Bryon Donohoe - Research Scientist
Bryon Donohoe applies his background in structural biology and plant cell biology to study the underpinnings of biomass recalcitrance. In particular, he has turned several years of studying the cellular mechanisms involved in plant cell wall assembly toward now understanding the biological disassembly of plant cell walls in biomass. Dr. Donohoe is a key user and mentor of the Biomass Surface Characterization Laboratory—a collection of imaging technologies including laser scanning confocal microscopy, environmental scanning electron microscopy, atomic force microscopy, and transmission electron microscopy—used to better understand structural changes during biomass conversion. In particular, he is expert at biomass sample preparation including cryopreservation, immuno-EM, and using electron tomography as a technique to study the complex 3-D structure of the cell wall at the molecular scale. Dr. Donohoe is also involved in efforts to understand microbial interactions with biomass and the structure of environmental microbial communities.
Education
- Ph.D., Molecular/Cellular Biology, University of Colorado at Boulder
- M.A., Biology, University of Colorado at Denver
- B.S., Biological Science, Colorado State University
Memberships
- Microscopy Society of America
- American Society of Plant Biologists
Selected Publications
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Donohoe BS, Decker SR, Tucker MP, Himmel ME, Vinzant TB, (2008), "Visualizing lignin coalescence and migration through maize cell walls following thermochemical pretreatment," Biotechnology & Bioengineering (in press).
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Ahrenkiel SP, Yu P, Murphy, JE, Nedeljkovic JM, and Donohoe BS, (2008), "Nanoparticle shape and configuration analysis by transmission electron tomography," Journal of Microscopy (in press).
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Donohoe BS, Kang BH, Gerl M, Gergely Z, Staehelin LA, (2008), "Electron tomographic characterization of cis Golgi cisternal assembly in Scherffelia dubia, Chlamydomonas reinhardtii, and higher plants," Current Biology, (in press).
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Porter SE, Donohoe BS, Beery KE, Xu Q, Ding S-Y, Vinzant TB, Abbas CA, Himmel ME, (2007), "Microscopic analysis of corn fiber using starch- and cellulose-specific molecular probes, Biotechnology & Bioengineering, 98:123-131.
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Donohoe BS, Kang BH, Staehelin LA, (2006), "Identification and characterization of COPIa- and COPIb-type vesicle classes associated with plant and algal Golg,. Proceedings of the National Academy of Sciences (PNAS), 104:163-168.
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Donohoe BS, Mogelsvang S, Staehelin LA, (2006), "Electron tomography of ER, Golgi and related membrane systems," Methods, 39:154-162.
NREL Publications
View NREL publications for this staff member.
Other Team Members
Bryon Donohoe |
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