Robyn E. Hannigan
DBI Division of Biological Infrastructure
BIO Directorate for Biological Sciences
Start Date:
September 1, 2005
Expires:
August 31, 2008 (Estimated)
Awarded Amount to Date:
$140290
Investigator(s):
Marjorie Matocq matomarj@isu.edu (Principal Investigator)
Michael Thomas (Co-Principal Investigator)
Sponsor:
Idaho State University
921 South 8th Ave., Stop 8046
pocatello, ID 83209 208/282-2592
NSF Program(s):
MAJOR RESEARCH INSTRUMENTATION
Field Application(s):
Program Reference Code(s):
BIOT, 9184
Program Element Code(s):
1189
ABSTRACT
This project will expand the sequencing capacity at Idaho State University's (ISU) Molecular Re-search Core Facility (MRCF) through the upgrade of their current DNA sequencer and purchase of a robotics unit and a bioinformatics integration package. The MRCF has experienced enor-mous growth in recent years due to increased use of molecular tools by existing faculty and the arrival of six new tenure-track and one research faculty in the Department of Biological Sciences in fall 2004. The acquired instruments will enhance research activities and funding competitive-ness, especially for interdisciplinary collaborative projects. Faculty hires in recent years have been designed to create interactive, interdisciplinary research clusters to plan large-scale pro-jects. Some of these clusters depend upon an efficient, high-capacity MRCF, including evolu-tionary genomics, neurobiology, "extragenomics" (studying plasmids & phages), metagenomics (environmental biodiversity assessment and monitoring), and ecological genomics groups. Syn-ergy within these groups, in addition to individual research efforts, is leading to a groundswell of demand for molecular research capacity.
This instrumentation will take the MRCF in two critical new directions. First, in addition to tra-ditional molecular research, many current users are expanding their investigations to genome-scale analyses. Expansion of the MRCF will contribute to its use in small-genome research. The second critical direction for the new instrumentation involves the integration of the MRCF with ISU's existing bioinformatics facilities. This brings the MRCF into the post-genomic era, allow-ing MRCF users to have a seamless experience from job submission to data quality control and storage to manipulation and analysis. The bioinformatics group at ISU has been working with the MRCF since spring 2004 to do the groundwork for this integration and will continue to play a major role in collaborating with the MRCF and its users.
ISU has long emphasized hands-on research opportunities for undergraduate students, many of whom go on to graduate school in the nation's top graduate programs. The MRCF plays a large role in providing facilities for the recruitment and training of regional Hispanic, Native Ameri-can, and rural students by contributing to a "pipeline to higher education" focused on bringing molecular science experiences into the classroom and students into research laboratories. Initia-tives include the Native Scholars Program for regional native high school students and the Biol-ogy Youth Research Program for local high school students. These outreach programs will ex-pand with the augmentation of MRCF resources.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH Next
(Showing: 1 - 20 of 22).
Battiprolu PK., Harmon KJ, Rodnick KJ..
"Sex differences in energy metabolism and performance of teleost cardiac tissue,"
American Journal of Physiology,
v.292,
2007,
p. R827.
Bearden, S.E..
"Advancing age produces sex differences in vasomotor Kinetics during and after skeletal muscle contraction,"
American Journal of Physiology,
v.293,
2007,
p. R1274.
DeVeaux, L.C., J.A. Müller, J.R. Smith, D.P. Wells, J.E. Petrisko, S. DasSarma.
"Extremely radiation-resistant mutants of a halophilic archaeon with increased single-stranded DNA binding protein (RPA) gene expression,"
Radiation Research,
v.168,
2007,
p. 507.
DeVeaux, LC; Wells, DP; Hunt, A; Webb, T; Beezhold, W; Harmon, JF.
"Accelerator-based radiation sources for next-generation radiobiological research,"
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT,
v.562,
2006,
p. 981
- 984.
Fang, Y. and Olah, M.E..
"cAMP-dependent, Protein Kinase A-independent activation of extracellular signal-regulated kinase 1/2 (ERK1/2) following adenosine receptor stimulation in human umbilical vein endothelial cells ? Role of Epac1,"
. J. Pharmacol. Exp. Ther,
v.322,
2007,
p. 1189.
Germino, M.J., N. J. Hasselquist, T. M. McGonigle, W. K. Smith, P. Sheridan.
"Colonization of conifer seedling roots by fungal mycelium in an alpine- treeline ecotone: Relationships to microsite, developmental stage, and ecophysiology of seedlings.,"
Canadian Journal of Forest Research,
v.36,
2006,
p. 901.
Gerrish, R.S., J.E. Lee, J. Reed, J. Williams, L.D. Farrell, K.M. Spiegel, P.P. Sheridan and M.S. Shields.
"Evaluation of PCR Versus Hybridization for Detection of Plasmid and Chromosomally Encoded Virulence Genes of Representative EHEC Type-Strains.,"
Emerging Infectious Diseases,
v.13,
2007,
p. 1253.
Groome JR, Dice MC, Fujimoto E, Ruben PC..
"Charge immobilization of skeletal muscle Na+ channels: role of residues in the inactivation linker.,"
Biophys J.,
v.93,
2007,
p. 1519.
Horton, Rene' N., William A. Apel, Vicki S. Thompson, Peter P. Sheridan.
"Low temperature reduction of hexavalent chromium by a microbialenrichment consortium and a novel strain of Arthrobacter aurescens,"
BMC Microbiology,
v.6,
2006,
p. 5.
Johnson, DA; Hill, JP; Thomas, MA.
"The monosaccharide transporter gene family in land plants is ancient and shows differential subfamily expression and expansion across lineages,"
BMC EVOLUTIONARY BIOLOGY,
v.6,
2006,
Keeley, E. R., E. A. Parkinson, and E. B. Taylor.
"Ecotypic differentiation of native rainbow trout (Oncorhynchus mykiss) populations from British Columbia.,"
Canadian Journal of Fisheries and Aquatic Sciences,
v.62,
2005,
p. 1523.
Keeley, E. R., E. A. Parkinson, and E. B. Taylor..
"The origins of ecotypic variation of rainbow trout (Oncorhynchus mykiss): an experimental assessment of environmental versus genetically based differences in morphology.,"
. Journal of Evolutionary Biology,
v.20,
2007,
p. 725.
Ledbetter, R.N., S.A. Connon, A.L. Neal, A. Dohnalkova, and T.S. Magnuson.
"Biogenic mineral production by a novel arsenic-metabolizing thermophilic organism from the Alvord Basin, OR,"
Appl. Environ. Microbiol,
v.73,
2007,
p. 5928.
Lee, M.H., J.L. Keams, D.W Helzer, O.P. Leiser, M.A. Ochoa, S.A. Connon, T.S. Magnuson, and M.E. Watwood.
"Characterization of Viral and Prokaryotic Communities in Alvord Desert Hot Springs, Oregon,"
Aqua. Microb. Ecol,
v.48,
2007,
p. 19.
Matocq, M.D., C.R. Feldman, and Q. Shurtliff.
"Phylogenetic relationships of the woodrat genus Neotoma (Rodentia: Muridae): implications for the evolution of phenotypic variation in male external genitalia.,"
Molecular Phylogenetics and Evolution,
v.42,
2007,
p. 637.
Puli, S; Lai, JCK; Edgley, KL; Daniels, CK; Bhushan, A.
"Signaling pathways mediating manganese-induced toxicity in human glioblastoma cells (U87),"
NEUROCHEMICAL RESEARCH,
v.31,
2006,
p. 1211
- 1218.
Seiler, S. M. and E. R. Keeley..
"Morphological and swimming stamina differences between Yellowstone cutthroat trout, rainbow trout, and their hybrids.,"
Canadian Journal of Fisheries and Aquatic Sciences,
v.64,
2007,
p. 127.
Sestero, C.M. and G. M. Scalarone.
"Detection of the surface antigens BAD1 and a-(1,3)-glucan in six different strains of Blastomyces dermatitidis using monoclonal antibodies,"
Journal of Medical and Biological Sciences,
v.1,
2006,
p. 1.
Shurley, J. F., A. M. Legendre and G. M. Scalarone.
"Blastomyces dermatitidis Antigen Detection in Urine Specimens From Dogs With Blastomycosis Using a Competition Binding Inhibition ELISA,"
Mycopathologia,
v.160,
2006,
p. 137.
Shurley, Jack F. and Gene M. Scalarone.
"Comparison of Blastomyces dermatitidis Yeast Phase Lysate Antigens for Their Use as Diagnostic Reagents in the Competitive Inhibition ELISA for the Detection of Blastomycosis,"
Journal of Medical and Biological Sciences,
v.1,
2007,
p. 1.
