Projects

OPPORTUNITIES & LIMITS TO PERTURBING FORAGE PLANT BIOCHEMISTRY, GROWTH, & DEVELOPMENT FOR IMPROVING FORAGE NUTRITIONAL BENEFITS IN DAIRY SYS Appropriated (D)   Accession Number: 412700

REDESIGNING FORAGE GERMPLASM AND PRODUCTION SYSTEMS FOR EFFICIENCY, PROFIT, AND SUSTAINABILITY OF DAIRY FARMS Appropriated (D)   Accession Number: 412703

STREAMLINED METHOD FOR BIOMASS WHOLE-CELL-WALL STRUCTURAL PROFILING Reimbursable (R)   Accession Number: 411096

POLYPHENOL OXIDASE-GENERATED QUINONES: BIOCHEMICAL MECHANISM AND CONSEQUENCES OF THEIR INTERACTIONS WITH PROTEINS Reimbursable (R)   Accession Number: 409948

WHAT IS THE EXTENT OF METABOLIC PLASTICITY IN THE LIGNIFICATION PROCESS, AND CAN IT BE EXPLOITED? Reimbursable (R)   Accession Number: 407214

Publications

COMPARING CORN TYPES FOR DIFFERENCES IN CELL WALL CHARACTERISTICS AND p-COUMAROYLATION OF LIGNIN - (Peer Reviewed Journal) - (14-Aug-08)

Coniferyl Ferulate Incorporation into Lignin Enhances the Alkaline Delignification and Enzymatic Degradation of Cell Walls - (Peer Reviewed Journal)

Grabber, J.H., Hatfield, R.D., Ralph, J., Lu, F. 2008. Coniferyl Ferulate Incorporation into Lignin Enhances the Alkaline Delignification and Enzymatic Degradation of Cell Walls. Biomacromolecules.

VARIABILITY OF GRASS LIGNIN ACYLATION - (Abstract)

Hatfield, R.D., Marita, J.M., Frost, K. 2008. Variability of grass lignin acylation [abstract]. The Pan American Congress on Plants and Bioenergy, June 22-25, 2008, Merida, Mexico. Abstract M705, p. 36.

A NOVEL RED CLOVER HYDROXYCINNAMOYL TRANSFERASE HAS ENZYMATIC ACTIVITIES CONSISTENT WITH A ROLE IN PHASALIC ACID [2-O-(CAFFEOYL)-L-MALATE] BIOSYNTHESIS - (Abstract)

Sullivan, M.L., Hatfield, R.D. 2008. A novel red clover hydroxycinnamoyl transferase has enzymatic activities consistent with a role in phasalic acid [2-o-(caffeoyl)-l-malate] biosynthesis [abstract]. Gordon Research Conference on Plant Molecular Biology, July 13-18, 2008, Holderness, New Hampshire.

CHARACTERISTICS OF POLYPHENOL OXIDASES - (Abstract)

Hatfield, R.D., Sullivan, M.L., Schmitz, G. 2008. Characteristics of polyphenol oxidases [abstract]. The 2008 Joint Meeting of the American Society of Plant Biologists and the Sociedad Mexicana de Bioquimica Rama: Bioquimica y Biologia Molecular de Plantas, June 26-July 1, 2008, Merida, Mexico. Abstract P04010. p. 103.

CHARACTERIZATION OF A p-COUMAROYL TRANSFERASE RESPONSIBLE FOR THE INCORPORATION OF pCA INTO GRASS CELL WALLS - (Abstract)

Hatfield, R.D., Marita, J.M. 2008. Characterization of a p-coumaroyl transferase responsible for the incorporation of pCA into grass cell walls [abstract]. Ferulate '08: An International Conference on Hydroxycinnamates and Related Plant Phenolics, August 25-27, 2008, Minneapolis, Minnesota. p. 53.

COPOLYMERIZATION OF CONIFERYL FERULATE WITH MONOLIGNOLS: IMPACT ON LIGNIN FORMATION AND ALKALINE DELIGNIFICATION OF MAIZE CELL WALLS - (Abstract)

Grabber, J. H., Ralph, J., Hatfield, R. D., Lu, F. 2008. Copolymerization of coniferyl ferulate with monolignols: impact on lignin formation and alkaline delignification of maize cell walls. Proceedings of Ferulate '08. p. 36.

Coniferyl ferulate incorporation into lignin dramatically enhances the delignification and enzymatic hydrolysis of maize cell walls - (Abstract)

Grabber, J. H., Hatfield, R. D., Ralph, J., Lu, F. 2008. Coniferyl ferulate incorporation into lignin dramatically enhances the delignification and enzymatic hydrolysis of maize cell walls [abstract]. Proceedings of Biotechnology for Fuels and Chemicals Symposium. 8(05):56.

ALFALFA: FORAGE CROP OF THE FUTURE - (Proceedings/Symposium)

Martin, N.P., Mertens, D.R., Hatfield, R.D., Jung, H.J. 2008. Alfalfa: forage crop of the future. In: Proceedings of the 28th Kentucky Alfalfa Conference, February 21, 2008, Cave City, Kentucky, p. 17-26.

CLONING OF AN ALFALFA POLYPHENOL OXIDASE GENE AND EVALUATION OF ITS POTENTIAL IN PREVENTING POSTHARVEST PROTEIN DEGRADATION - (Peer Reviewed Journal)

Sullivan, M.L., Hatfield, R.D., Samac, D.A. 2008. Cloning of an alfalfa polyphenol oxidase gene and evaluation of its potential in preventing postharvest protein degradation. Journal of the Science of Food and Agriculture. 88(8):1406-1414.

DESIGNING LIGNINS FOR IMPROVED BIOMASS PROCESSING - (Proceedings/Symposium)

Ralph, J., Hatfield, R.D., Grabber, J.H., Lu, F., Kim, H., Marita, J.M. 2008. Designing lignins for improved biomass processing. In: Proceedings of the Funcfiber 2008 International Symposium on the Biology and Biotechnology of Wood, March 10-12, 2008, Umea, Sweden. p. 14

THREE POLYPHENOL OXIDASES FROM RED CLOVER (TRIFOLIUM PRATENSE) DIFFER IN ENZYMATIC ACTIVITIES AND ACTIVATION PROPERTIES - (Peer Reviewed Journal)

Schmitz, G.E., Sullivan, M.L., Hatfield, R.D. 2008. Three polyphenol oxidases from red clover (Trifolium pratense) differ in enzymatic activities and activation properties. Journal of Agricultural and Food Chemistry. 56(1):272-280.

CHARACTERISTICS OF POLYPHENOL OXIDASES FROM RED CLOVER (TRIFOLIUM PRATENSE) - (Abstract)

Schmitz, G.E., Hatfield, R.D., Sullivan, M.L. 2007. Characteristics of polyphenol oxidases from red clover (Trifolium pratense)[abstract]. In: Program and Abstract Book of the Plant Biology and Botany 2007 Joint Congress, July 8-11, 2007, Chicago, Illinois. p. 23.

LIGNIN ACYLATION IN GRASSES - (Abstract)

Marita, J.M., Hatfield, R.D., Frost, K.E. 2007. Lignin acylation in grasses [abstract]. In: Program and Abstract Book of the Plant Biology and Botany 2007 Joint Congress, July 8-11, 2007, Chicago, Illinois. p. 106.

Red clover polyphenol oxidase reduces ruminal lipolysis in in vitro batch culture - (Proceedings/Symposium)

Lee, M.R., Minchin, F.R., Hatfield, R.D., Sullivan, M.L. 2007. Red clover polyphenol oxidase reduces ruminal lipolysis in in vitro batch culture. In: Proceedings of the British Society of Animal Science. April 2-4, 2007, Southport, United Kingdom. p. 25.

ON-FARM PRETREATMENT TECHNOLOGIES FOR IMPROVING ENZYMATIC DIGESTIBILITY OF CELLULOSE AND HEMICELLULOSE PRESENT IN PERENNIAL GRASS - (Proceedings/Symposium)

Digman, M.F., Shinners, K.J., Dien, B.S., Hatfield, R.D., Li, X., Muck, R.E., Weimer, P.J. 2007. On-farm pretreatment technologies for improving enzymatic digestibility of cellulose and hemicellulose present in perennial grass. In: Proceedings of 2007 Annual Meeting of American Society of Agricultural and Biological Engineers, June 17, 2007, Minneapolis, Minnesota. Paper No. 071021.

NUTRITIONAL CHEMISTRY OF FORAGES - (Book/Chapter)

Hatfield, R.D., Jung, H.G., Broderick, G.A., Jenkins, T. 2007. Nutritional chemistry of forages. In: Barnes, R.F., Nelson, C.J., Moore, K.J., Collins, M., editors. The Science of Grassland Agriculture. Sixth Edition of Forages, Volume II. Ames, IA:Blackwell Publishing. p. 467-485.

CELL-WALL COMPOSITION OF SMOOTH BROMEGRASS PLANTS SELECTED FOR DIVERGENT FIBER CONCENTRATION - (Peer Reviewed Journal)

Casler, M.D., Hatfield, R.D. 2006. Cell-wall composition of smooth bromegrass plants selected for divergent fiber concentration. Journal of Agriculture and Food Chemistry. 54:8206-8211.

Transforming Forage Plants to Increase Nitrogen Utilization in Dairy Systems: What Are the Possibilities? - (Abstract)

Hatfield, R.D., Grabber, J.H., Sullivan, M.L., Waghorn, G., Mccaslin, M. 2006. Transforming Forage Plants to Increase Nitrogen Utilization in Dairy Systems: What Are the Possibilities? Journal of Dairy Science. 89(supplement 1):633.

MICROGRAVITY EFFECTS ON LEAF MORPHOLOGY, CELL STRUCTURE, CARBON METABOLISM AND MRNA EXPRESSION OF DWARF WHEAT - (Peer Reviewed Journal)

Stutte, G.W., Monje, O., Hatfield, R.D., Paul, A.-L., Ferl, R.J., Simone, C.G. 2006. Microgravity effects on leaf morphology, cell structure, carbon metabolism and mRNA expression of dwarf wheat. Planta. 224:1038-1049.

REINVENTING ALFALFA FOR DAIRY CATTLE AND NOVEL USES - (Proceedings/Symposium)

Martin, N.P., Hatfield, R.D., Mertens, D.R. 2005. Reinventing alfalfa for dairy cattle and novel uses. Proceedings of the 35th California Alfalfa & Forage Symposium. p. 299-311.

POLYPHENOL OXIDASE AND O-DIPHENOLS INHIBIT POST-HARVEST PROTEOLYSIS IN RED CLOVER AND ALFALFA - (Peer Reviewed Journal)

Sullivan, M.L., Hatfield, R.D. 2006. Polyphenol oxidase and o-diphenols inhibit post-harvest proteolysis in red clover and alfalfa. Crop Science. 46:662-670.

LIGNIN ACYLATION IN GRASSES - (Abstract)

Hatfield, R.H., Marita, J.M., Frost, K. 2005 Lignin acylation in grasses [abstract]. Cell Wall Biosynthesis Meeting, Paper No.78

POLYPHENOL OXIDASE ACTIVITY, O-DIPHENOLS, BROWNING, AND IN VITRO PROTEOLYTIC INHIBITION IN GRASSES - (Abstract)

Hatfield, R.D., Marita, J.M., Brink, G.E. 2005. Polyphenol oxidase activity, o-diphenols, browning, and in vitro proteolytic inhibition in grasses [abstract]. ASA-CSSA-SSSA International Annual Meetings. Paper No. 241-3.

DETERMINATION OF POLYPHENOL OXIDASE ACTIVITY (PPO) AND PROTEOLYTIC INHIBITION IN GRASSES - (Abstract)

Marita, J.M., Hatfield, R.D., Brink, G.E. 2005. Determination of polyphenol oxidase activity (PPO) and proteolytic inhibition in grasses [abstract]. Plant Biology. p. 210.

USING THE RED CLOVER POLYSHENOL OXIDASE GENE TO INHIBIT PROTEOLYTIC ACTIVITY IN ALFALFA - (Proceedings/Symposium)

Hatfield, R.D., Sullivan, M.L. Muck, R.E. 2005. Using the red clover polyphenol oxidase gene to inhibit proteolytic activity in alfalfa. In: Park, R.S., Stronge, M.D., editors. Silage Production and Utilization. XIVth International Silage Conference, a Satellite Workshop of the XXth International Grassland Congress, July 2005, Belfast, Northern Ireland. p. 212.

USDA ARS NUTRIENT MANAGEMENT RESEARCH LABORATOY 50% SUBMISSION - (Abstract)

Marita, J.M., Hatfield, R.D., Brink, G.E. 2005. Polyphenol oxidase activity and in vitro proteolytic inhibition in grasses [abstract]. XIVth International Silage Conference. p. 210.

POTENTIAL BENEFITS OF PLANT MODIFICATION OF ALFALFA AND CORN SILAGE TO DAIRY DIETS - (Review Article)

Hartnell, G.F., Hatfield, R.D., Mertens, D.R., and Martin, N.P. 2005. Potential benefits of plant modification of alfalfa and corn silage to dairy diets. In: Proceedings of the 20th Southwest Nutrition and Management Conference, University of Arizona, Tucson. p. 156-172.

METHYL ESTERIFICATION DIVERGENTLY AFFECTS THE DEGRADABILITY OF PECTIC URONOSYLS IN NONLIGNIFIED AND LIGNIFIED MAIZE WALLS - (Peer Reviewed Journal)

Grabber, J.H. and R.D. Hatfield. 2005. Methyl Esterification Divergently Affects the Degradability of Pectic Uronosyls in Nonlignified and Lignified Maize Walls. Journal of Agriculture and Food Chemistry. 53: 1546-1549

POLYPHENOL OXIDASE ACTIVITY AND IN VITRO PROTEOLYTIC INHIBITION IN GRASSES - (Abstract)

Marita, J.M., Hatfield, R.D., Brink, G.E. 2005. Polyphenol oxidase activity and in vitro proteolytic inhibition in grasses. In: O'Mara, F.P., Wilkins, L., 't Mannetje, D.K., Lovett, R.A., Rogers, M., Boland, T.J., editors. XX International Grassland Congress: Offered Papers. Paper# 220.

CLONING AND CHARACTERIZATION OF RED CLOVER POLYPHENOL OXIDASE CDNAS AND EXPRESSION OF ACTIVE PROTEIN IN ESCHERICHIA COLI AND TRANSGENIC ALFALFA - (Peer Reviewed Journal)

Sullivan, M.L., Hatfield, R.D., Thoma, S., Samac, D.A. 2004. Cloning of red clover polyphenol oxidase cdnas and expression of active protein in escherichia coli and transgenic alfalfa. Plant Physiology. 136:3234-3244.

PEROXIDASE-DEPENDENT CROSS-LINKING REACTIONS OF P-HYDROXYCINNAMATES IN PLANT CELL WALLS - (Peer Reviewed Journal)

Ralph, J., Bunzel, M., Marita, J.M., Hatfield, R.D., Lu, F., Kim, H., Schatz, P.F., Grabber, J.H., Steinhart, H. 2004. Peroxidase-dependent cross-linking reactions of p-hydroxycinnamates in plant cell walls. Phytochemistry Reviews. 3:79-96

LIGNINS AND FERULATE-CONIFERYL ALCOHOL CROSS-COUPLING PRODUCTS IN CEREAL GRAINS - (Peer Reviewed Journal)

Bunzel, M., Ralph, J., Lu, F., Hatfield, R.D., Steinhart, H. 2004. Lignins and ferulate-coniferyl alcohol cross-coupling products in cereal grains. Journal of Agricultural and Food Chemistry. 52:6496-6502.

POLYPHENOL OXIDASE AND O-DIPHENOLS INHIBIT POST-HARVEST PROTEOLYSIS IN RED CLOVER AND ALFALFA - (Abstract)

Sullivan, M.L., Hatfield, R.D., Muck, R.E. Polyphenol oxidase and o-diphenols inhibit post-harvest proteolysis in red clover and alfalfa. 2004. North American Alfalfa Improvement Conference. Available: www.naaic.org/meeting/National/2004NAAIC8TC/2004abstracts/msullivan.pdf.

PROTEIN-BINDING POLYPHENOLS ENHANCE NITROGEN CYCLING AND PROFITABILITY OF DAIRY FARMS - (Proceedings/Symposium)

Grabber, J.H., Broderick, G.A., Sullivan, M.L., Hatfield, R.D., Powell, J.M., Rotz, C.A., Muck, R.E., Ehlke, N.J. 2004. Protein-binding polyphenols enhance nitrogen cycling and profitability of dairy farms. In: 228th ACS National Meeting, August 22-26, 2004, Philadelphia, Pennsylvania. 2004 CDROM.

LIGNINS: NATURAL POLYMERS FROM OXIDATIVE COUPLING OF 4-HYDROXYPHENYLPROPANOIDS - (Peer Reviewed Journal)

Ralph, J., Lundquist, K., Brunow, G., Lu, F., Kim, H., Schatz, P.F., Marita, J.M., Hatfield, R.D., Ralph, S.A., Christensen, J.H., Boerjan, W. 2004. Lignins: natural polymers from oxidative coupling of 4-hydroxyphenylpropanoids. Phytochemistry Reviews 3(1):29-60.

POLYMERIZATION RATE AND PH EFFECTS ON LIGNIN FORMATION AND LIGNIN-MATRIX INTERACTIONS IN MAIZE WALLS - (Proceedings/Symposium) - (12-Jun-03)

APOPLASTIC PH AND MONOLIGNOL ADDITION RATE EFFECTS ON LIGNIN FORMATION AND CELL WALL DEGRADABILITY IN MAIZE - (Peer Reviewed Journal)

Grabber, J.H., Hatfield, R.D., Ralph, J. 2003. Apoplastic ph and monolignol addition rate effects on lignin formation and cell wall degradability in maize. Journal of Agricultural and Food Chemistry. 51:4984-4989.

CLONING OF RED CLOVER AND ALFALFA POLYPHENOL OXIDASE GENES AND EXPRESSION OF ACTIVE ENZYMES IN TRANSGENIC ALFALFA - (Book/Chapter)

Sullivan, M.L., Thoma, S., Samac, D.A., Hatfield, R.D. 2004. Cloning of red clover and alfalfa polyphenol oxidase genes and expression of active enzymes in transgenic alfalfa. In: Hopkins, A., Wang, Z-Y., Mian, R., Sledge, M., Barker, R.W. editors. Molecular Breeding of Forage and Turf. Netherlands: Kluwer Academic Publishers. p. 189-195.

ALFALFA POLYPHENOL OXIDASE IS EXPRESSED IN FLOWERS AND DEVELOPING SEEDS - (Proceedings/Symposium)

SULLIVAN,M.L., EVANS,M., THOMA,S., HATFIELD,R.D., ALFALFA POLYPHENOL OXIDASE IS EXPRESSED IN FLOWERS AND DEVELOPING SEEDS, MEETING ABSTRACT, 2003.

DETERMINING THE ROLE OF P-COUMARYL TRANSFERASE IN LIGNIFICATION OF GRASSES - (Abstract) - (28-Feb-03)

MODIFICATIONS IN LIGNIN OF TRANSGENIC ALFALFA DOWN-REGULATED IN COMT AND CCOAOMT - (Proceedings/Symposium)

MARITA, J.M., RALPH, J., HATFIELD, R.D., GUO, D., CHEN, F., DIXON, R. MODIFICATIONS IN LIGNIN OF TRANSGENIC ALFALFA DOWN-REGULATED IN COMT AND CCOAOMT. INTERNATIONAL SYMPOSIUM ON WOOD AND PULPING CHEMISTRY. 2003.

CLONING OF RED CLOVER AND ALFALFA POLYPHENOL OXIDASE GENES AND EXPRESSION OF ACTIVE ENZYMES IN TRANSGENIC ALFALFA - (Proceedings/Symposium)

Sullivan, M.L., Thoma, S., Samac, D., Hatfield, R.D. Cloning of red clover and alfalfa polyphenol oxidase genes and expression of active enzymes in transgenic alfalfa. Molecular Breeding of Forage and Turf Conference. 2003. p. 189-195

REDESIGNING ALFALFA FOR DAIRY CATTLE - (Proceedings/Symposium)

Martin, N.P., Hatfield, R.D., Mertens, D.R. 2003. Redesigning alfalfa for dairy cattle. In: Land O Lakes Feed Division Leading Producer Conference, January 21, 2003, Wisconsin Dells, Wisconsin. p. 1-3.

NUCLEAR MAGNETIC RESONANCE SPECTRA OF TWO TYPES OF LIGNIN - (Peer Reviewed Journal)

FUKUSHIMA, R.S., HATFIELD, R.D. NUCLEAR MAGNETIC RESONANCE SPECTRA OF TWO TYPES OF LIGNIN. Pesquisa Agropecuaria Brasileira. 2003a. v. 38. p. 505-511.

PHENOLIC COMPOSITION OF DIOXANE LIGNINS AS DETERMINED BY NITROBENZENE OXIDATIVE REACTION - (Peer Reviewed Journal)

FUKUSHIMA, R.S., HATFIELD, R.D. PHENOLIC COMPOSITION OF DIOXANE LIGNINS AS DETERMINED BY NITROBENZENE OXIDATIVE REACTION. MEETING ABSTRACT. 2003b. v. 38. p. 373-378.

VARIATIONS IN THE CELL WALL COMPOSITION OF MAIZE BROWN MIDRIB MUTANTS - (Peer Reviewed Journal)

MARITA, J.M., VERMERRIS, W., RALPH, J., HATFIELD, R.D. VARIATIONS IN THE CELL WALL COMPOSITION OF MAIZE BROWN MIDRIB MUTANTS. Journal of Agriculture and Food Chemistry. 2003. v. 51. p. 1313-1321. v. 51. pp. 1313-1321.

MODIFICATIONS IN LIGNIN OF TRANSGENIC ALFALFA DOWNREGULATED IN CAFFEIC ACID 3-0-METHYLTRANSFERASE AND CAFFEOYL COENZYME A 3-0-METHYLTRANSFERASE - (Abstract) - (12-Dec-02)

SINAPATE DEHYDRODIMERS AND SINAPALE-FERULATE HETERODIMERS IN CEREAL DIETARY FIBER - (Peer Reviewed Journal) - (19-Nov-02)

STRUCTURAL AND COMPOSITIONAL MODIFICATIONS IN LIGNIN OF TRANSGENIC ALFALFA DOWN-REGULATED IN CAFFEIC ACID 3-O-METHYLTRANSFERASE AND CAFFEOYL COA 3-O-METHYLTRANSFERASE - (Peer Reviewed Journal) - (09-Sep-02)

MODEL STUDIES OF FERULATE-CONIFERYL ALCOHOL CROSS PRODUCT FORMATION IN PRIMARY MAIZE WALLS: IMPLICATIONS FOR LIGNIFICATION IN GRASSES - (Peer Reviewed Journal) - (30-Jul-02)

CHANGES IN CARBOHYDRATE COORDINATED PARTITIONING AND CELL WALL REMODELING WITH STRESS-INDUCED PATHOGENESIS IN WHEAT SHEATHS - (Peer Reviewed Journal) - (28-Mar-02)

CHARACTERIZATION OF RED CLOVER POLYPHENOL OXIDASE - (Abstract) - (28-Feb-02)

ISOLATION AND CHARACTERIZATION OF POLYPHENOL OXIDASE GENES FROM ALFALFA - (Abstract) - (28-Feb-02)

CLONING OF RED CLOVER POLYPHENOL OXIDASE CDNAS AND CHARACTERIZATION OF ACTIVE PROTEIN EXPRESSED IN TRANSGENIC ALFALFA - (Abstract) - (28-Feb-02)

NMR PROOF THAT LIGNINS OF COMT-DEFICIENT TRANSGENIC AND MUTANT ANGIOSPERMS INCORPORATE 5-HYDROXCONIFERYL ALCOHOL - (Proceedings/Symposium) - (17-Feb-02)

ELUCIDATION OF NEW STRUCTURES IN LIGNINS OF CAD- AND COMT- DEFICIENT PLANTS BY NMR - (Peer Reviewed Journal) - (16-Nov-00)