Chemical Sampling Information
	4,4'-Methylene-bis(2-Chloroaniline)
Quick Links General Description Exposure Limits Health Factors Monitoring Search    [use word(s)/phrase] Table of Contents By Name A B C D E F G H I J K L M N O P Q R S T U V W X Y Z By CAS Number General Description Synonyms: DACPM; 3,3'-Dichloro-4,4'-diaminodiphenylmethane; MBOCA; 4,4'-Methylenebis(o-chloro aniline); 4,4'-Methylenebis(2-chlorobenzenamine); MOCA OSHA IMIS Code Number: 2650 Chemical Abstracts Service (CAS) Registry Number: 101-14-4 NIOSH, Registry of Toxic Effects (RTECS) Identification Number: CY1050000 NIOSH Pocket Guide to Chemical Hazards, 4,4'-Methylene-bis(2-chloroaniline): chemical description, physical properties, potentially hazardous incompatibilities, and more Exposure Limits American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value (TLV): 0.01 ppm, 0.11 mg/m3 TWA; Skin; Appendix A2 - Suspected Human Carcinogen; BEI National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Limit (REL): 0.003 mg/m3 TWA; Skin; Appendix A - NIOSH Potential Occupational Carcinogens Health Factors National Toxicology Program (NTP) carcinogenic classification: Reasonably Anticipated to be Human Carcinogen [228 KB, PDF] International Agency for Research on Cancer (IARC) carcinogenic classification: Group 2A, probably carcinogenic to humans [104 KB, PDF] Potential symptoms: Hematuria (blood in the urine), cyanosis, nausea, methemoglobinemia, headache, dizziness, confusion, convulsions; kidney irritation; [potential occupational carcinogen] Health Effects: Carcinogen (HE1); Mutagen (HE2); Methemoglobinemia (HE13). Affected organs: Blood, liver, kidneys Notes: 4,4’-Methylene-bis(2-chloroaniline) (MBOCA) is an OSHA Select Carcinogen without a PEL. It may be absorbed through the skin and cause a burning sensation. It has been suggested that measuring urinary MBOCA, perhaps after hydrolysis of a glucuronide conjugate, is a better index of exposure than monitoring airborne MBOCA because of significant skin absorption. A case of bladder cancer was diagnosed in an employee exposed 14 years during the manufacture of this chemical. Air samples taken two consecutive days in his work area indicated MBOCA concentrations of 0.23 and 0.41 mg/m3. N-hydroxy- and mononitroso-derivatives of MBOCA, but not MBOCA itself, were reported to produce adducts with rat and human hemoglobin in vitro. Date Last Revised: 09/01/2006 Literature Basis: NIOSH Pocket Guide to Chemical Hazards: 4,4'-Methylene-bis(2-chloroaniline). International Chemical Safety Cards (WHO/IPCS/ILO): 4,4’-Methylene bis(2-chloroaniline). Chen, T.H., Kuslikis, B.I. and Braselton, W.E., Jr.: Unlabeled hemoglobin adducts of 4,4’-methylenebis(2-chloroaniline) in rats and guinea pigs. Arch. Toxicol. 65(3): 177-185, 1991. Cocker, J., Boobie, A.R., Wilson, H.K. and Gompertz, D.: Evidence that a beta-N-glucuronide of 4,4’-methylenebis(2-chloroaniline) (MbOCA) is a major urinary metabolite in man: implications for biological monitoring. Br. J. Ind. Med. 47(3): 154-161, 1990. Ichikawa, Y., Yoshida, M., Okayama, A., Hara, I. and Morimoto, K.: Biological monitoring for workers exposed to 4,4’-methylenebis(2-chloroaniline). Am. Ind. Hyg. Assoc. J. 51(1): 5-7, 1990. Liu, C.-S., et al.: Occupational bladder cancer in a 4,4’-methylenebis(2-chloroaniline) (MBOCA)-exposed worker. Environ. Health Perspec. 113(6): 771-774, 2005. No Author: 4,4’-Methylenebis(2-Chloroaniline), CAS No. 101-14-4. Report on Carcinogens, Eleventh Edition; U.S. Department of Health and Human Services, Public Health Service, National Toxicology Program, 2005. [228 KB, PDF] Pohanish, R.P. (editor): 4,4’-Methylenebis(2-Chloroaniline). In, Sittig’s Handbook of Toxic and Hazardous Chemicals and Carcinogens, Fourth Ed., Vol. 2. Norwich, NY: Noyes Publications, William Andrew Publishing, 2002, pp. 1553-1556. Monitoring Methods used by OSHA Laboratory Sampling/Analytical Method: sampling media: Coated Glass Fiber Filters (37 mm) in 3 piece cassette. Coating is Sulfuric Acid. maximum volume: 100 Liters   maximum flow rate: 1.0 L/min current analytical method: Gas Chromatography; GC/ECD method reference: OSHA Analytical Method (OSHA 71) method classification: Fully Validated note: Within ten hours after sampling, transfer filter to glass vial containing 2 mL deionized water. Filters may be obtained by OSHA personnel from SLTC. Wipe Sampling Method: sampling media: Glass Fiber Filter note: Place the wipe sample into a glass vial containing 2 mL of 0.01 N Sulfuric Acid. Bulk method: Limit the amount of bulk submitted to one gram or one mL. All Trademarks are the property of their respective owners. Accessibility Assistance: Contact the OSHA Directorate of Science, Technology and Medicine at 202-693-2300 for assistance accessing PDF materials.