ARS | Publication request: Functional Proteomic Plasmid-Based Integrated Workcell for High-Throughput Transformation of Bl21 De3 E. Coli for Expression in Vivo with Piromyces Strain Xylose Isomerase

Submitted to: Meeting Abstract
Publication Type: Abstract
Publication Acceptance Date: April 25, 2006
Publication Date: April 25, 2006
Citation: Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Liu, S., Qureshi, N., Cotta, M.A., Skory, C.D., Gorsich, S.W., Farrelly, P.J. 2006. Functional proteomic plasmid-based integrated workcell for high-throughput transformation of BL21 DE3 E. coli for expression in vivo with piromyces strain xylose isomerase [abstract]. Midwest Laboratory Robotics Information Group. p. 2.

Technical Abstract: To date a fully automated process for the mass transformation of cDNA libraries into yeast or bacteria does not exist. In this work we present the first in a series of experiments demonstrating it is possible to transform yeast or bacteria in high throughput starting with colonies of the desired target cells. The reagents used are kept at room temperature. The resulting transformed cells are processed for in vivo expressed protein and the protein analyzed using plate assays using protocols developed for a fully automated workcell. Here we provide data generated from the protocols used to validate these processes.