Award Abstract #0521688
MRI/RUI Acquisition of a Liquid Chromatograph-Mass Spectrometer to Support Chemistry Research at California State University, Northridge

NSF Org:	CHE Division of Chemistry
Initial Amendment Date:	August 5, 2005
Latest Amendment Date:	August 5, 2005
Award Number:	0521688
Award Instrument:	Standard Grant
Program Manager:	Carlos A. Murillo CHE Division of Chemistry MPS Directorate for Mathematical & Physical Sciences
Start Date:	August 15, 2005
Expires:	July 31, 2008 (Estimated)
Awarded Amount to Date:	$336720
Investigator(s):	Eric Kelson eric.kelson@csun.edu (Principal Investigator) Sheri Lillard (Co-Principal Investigator) Paul Shin (Co-Principal Investigator)
Sponsor:	The University Corporation, Northridge 18111 Nordhoff Street Northridge, CA 91330 / -
NSF Program(s):	MAJOR RESEARCH INSTRUMENTATION
Field Application(s):
Program Reference Code(s):	BIOT, 9229, 9184, 9141
Program Element Code(s):	1189

ABSTRACT

With this award from the Major Research Instrumentation (MRI) Program, the Department of Chemistry at California State University in Northridge will acquire a liquid chromatograph ion trap tandem mass spectrometer (LC/MS). Five areas of research will immediately benefit from the requested LC-MS instrumentation: (1) Direct design of transfer hydrogenation catalysts. LC-MS will be used in confirmation of key reaction intermediates (especially ruthenium hydrides and catalyst-substrate adducts) as well as characterization of new catalysts and the products they produce. (2) Understanding fundamental molecular processing in organic sonochemistry. LC-MS will facilitate the determination of intermediate structures from thermal and hydroxyl radical pathways and distinction of the underlying mechanisms. (3) Aromatase-induced metabolism of poly-functional 1,5-alkadiyne anti-tumor agents. LC-MS will be important to confirm aromatase-drug interactions, identify metabolites and test pro-drug designs for targeted delivery of promising agents in breast cancer. (4) Simultaneous analysis of mRNA and protein. LC-MS will be used in the separation and identification of target proteins (beginning with estrogen receptor in breast cancer cells) from bulk lysate samples. (5) Evaluation of the extent and sites of apolipoprotein E glycation. LC-MS will be used to confirm fructoselysine residues within proteolytic fragments of apolipoprotein E secreted from THP-1 macrophages at elevated glucose concentrations.

Liquid chromatography with mass spectrometric detection (LC/MS) is an extremely powerful technique used for the separation and analysis of complex mixtures. This instrument will substantially strengthen scientific research at California State University in Northridge, a primarily undergraduate institution. These studies will have a solid impact in the area of biochemistry and medicinal chemistry.

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