Technical Abstract: Non-invasive measures of stress are needed for experimental purposes and for potential use in well-being audits. Sucrose consumption is frequently used to determine that a stress response has been induced in mice and rats. This study was conducted to determine the effect of experimentally increased glucose demand on the level of consumption of molasses by dairy calves. Ten 3-wk-old calves were randomly assigned to 0.365g of phlorizin in 3 mL of saline by s.c.injection or to 3 mL of saline s.c.injection treaments in a randomized complete block design. Urinary output, urinary glucose levels, and molasses consumption were measured hourly. Molasses consumption for the 24 hours after treatment was greater (P = 0.02) for the phlorizin treated group (1.42g) than for the control group (0.72g). Mean 8-h urinary output was less (P = 0.026) for control calves (1.13 kg) than for phlorizin treated calves (1.67 kg). Mean urinary glucose concentration for control calves remained close to 0 mg/dL during the entire 8-h observation period and was greater (P < 0.001) for the phlorizin treated calves by 2h. Mean urinary glucose was 30 mg/dL for the first 2 h, peaked at 60 mg/dL 4 h post treatment, and ended at 16.6 mg/dL 8 h post treatment. In 3-wk-old Holstein calves, molasses consumption increased significantly due to phlorizin treatment, suggesting that consumption of molasses is related to glucose demand.