Helen G. Hansma
DBI Division of Biological Infrastructure
BIO Directorate for Biological Sciences
Start Date:
July 15, 2003
Expires:
June 30, 2007 (Estimated)
Awarded Amount to Date:
$487352
Investigator(s):
Edward Dratz dratz@chemistry.montana.edu (Principal Investigator)
Mark Young (Co-Principal Investigator) Charles Paden (Co-Principal Investigator) Timothy McDermott (Co-Principal Investigator) Anne Camper (Co-Principal Investigator)
Sponsor:
Montana State University
309 MONTANA HALL
BOZEMAN, MT 59717 406/994-2381
NSF Program(s):
MAJOR RESEARCH INSTRUMENTATION
Field Application(s):
Program Reference Code(s):
BIOT, 9184
Program Element Code(s):
1189
ABSTRACT
A grant has been awarded to Montana State University under the direction of Dr. Edward Dratz from the Department of Chemistry and Biochemistry for the acquisition of proteomics equipment for research and training. The dawn of the post-genomic era is leading to extraordinary opportunities and terrific challenges for understanding fundamental processes in biology. A central question is how the various gene products (primarily proteins) work together to accomplish biological functions and allow the organisms to adapt to changing circumstances. The genome of an organism is quite static (apart from rare mutations), whereas the proteins expressed by the cells in organisms are highly dynamic and change rapidly in response to stimuli. Proteomics is the study of the proteins that are up- or down-regulated or changed in post-translational modifications (PTMs) in response to biological or environmental stimuli. Typical proteins may have many different PTMs, which usually affect the protein's activity and/or cellular localization.
Ten groups of investigators from five departments (Chemistry and Biochemistry, Plant Sciences, Land Resources and Environment, Microbiology and Cell Biology and Neurosciences) and the Center for Biofilm Engineering at Montana State University will make heavy use of the proteomics equipment and proteomics training capability that will be provided by this grant. The projects cut across many fields, including adaptation of hyperthermophilic archaea to extreme environments, development of new, ultrasensitive multiplex detection methods in functional proteomics, mechanisms of biofilm formation in marine bacteria and regulatory pathways in biofilm control, two different studies of neurodevelopment in chick and xenopus, and mechanism of axonal sprouting in the rat brain.
One crucial outcome from acquisition of the equipment and support is broad cross-training opportunities for postdoctorals and for students at many levels in the use of advanced proteomics methodology. Montana State University has a long history of combining research and teaching and strongly integrating undergraduate students into research laboratories during the academic year and during the summer (with several REU Programs). Two of the REU programs are designed to attract Native American students from Tribal Colleges in Montana.
PUBLICATIONS PRODUCED AS A RESULT OF THIS RESEARCH
(Showing: 1 - 6 of 6).
Angel, TE; Kraft, PC; Dratz, EA.
"Metarhodopsin-II stabilization by crosslinked Gt(alpha) C-terminal peptides and implications for the mechanism of GPCR-G protein coupling,"
VISION RESEARCH,
v.46,
2006,
p. 4547
- 4555.
Barry, RC; Young, MJ; Stedman, KM; Dratz, EA.
"Proteomic mapping of the hyperthermophilic and acidophilic archaeon Sulfolobus solfataricus P2,"
ELECTROPHORESIS,
v.27,
2006,
p. 2970
- 2983.
Maaty, WSA; Ortmann, AC; Dlakic, M; Schulstad, K; Hilmer, JK; Liepold, L; Weidenheft, B; Khayat, R; Douglas, T; Young, MJ; Bothner, B.
"Characterization of the archaeal thermophile Sulfolobus turreted icosahedral virus validates an evolutionary link among double-stranded DNA viruses from all domains of life,"
JOURNAL OF VIROLOGY,
v.80,
2006,
p. 7625
- 7635.
Piscitelli, CL; Angel, BE; Bailey, BW; Hargrave, P; Dratz, EA; Lawrence, CM.
"Equilibrium between metarhodopsin-I and metarhodopsin-II is dependent on the conformation of the third cytoplasmic loop,"
JOURNAL OF BIOLOGICAL CHEMISTRY,
v.281,
2006,
p. 6813
- 6825.
Taylor, RM; Baniulis, D; Burritt, JB; Gripentrog, JM; Lord, CI; Riesselman, MH; Maaty, WS; Bothner, BP; Angel, TE; Dratz, EA; Linton, GF; Malech, HL; Jesaitis, AJ.
"Analysis of human phagocyte flavocytochrome b(558) by mass spectrometry,"
JOURNAL OF BIOLOGICAL CHEMISTRY,
v.281,
2006,
p. 37045
- 37056.
Taylor, RM; Maaty, WSA; Lord, CI; Hamilton, T; Burritt, JB; Bothner, B; Jesaitis, AJ.
"Cloning, sequence analysis and confirmation of derived gene sequences for three epitope-mapped monoclonal antibodies against human phagocyte flavocytochrome b,"
MOLECULAR IMMUNOLOGY,
v.44,
2007,
p. 625
- 637.
(Showing: 1 - 6 of 6).
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