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Research Project:
DEVELOPMENT AND TESTING OF TECHNIQUES TO DETECT TOXOPLASMA GONDII IN WATER
Location: Animal Parasitic Diseases
Project Number: 1265-32000-076-15
Project Type:
Reimbursable
Start Date: May 01, 2005
End Date: Apr 30, 2009
Objective:
To develop test for the detection of Toxoplasma gondii in environmental water supplies.
Approach:
Toxoplasma gondii oocysts will be obtained from feces of experimentally infected cats, purified by sucrose-gradients. Oocysts will be sporulated in 2% sulfuric acid as preservative and then stored at 4 C until used. Known numbers of oocysts will be mixed with water samples and then sensitivity of various methods (bioassay, PCR, monoclonal antibodies) will be tested.
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Last Modified: 11/05/2008
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