Project Number: 1230-21000-055-09
Project Type: Specific Cooperative Agreement

Start Date: Oct 01, 2007
End Date: Sep 30, 2009

Objective:
Sequencing 20,000 cDNAs of creeping and velvet bentgrass obtained from unstressed mature leaves and assign the expressed sequence tags (ESTs) to an existing linkage map of the Agrostis genome, in order to develop an understanding of the genome organization of bentgrass spp. including creeping, colonial, and velvet bentgrass. Comparison of the EST map locations between bentgrass species. Mapping of important traits such as disease resistance using a common set of RFLP anchor probes and the ESTs based on the developed linkage maps.

Approach:
Currently more than 500 clones of two previously developed cDNA libraries from the parents of a creeping bentgrass mapping population have been sequenced. Sequencing reactions of an additional 20,000 cDNAs from two related mapping populations and cDNAs from a diploid Agrostis species velvet bentgrass will be sequenced. Linkage maps of bentgrass spp. will be developed using single nucleotide polymorphism makers identified through sequencing and a set of anchor RFLP probes. QTL mapping of disease resistance will be performed using the maps.