Robyn E. Hannigan
DBI Division of Biological Infrastructure
BIO Directorate for Biological Sciences
Start Date:
September 1, 2005
Expires:
August 31, 2008 (Estimated)
Awarded Amount to Date:
$1539360
Investigator(s):
Ruth Welti welti@ksu.edu (Principal Investigator)
Scot Hulbert (Co-Principal Investigator) John Tomich (Co-Principal Investigator) Michael Kanost (Co-Principal Investigator) Frank Blecha (Co-Principal Investigator)
Sponsor:
Kansas State University
2 FAIRCHILD HALL
MANHATTAN, KS 66506 785/532-6804
NSF Program(s):
MAJOR RESEARCH INSTRUMENTATION
Field Application(s):
Program Reference Code(s):
BIOT, 9184
Program Element Code(s):
1189
ABSTRACT
A grant has been awarded to Kansas State University under the direction of Dr. Ruth Welti for partial support of acquisition of analytical instruments that will be utilized by scientists working in the areas of plant metabolism and signaling systems in animals. While, in the past decade the complete sequences of many plant and animal genomes have been elucidated, the function of many genes and their protein products are not fully understood. Two approaches to understanding gene function (functional genomics) are (1) proteomics, in which the protein gene products carrying out specific cellular functions are identified and characterized, and (2) metabolomics, in which metabolites, formed by the action of the protein gene products, are identified and quantified. Two branches of metabolomics are glycomics, which deals with analysis of carbohydrate metabolites, and lipidomics, which deals with lipid metabolite analysis. The characterization of both protein gene products and most metabolites can be best accomplished by mass spectrometry, a technique in which biomolecules are ionized and identified by the masses of molecular ions and their derivative ions. The goal of this project is to acquire four mass spectrometers at Kansas State University with which to accomplish this research. These instruments will be utilized for functional genomics by users at Kansas State University and elsewhere.
The users of the requested instrumentation work in two major areas of functional genomics, plant metabolism and signaling systems in animals. The plant scientists are investigating gene expression and function during plant abiotic and biotic stress and during development. The animal scientists are utilizing mass spectrometric strategies to understand the interplay and roles of proteins and lipids in lipoprotein complexes, membrane lipid rafts, membrane channel function, and immune responses. Acquisition of the mass spectrometers will open doors for new ways of determining the functions of biomolecules, enhancing both scientific and technical training. Formal and informal training on the utility and use of the new instrumentation will be offered to instrument users, including technical staff, undergraduate students, graduate students, postdoctoral trainees, and faculty at all levels.
Acquisition of these mass spectrometers will broaden and improve the training and research capabilities of undergraduate students, graduate students, postdoctoral students, and faculty. The availability of these instruments will enhance and strengthen the research programs of life scientists in the Colleges of Arts and Sciences, Agriculture, Human Ecology, and Veterinary Medicine at Kansas State University, and scientists at other institutions in the United States and around the world. Use of the instruments should lead to greater understanding of gene product functions, of the roles of lipids, and of interactions between proteins and lipids in plants and animals. Making mass spectrometry instrumentation available for fundamental research in chemistry and biology will lead to practical applications in industry, medicine, and agriculture.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH Next
(Showing: 1 - 20 of 23).
Bartz, Rene, Li, Wen-Hong, Venables, Barney, Zehmer, John, Welti, Ruth, Anderson, Richard, Liu, Ping-sheng, and Chapman, Kent D..
"Lipidomics reveals adiposomes store ether lipids and mediate phospholipid traffic.,"
J. Lipid Res.,
v.48,
2007,
p. 837.
Botté, Cyrille, Saïdani, Nadia, Mondragon, Ricardo, Gonzales, Sirenia, Isaac, Giorgis, Mui, Ernest, McLeod, Rima, Dubremetz, Jean-François, Vial, Henri, Welti, Ruth, Cesbron-Delauw, Marie-France, Mercier, Corinne, and Maréchal, Eric..
"Subcellular localization and dynamics of a digalactolipid-like lipid along the life cycle of the apicomplexan parasite Toxoplasma gondii.,"
J. Lipid Res.,
v.49,
2008,
p. 746.
Chaturvedi, Ratnesh, Krothapalli, Kartikeya, Makandar, Ragiba, Nandi, Ashis, Sparks, Alexis, Roth, Mary, Welti, Ruth, and Shah, Jyoti..
"Plastid ω3-fatty acid desaturase-dependent accumulation of a systemic acquired resistance inducing activity in petiole exudates of Arabidopsis thaliana is independent of jasmonic acid.,"
Plant J.,
v.54,
2008,
p. 106.
Chen, J, J. J. Burke, Z. Xin, C. Xu, and J. Velten..
"Characterization of the Arabidopsis thermosensitive mutant atts02 reveals an important role for galactolipids in thermotolerance.,"
Plant, Cell and Environment,
v.29,
2006,
p. 1437.
Cruz-Ramirez A, Oropeza-Aburto A, Razo-Hernandez F, Ramirez-Chavez E, Herrera-Estrella L..
"Phospholipase DZ2 plays an important role in extraplastidic galactolipid biosynthesis and phosphate recycling in Arabidopsis roots.,"
Proc. Natl. Acad. Sci. USA,
v.103,
2006,
p. 6765.
Devaiah, SP; Pan, XQ; Hong, YY; Roth, M; Welti, R; Wang, XM.
"Enhancing seed quality and viability by suppressing phospholipase D in Arabidopsis,"
PLANT JOURNAL,
v.50,
2007,
p. 950
- 957.
Esch, Steven Wynn, Tamura, Pamela, Sparks, Alexis A., Roth, Mary R., Devaiah, S. P., Heinz, E., Wang, X., Williams, Todd D., and Welti, Ruth..
"Rapid characterization of fatty acyl composition of complex lipids by collision-induced dissociation time-of-flight mass spectrometry.,"
J. Lipid Res.,
v.48,
2007,
p. 235.
Fitzgerald, Michael L., Xavier, Ramnik, Haley, Kathleen J., Welti, Ruth, Goss, Julie L., Brown, Cari E., Zhuang, Debbie, Bell, Susan A., Lu, Naifang, Mckee, Mary, Seed, Brian, and Freeman, Mason W..
"ABCA3 inactivation in mice causes respiratory failure, loss of pulmonary surfactant and depletion of lung phosphatidylglycerol.,"
J. Lipid Res.,
v.48,
2007,
p. 621.
Frank, EE; Travers, SE; Hulbert, SH; Leach, JE; Bai, J; Schnable, PS; Smith, MD; Welti, R; Bremer, DJ; Garrett, KA.
"Rust and drought effects on gene expression and phytohormone concentration in the dominant species of tallgrass prairie,"
PHYTOPATHOLOGY,
v.97,
2007,
p. S36
- S36.
Fritz, Markus, Lokstein, Heiko, Hackenberg, Dieter, Welti, Ruth, Roth, Mary, Zähringer, Ulrich, Fulda, Martin, Hellmeyer, Wiebke, Ott, Claudia, Wolter, Frank P., and Heinz, Ernst..
"Chanelling of eukaryotic diacylglycerol into the biosynthesis of plastidial phosphatidylglycerol.,"
J. Biol. Chem.,
v.282,
2007,
p. 4613.
Hong, Yueyun, Pan, Xiangqing, Welti, Ruth, and Wang, Xuemin..
"Alterations of phospholipase Dá3 change Arabidopsis response to salinity and water deficits.,"
Plant Cell,
v.20,
2008,
p. 803.
Li, Weiqi, Wang, Ruiping, Li, Maoyin, Li, Lixia, Wang, Chuanming, Welti, Ruth, and Wang, Xuemin..
"Differential degradation of extraplastidic and plastidic lipids during freezing and post-freezing recovery in Arabidopsis thaliana.,"
J. Biol. Chem.,
v.283,
2008,
p. 461.
Maeda, Hiroshi, Sage, Tammy L., Isaac, Giorgis, Welti, Ruth, and DellaPenna, Dean..
"Tocopherols modulate extra-plastidic polyunsaturated fatty acid metabolism in Arabidopsis at low temperature.,"
Plant Cell,
v.20,
2008,
p. 452.
Moreau, Robert A., Doehlert, Douglas C., Welti, Ruth, Isaac, Giorgis, Roth, Mary, Tamura, Pamela, and Nuñez, Alberto..
"The identification of mono-, di-, tri-, and tetragalactosyl-diacylglycerols and their natural estolides in oat kernels.,"
Lipids,
v.43,
2008,
p. 533.
Pan, Xiangqing, Welti, Ruth, and Wang, Xuemin..
"Simultaneous quantification of phytohormones and related metabolites in crude plant extracts by liquid chromatography-electrospray tandem mass spectrometry.,"
Phytochemistry,
v.69,
2008,
p. 1773.
Philip D. Bates, John B. Ohlrogge, and Mike Pollard.
"Incorporation of Newly Synthesized Fatty Acids into Cytosolic Glycerolipids in Pea Leaves Occurs via Acyl Editing,"
J. Biol. Chem.,
v.282,
2007,
p. 31206.
Qin-Fang Chen, Shi Xiao, and Mee-Len Chye.
"Overexpression of the Arabidopsis 10-Kilodalton Acyl-Coenzyme A-Binding Protein ACBP6 Enhances Freezing Tolerance,"
Plant Physiology,
v.148,
2008,
p. 304.
Regente, M, Corti Monzón, G., and de la Canal, L..
"Phospholipids are present in extracellular fluids of imbibing sunflower seeds and are modulated by hormonal treatments.,"
J. Exp. Botany,
v.59,
2008,
p. 553.
Welti, R; Mui, E; Sparks, A; Wernimont, S; Isaac, G; Kirisits, M; Roth, M; Roberts, CW; Botte, C; Marechal, E; McLeod, R.
"Lipidomic analysis of Toxoplasma gondii reveals unusual polar lipids,"
BIOCHEMISTRY,
v.46,
2007,
p. 13882
- 13890.
Welti, Ruth, Shah, Jyoti, Li, Weiqi, Li, Maoyin, Chen, Junping, Burke, John J., Fauconnier, Marie-Laure, Chapman, Kent, Chye, Mee-Len, and Wang, Xuemin..
"Plant lipidomics: Discerning biological function by profiling plant complex lipids using mass spectrometry.,"
Frontiers in Bioscience,
v.12,
2007,
p. 2494.
