Projects

DEVELOPMENT OF NEW TECHNOLOGIES AND METHODS TO ENHANCE THE UTILIZATION AND LONG-TERM STORAGE OF POULTRY, SWINE AND FISH GERMPLASM Appropriated (D)   Accession Number: 411596

Publications

Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial probe 5,5',6,6'tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) using flow cytometry. - (Book/Chapter)

Guthrie, H.D., Welch, G.R. Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial probe 5,5',6,6'tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) using flow cytometry.. Book Chapter. Methods in Molecular Biology, vol. 477: Advanced Protocols for Oxidative Stress I, Ed. D. Armstrong, DOI: 10.1007/978-1-60327-517-2_1, Humana Press New York, NY 07512. 2008. pp. 89-97

Effects of osmolality on testicular striped bass (Morone saxatilis) sperm energy status - (Peer Reviewed Journal)

Guthrie, H.D., Woods, L.C., III, Long, J.A., Welch, G.R. 2008. Effects of osmolality on inner mitochondrial transmembrane potential and ATP content in spermatozoa recovered from the testes of striped bass (Morone saxatilis). Theriogenology. 69:1007-12.

Mitochondrial function and reactive oxygen species action in relation to boar motility. - (Peer Reviewed Journal) - (30-Oct-07)

Use of fluorescence-activated flow cytometry to determine membrane lipid peroxidation during hypothermic liquid storage and freeze-thawing of viable boar sperm loaded with C11-BODIPY 581/591 - (Peer Reviewed Journal)

Guthrie, H.D., Welch, G.R. 2007. Use of fluorescence-activated flow cytometry to determine membrane lipid peroxidation during hypothermic liquid storage and freeze-thawing of viable boar sperm loaded with C11-BODIPY 581/591. Journal of Animal Science. 85:1402-1411.

Lactation Induction As a Predictor of Post-Parturition Transgene Expression in Bovine Milk - (Peer Reviewed Journal)

Powell, A.M., Kerr, D.E., Guthrie, H.D., Wall, R.J. 2007. Lactation induction as a predictor of post-parturition transgene expression in bovine milk. Journal of Dairy Research. 74(2):247-254.

Determination of Intracellular Reactive Oxygen Species and High Mitochrondrial Membrane Potential in Viable Boar Sperm Using Fluorescence Activated Flow Cytometry - (Peer Reviewed Journal)

Guthrie, H.D., Welch, G.R. 2006. Determination of intracellular reactive oxygen species and high mitochrondrial membrane potential in viable boar sperm using fluorescence activated flow cytometry. Journal of Animal Science. 84:2089-2100.

Treatment of Boar Sperm with Respiration Inhibitor Menadione: Effects on Motility, Reactive Oxygen Species (Ros), Mitochondrial Transmembrane Potential (Mmp), and Atp Content - (Peer Reviewed Journal)

Guthrie, H.D., Long, J.A., Welch, G.R., Grimm, N.E. 2006. Treatment of boar sperm with respiration inhibitor menadione: effects on motility, reactive xygen species (ros), mitochondrial transmembrane potential (mmp), and atp content [abstract]. Reproduction, Fertility and Development. 18:259-260.

Validation of a Rapid, Large-Scale Assay to Quantify Atp Levels in Spermatozoa - (Peer Reviewed Journal)

Long, J.A., Guthrie, H.D. 2006. Validation of a rapid, large-scale assay to quantify atp levels in spermatozoa. Theriogenology. 65:1620-1630.

New Assays for Reactive Oxygen Species (Ros) Formation and Mitochondrial Membrane Potential in Fresh and Cryopreserved Boar Spermatozoa - (Abstract)

Guthrie, H.D., Welch, G.R. 2005. New assays for reactive oxygen species (ros) formation and mitochondrial membrane potential in fresh and cryopreserved boar spermatozoa [abstract]. 7th International Conference on Pig Reproduction National Conference. p. 141.

Follicular Expression of a Human Beta-Cell Leukemia/lymphoma-2 (Bcl-2)transgene Does Not Decrease Atresia Or Increase Ovulation Rate in Swine - (Peer Reviewed Journal)

Guthrie, H.D., Wall, R.J., Pursel, V.G., Foster Frey, J.A., Donovan, D.M., Dawson, H.D., Welch, G.R., Garrett, W.M. 2005. Follicular expression of a human B-cell leukemia/lymphoma-2 (Bcl-2)transgene does not decrease atresia or increase ovulation rate in swine. Journal Of Reproduction, Fertility And Development. 17(4):457-468.

Effects of Hypothermic Liquid Storage and Cryopreservation on Basal and Induced Plasma Membrane Phospholipid Disorder and Acrosome Exocytosis in Boar Spermatozoa - (Peer Reviewed Journal)

Guthrie, H.D., Welch, G.R. 2005. Effects of hypothermic liquid storage and cryopreservation on basal and induced plasma membrane phospholipid disorder and acrosome exocytosis in boar spermatozoa. Reproduction, Fertility and Development. 17:467-477.

Impact of Extender and Cryopreservation on Membrane and Morphological Integrity in Boar Sperm - (Proceedings/Symposium)

Guthrie, H.D., Welch, G.R. 2004. Impact of extender and cryopreservation on membrane and morphological integrity in boar sperm. Theriogenology. 63:396-410.

Follicular Expression of a Human Beta-Cell Leukemia/lymphoma-2 (Bcl-2)transgene Does Not Decrease Atresia Or Increase Ovulation Rate in Swine - (Abstract)

Guthrie, H.D., Wall, R.J., Pursel, V.G., Donovan, D.M., Welch, G.R., Garrett, W.M. 2004. Follicular expression of a human beta-cell leukemia/lymphoma-2 (bcl-2)transgene does not decrease atresia or increase ovulation rate in swine [abstract]. Society for the Study of Reproduction Annual Meeting. p. 275.

The Follicular Phase in Pigs: Follicle Populations, Circulating Hormones, Follicle Factors and Oocytes - (Peer Reviewed Journal)

Guthrie, H.D. 2005. The follicular phase in pigs: follicle populations, circulating hormones, follicle factors and oocytes. Journal of Animal Science. 83(E Suppl.):E79-E89.

Follicular Expression of a Human Beta-Cell Leukemia/lymphoma-2 (Bcl-2)transgene Does Not Decrease Atresia Or Increase Ovulation Rate in Swine. - (Abstract)

Guthrie, H.D., Wall, R.J., Pursel, V.G., Foster Frey, J.A., Donovan, D.M., Welch, G.R., Garrett, W.M. 2004. Follicular expression of a human beta-cell leukemia/lymphoma-2 (bcl-2)transgene does not decrease atresia or increase ovulation rate in swine. Society for the Study of Reproduction Annual Meeting. Abstract. p275.

Cag Repeat Lengths in X and Y-Bearing Sperm Indicate That Gender Bias During Transmission of Huntington's Disease Gene Is Determined in the Embryo. - (Peer Reviewed Journal)

Kovtun, I.V., Welch, G.R., Guthrie, H.D., Hafner, K.L., Mcmurray, C.T. 2004. Cag repeat lengths in x- and y-bearing sperm indicate that gender bias during transmission of huntington's disease gene is determined in the embryo. Journal of Biological Chemistry. Vol. 279, No. 10, Issue of March 5, pp. 9389-9391.

The Follicular Phase in Pigs: Follicle Populations, Circulating Hormones, Follicle Factors and Oocytes - (Abstract)

Guthrie, H.D. 2005. The follicular phase in pigs: follicle populations, circulating hormones, follicle factors and oocytes [abstract]. 37th Midwestern Sectional Meeting of the American Society of Animal Science and The American Society of Dairy Science Association. Paper No. 228.

Offspring Sex Ratio in Deer Male Reproductive Physiology and Offspring Sex Ratio in Odocoileus Virginianus. - (Peer Reviewed Journal) - (12-Sep-03)

Increasing the Holding Time at 15 C of Boar Spermatozoa Before Freezing Decreases Fertility after Thawing. - (Peer Reviewed Journal)

Guthrie, H.D., Welch, G.R. 2003. Increasing the holding time at 15 c of boar spermatozoa before freezing decreases fertility after thawing. [abstract]. Journal of Animal Science. 81(2):88.

In Vitro and in Vivo Developmental Competence of Ovulated and in Vitro Matured Porcine Oocytes Activated by Electrical Activation - (Peer Reviewed Journal)

Zhu, J., King, T., Dobrinsky, J.R., Harkness, L., Ferrier, T., Bosma, W., Schreier, L.L., Guthrie, H.D., Desousa, P., Wilmut, I. 2003. In vitro and in vivo developmental competence of ovulated and in vitro matured porcine oocytes activated by electrical activation. Cloning and Stem Cells. Volume 5(4):355-65.

Overnight Holding Time Before Cryopreservation Reduces Fertility of Frozen-Thawed Spermatozoa - (Popular Publication) - (02-Jan-03)