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Assessing the Use of Oligonucleotide Microarrays for Fathead Minnow (Pimephales Promelas) to Examine Exposure Variables
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| Abstract: | Microarray technology has proven to be a useful tool for analyzing the transcriptome of various organisms representing conditions such as disease states, developmental stages, and responses to chemical exposure. Although most commercially available arrays are limited to organisms that have complete, or near complete, genome information, efforts directed at developing arrays for other model organisms have been gaining attention in an effort to fulfill experrimental and programmatic needs of academia, industry, and regulatory agencies. We describe some of the first experiments examining the use of custom made oligonucleotide arrays developed to assess transcriptome changes in a popular aquatic toxicity model, the fathead minnow (Pimephales promelas). Microarray results for adult tissues from several chemical exposures are presented. Use of guidelines following Microarray Gene Expression Data (MGED) Society standards such as MIAMI (minimal information about microarray experiments) and MAGE (microarry gene expression) for experimental design and validation are described with the perspective of producing data that are useful for environmental studies. |
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| Citation: | Miracle, A. L., I. Knoebl, D. Villeneuve, G. Ankley, N. Denslow, B. Carter, and P. Larkin. Assessing the Use of Oligonucleotide Microarrays for Fathead Minnow (Pimephales Promelas) to Examine Exposure Variables. Presented at Society of Environmental Toxicology & Chemistry, Portland, OR, November 14-18, 2004. |
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| Contact: |
Linda Ransick - (513) 569-7395 or ransick.linda@epa.gov
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| Division: |
Ecological Exposure Research Division |
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| Branch: |
Molecular Ecology Research Branch |
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| Product Type: |
Abstrct/Oral |
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| Presented: |
11/15/2004 |
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