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Immunocytochemical Localization of Stachylysin in Stachybotrys Chartarum Spores and Spore-Impacted Mouse and Rat Lung Tissues
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| Abstract: | Stachylysin is a proteinaceous hemolytic agent that is producted by S. chartarum. Stachylysin was found, using immunohistochemistical and immunocytochemical methods, to be localized in S. chartarum spores/mycelia primarily in the inner wall suggesting that it is constitutively produced. Spores instilled in mouse or rat lung tissues resulted in granuloma formations which showed the highest stachylysin concentration in the inner wall of the spore, with less at distance indicating that it has diffused out from the spore. The in vitro high stachylysin producing strain (58-06) was also highest in vivo, based on immunohistochemistical staining. More stachylysin was observed in the lung tissue at 72 h than at 24 h indicating that production /release is a relatively slow process. The localization of stachylysin in macrophage phagolysosomes suggests that these cells may be involved with hemolysin inactivation. This would be consistent with what is know about asp-hemolysin produced by Aspergillus fumigatus. |
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| Citation: | Gregory, L., T. G. Rand, D. Dearborn, I. Yike, and S. J. Vesper. Immunocytochemical Localization of Stachylysin in Stachybotrys Chartarum Spores and Spore-Impacted Mouse and Rat Lung Tissues. MYCOPATHOLOGIA 156(2):109-117, (2003). |
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| Contact: |
Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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| Division: |
Microbiological & Chemical Exposure Assessment Division |
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| Branch: |
Microbial Exposure Research Branch |
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| Product Type: |
Journal |
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| Published: |
03/01/2003 |
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