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Toxicogenomics as a Tool to Assess Exposure of Fish to Environmental Pollutants
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| Abstract: | Molecular biological techniques such as gene arrays and quantitative real-time PCR are becoming important tools to study alterations in normal gene expression in fish and other wildlife exposed to such pollutants as endocrine disrupting chemicals (EDCs). An important function for these tools is the ability to translate from the laboratory to field conditions to assess exposure to EDCs and other pollutants in wild fish. Detection of anomalies at the genomic level will enable screening methods to identify toxic effects soon after exposure, at the molecular level, before they are manifested at the tissues, organs, individuals or population level. One area of concern in which in which these methods may be useful is in the assessment of concentrated animal feeding operation (CAFO) effluents on gene expression in fish. The results of this work will not only provide information as to the effects of CAFO effluents on gene expression in fish, but will also be an initial field validation of the fathead minnow gene array currently in development at EERD. It is hoped that the gene array can and will be used by EPA Regions, states, tribes and other monitoring agencies in field applications to screen for EDCs and other pollutants that alter normal gene expression in fish. |
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| Citation: | Knoebl, I. Toxicogenomics as a Tool to Assess Exposure of Fish to Environmental Pollutants. Presented at Science Forum 2004, Washington, DC, June 1-3, 2004. |
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| Contact: |
Linda Ransick - (513) 569-7395 or ransick.linda@epa.gov
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| Division: |
Ecological Exposure Research Division |
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| Branch: |
Molecular Ecology Research Branch |
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| Product Type: |
Abstrct/Oral |
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| Presented: |
06/02/2004 |
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