Alexandra Dubini - Postdoc
Alexandra Dubini is investigating the global gene expression of algal cultures under H2 -producing conditions using the microarray technique. She is also studying the maturation process of the [Fe-Fe] enzyme in Chlamydomonas reinhardtii. Prior to coming to NREL, she researched the identification of new chaperones involved in the Tat system at the University of East Anglia, UK, where she received her Ph.D.
Dr. Dubini's skills and experience include biochemistry and molecular biology techniques such as anaerobic protein purification, protein engineering, protein expression, mutagenesis PCR gene cloning, western blotting, immunoprecipitation (pull-down assay), mutagenesis, and rocket immunoelectrophoresis.
Education
| 2001–2004 | Ph.D., Molecular Biology and Biochemistry, School of Biological Sciences University of East Anglia, UK |
| 1999–2001 | B.S. (Hons), Molecular Biology, School of Biological Sciences University of East Anglia, UK |
| 1997–1999 | Diplome d'Etudes Universitaires General Sciences de la Nature et de la Vie University of Mediterranee, Aix-Marseille, France. |
Selected Publications
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Jack R.L., Dubini A., Palmer T., Sargent F., "Common principles in the biosynthesis of diverse enzymes," Biochem Trans, in press.
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Jack R.L., Buchanan G., Dubini A., Hatzixanthis K., Palmer T., Sargent F., "Coordinating assembly and export of complex bacterial proteins," EMBO J 76, 409–406 (2004).
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Dubini A., Sargent S., "Assembly of Tat-dependent [NiFe] hydrogenases: identification of precursor-binding accessory proteins," FEBS Lett 549, 141–146 (2003).
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Dubini A., Pye R., Jack R., Palmer T., Sargent F., "How bacteria get energy from hydrogen: a genetic analysis of periplasmic hydrogen oxidation in Escherichia coli.," Int. J. Hydrogen Energy 27, 1413–1420 (2002).
Other Team Members
Alexandra Dubini |
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