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A Daphnia Magna Short-Term Survival and Growth Test
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| Abstract: | With the change in acceptable test temperatures for invertebrate toxicity tests from <20oC to 25oC, it is now possible to use Daphnia magna for short-term chronic testing. When cultured at 25oC the dry weight of <24 hr old D. magna ranges from 7 to 15 g depending upon nutrition, brood number and/or brood size. Within 48 hours such daphnids increase their dry weight to 40 to 60 ug and within 94 hrs can weigh 100 to 200 ug. This rate of increase in growth makes this organism very desirable for use in a short-term chronic method. The purpose of this study was to develop a 2 to 4 day D. magna short-term survival and growth test that was similar in sensitivity to the 7 day Ceriodaphnia dubia survival and reproduction test. The results thus far, using 3 reference toxicants, indicate that the difference in sensitivity for the two test methods is less than a dilution or factor of 2. |
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| Citation: | Lazorchak, J. M., M. E. Smith, L. E. Herrin, and M. E. Young. A Daphnia Magna Short-Term Survival and Growth Test. Presented at Biological Advisory Committee Meeting, Newport, RI, October 14-16, 2003. |
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| Contact: |
Linda Ransick - (513) 569-7395 or ransick.linda@epa.gov
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| Division: |
Ecological Exposure Research Division |
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| Branch: |
Molecular Ecology Research Branch |
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| Product Type: |
Abstrct/Oral |
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| Presented: |
10/14/2003 |
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