Award Abstract #0619163
MRI: Acquisition of an Liquid Chromatograph Mass Spectrometer to Enhance Research and Research Training Opportunities at San Francisco State University

NSF Org:	CHE Division of Chemistry
Initial Amendment Date:	July 25, 2006
Latest Amendment Date:	January 18, 2007
Award Number:	0619163
Award Instrument:	Standard Grant
Program Manager:	Carlos A. Murillo CHE Division of Chemistry MPS Directorate for Mathematical & Physical Sciences
Start Date:	August 1, 2006
Expires:	July 31, 2009 (Estimated)
Awarded Amount to Date:	$440204
Investigator(s):	Ten-Yang Yen ryen@sfsu.edu (Principal Investigator) Scott Gronert (Former Principal Investigator) Bruce Macher (Co-Principal Investigator) Ten-Yang Yen (Former Co-Principal Investigator)
Sponsor:	San Francisco State University 1600 Holloway Ave San Francisco, CA 94132 415/338-7091
NSF Program(s):	MAJOR RESEARCH INSTRUMENTATION
Field Application(s):
Program Reference Code(s):	OTHR,9198,9141,0000
Program Element Code(s):	1189

ABSTRACT

With support from the Major Research Instrumentation (MRI) Acquisition Program, the Department of Chemistry and Biochemistry at San Francisco State University will acquire a highly sensitive, rapid-scanning ion-trap mass spectrometer with a versatile liquid chromatography interface. The instrument will be used for research on a variety of topics including (a) organic reaction mechanisms and molecular recognition; (b) protein structure and the identification of plasma membrane proteins; (c) inhibitor interactions with the prostate-specific membrane antigen; (d) protein-protein interactions related to the styrene metabolism pathway; (e) covalent inhibitors of orotidine-5'-monophosphate decarboxylase; (f) mapping palmitoylation sites on Wnt proteins related to embryogenesis; (g) characterization of cell surface kinasis and identification of

binding partners, and (f) protein expression in rice related to the development of

bacterial pathogen resistance.

Liquid Chromatography with mass spectrometry detection (LCMS) is a powerful technique used for the separation and analysis of complex mixtures. It probes structural details and molecular weights of organic, bioorganic and inorganic molecules. It has become an indispensable tool for solving problems in virtually all fields requiring chemical analysis. Undergraduates and graduate students will use the instrument in their research projects and educational training activities. Mass spectrometry has been integrated into the chemistry/biochemistry curriculum at several levels and is a component of several courses.

PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH

(Showing: 1 - 5 of 5).

Lee, T.; Singh, R.;Chavez, A.; Yen, T.Y. and Macher, B..  "MS2DB: Mining and Identification of Disulfide Bonds in Proteins Utilizing Mass Spectrometric Data,"  Proceedings 55th ASMS Conference on Mass Spectrometry and Allied Topics,  2007, 

Lee, T.; Singh, R.; Yen, T.Y. and Macher, B..  "MS2DB: Algorithms for the Mass Spectrometric Identification of Disulfide Linkage Patterns in Protein,"  IEEE International Symposium on Computer-Based Medical Systems (CBMS),  2007,  p. 397-402.

Lee, T.; Singh, R.; Yen, T.Y.; and Macher, B..  "An Algorithmic Approach to Automated High-Throughput Identification of Disulfide Connectivity in Proteins Using Tandem Mass Spectrometry,"  Computational Systems Bioinformatics Conference,  2007,  p. 41-51.

Macher, B.A. and Yen, T.Y..  "Proteins at membrane surfaces-a review of approaches,"  Molecular BioSystems,  v.3,  2007,  p. 705-713.

McDonald, C.A.; Yang, J.Y.; Yen, T.Y. and Macher, B.A..  "Identifying Cell Surface Glycoproteins using Hydrazide Chemistry in Combination with 2D-LC/ESI-MS/MS,"  USHUPO 4th Annual Conference,  2008, 


(Showing: 1 - 5 of 5).

Please report errors in award information by writing to: awardsearch@nsf.gov.