Award Abstract #0722688
MRI: Instrument Acquisition to Enable Time-Resolved Single-Molecule Fluorescence Measurements at Wheaton College
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NSF Org: |
CHE
Division of Chemistry
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Initial Amendment Date: |
August 10, 2007 |
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Latest Amendment Date: |
August 10, 2007 |
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Award Number: |
0722688 |
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Award Instrument: |
Standard Grant |
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Program Manager: |
Carlos A. Murillo
CHE Division of Chemistry
MPS Directorate for Mathematical & Physical Sciences
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Start Date: |
August 15, 2007 |
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Expires: |
July 31, 2010 (Estimated) |
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Awarded Amount to Date: |
$385762 |
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Investigator(s): |
Daniel Burden daniel.l.burden@wheaton.edu (Principal Investigator)
Lisa Keranen Burden (Co-Principal Investigator) Peter Walhout (Co-Principal Investigator) Jennifer Busch (Co-Principal Investigator)
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Sponsor: |
Wheaton College
501 College Avenue
Wheaton, IL 60187 630/752-5833
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NSF Program(s): |
MAJOR RESEARCH INSTRUMENTATION
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Field Application(s): |
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Program Reference Code(s): |
OTHR,9178,0000
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Program Element Code(s): |
1189
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ABSTRACT
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The Chemistry Department at the Wheaton College will acquire a cluster of instruments to make their single-molecule microscopy and laser laboratory at Wheaton College capable of picosecond to nanosecond time-resolved fluorescence measurements. A high-power, picosecond-pulsed laser will be configured for multi-user access with data acquisition hardware that includes: (1) a time correlated single photon counting (TCSPC) PC card; (2) a stage, interface, and software for fluorescence lifetime imaging (FLIM); (3) a fluorescence lifetime spectrometer; (4) electronics and software for time-gated optical and electrical detection of single ion channels in large lipid bilayer membranes. The specific research contributions facilitated by this instrumentation request include: (1) the development of a novel time-gated detection scheme that will expand the detection area for single molecules in the far-field by ~100-fold over the existing state-of-the-art technique; (2) a comparison of the dynamics and structure of alpha hemolysin and Anthrax toxins in lipid membranes; (3) a study of electron transfer and solvation dynamics in polyelectrolyte multilayer (PEM) thin films; (4) the development of new time-resolved fluorescence assays for structure/function studies of cGMP-dependent protein kinase.
Fluorescence instrumentation provides an excellent means for fostering interdisciplinary scientific investigation because fluorescence applications are replete throughout chemistry, biology, and physics. Fluorescence measurements record the emission of radiation after being probed with a laser or light source. They are inherently sensitive, enabling trace amounts of material to be probed. Once established, the techniques are relatively simple and robust, which facilitates multi-user accessibility. The equipment will be used by undergraduates in research and laboratory instruction.
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