ARS | Publication request: Mass Transformation of Plasmid Libraries of Cdna Or Mutagenized Clone Sets into Yeast Or Bacteria Using a Functional Proteomic Robotic Workcell

Submitted to: Meeting Abstract
Publication Type: Abstract
Publication Acceptance Date: January 13, 2006
Publication Date: January 13, 2006
Citation: Hughes, S.R., Riedmuller, S., Li, X., Qureshi, N., Liu, S., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2006. Mass transformation of plasmid libraries of cDNA or mutagenized clone sets into yeast or bacteria using a functional proteomic robotic workcell [abstract]. PepTalk 2006. p. 10.

Technical Abstract: To date a fully automated process for the mass transformation of cDNA libraries into yeast or bacteria does not exist. In this work, we present the first in a series of experiments demonstrating it is possible to transform yeast or bacteria in high throughput starting with colonies of the desired target cells. The reagents used are kept at room temperature. The resulting transformed cells are processed for in vivo expressed protein and the protein analyzed using plate assays using protocols developed for a fully automated workcell. Here we provide data generated from the protocols used to validate these processes.