be designed and validated. 3) New primers will be used for genotyping phylloxera biotypes and assessing genetic diversity of phylloxera populations.

Approach:
1. Develop an innovative molecular approach to identify simple sequence repeat (SSR) loci in phylloxera. 2. Design and validate new SSR primers for genotyping and assessing genetic diversity of Phylloxera. Formerly 5302-22000-008-24R (6/07).