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A LC/MS Method for the Determination of Cyanobacteria Toxins in Water
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| Abstract: | The cyanobacteria toxins anatoxin-a, microcystin-LR, microcystin-RR, microcystin-YR, and nodularin were separated in less than 30 minutes on several 1 mm x 15 cm reverse phase liquid chromatography (LC) columns, and their electrospray mass spectra were measured with 50 ng or less injected amounts with a bench-top time-of-flight (TOF) mass spectrometer. New data from this work includes: (a) the impact of acetic acid concentrations in the methanol-water mobile phase on measured ion abundances; (b) the performance of the electrospray-TOF mass spectrometer as an LC detector; (c) the accuracy and precision of exact m/z measurements after LC separation with a routinely-used mass spectrometer resolving power of 5000; and, (d) recoveries of the five toxins from reagent water, river waters, and sewage treatment plant effluent samples extractged with C-18 silica particles enmeshed in thin Teflon (trademark) membrane filter disks. This technique has the otential of providing a relatively simple and reasonable-cost sample preparation and LC/MS method that provides the sensitivity, selectivity, reliability, and information content needed for drinking water occurrence and human exposure studies. |
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| Citation: | Maizels, M., and W. L. Budde. A LC/MS Method for the Determination of Cyanobacteria Toxins in Water. ANALYTICAL CHEMISTRY 76(5):1342-1351, (2004). |
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| Contact: |
Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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| Division: |
Microbiological & Chemical Exposure Assessment Division |
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| Branch: |
Immediate Office |
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| Product Type: |
Journal |
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| Published: |
03/01/2004 |
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