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Comparison of Five Extraction Methods on Incurred and Fortified Pesticides in Composite Diets: Blender, Soxhlet, Ase, Microwave and Sfe
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| Abstract: | The USEPA National Exposure Research Laboratory studies dietary exposure to a diverse group of semi-volatile pesticides by analyzing 24 hour duplicate composite diets. The pesticides of interest include organochlorines, organophosphates, anilines, and triazines. Currently, there are no official methods for pesticide analysis of composite samples that include fatty and non-fatty foods. Composite diets consisting of multiple food items with field-incurred or laboratory-fortified pesticides were created to compare extraction efficiency by Soxhlet, blender, microwave, ASE, and SFE. Similar pesticide recoveries were obtained by all extraction methods, though the methods varied widely in instrument cost, extraction time, and solvent use. Co-extractants were removed using a novel clean-up method that included pre-separation in the large volume injector of a GC/MS. The extraction, clean-up, and determinative steps permit the analysis of non-polar and moderately polar pesticides in fatty samples with a method detection limit of about 1 ng/g for thermally stable semi-volatile pesticides and reduce solvent consumption. |
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| Citation: | Rosenblum, L. Comparison of Five Extraction Methods on Incurred and Fortified Pesticides in Composite Diets: Blender, Soxhlet, Ase, Microwave and Sfe. Presented at AOAC Central Regional Meeting, Columbus, OH, November 1-3, 2000. |
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| Contact: |
Mary P. O'Bryant - (919)-541-4871 or obriant.mary@epa.gov
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| Division: |
Microbiological & Chemical Exposure Assessment Division |
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| Branch: |
Chemical Exposure Research Branch |
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| Product Type: |
Abstrct/Oral |
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| Presented: |
11/02/2000 |
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