2007 Annual Report 1a.Objectives (from AD-416) The objective of this cooperative research project is to compare and identify pheromone components produced by southern and northern strain plum curculios. 1b.Approach (from AD-416) A headspace collection system at the USDA-ARS Appalachian Fruit Research Station in Kearneysville WV will be used to collect and extract volatiles from southern strain plum curculios. An identical system will be used at St. Hyacinthe, Quebec, Canada to collect and extract volatiles from nothern strain plum curculios. Samples will be sent to USDA-ARS Chemicals Affecting Insect Behavior Laboratory in Beltsville MD for analysis. Experimental lures based on compound identification will be formulated in Beltsville MD, USA and evaluated for biological activity at Kearneysville WV for the southern strain plum curculioand St. Hyacinthe, Quebec, Canada for the northern strain plum curculio. 3.Progress Report This report serves to document progress made under a non-funded cooperative agreement between ARS and IRDA, St. Hyacinthe, Quebec, Canada. Additional details can be found in the report for the parent project 1931-21000-015-00D, Integrated Orchard Management and Automation for Deciduous Tree Fruit Crops. At the end of the first year, a technique has been developed that allows for successful collection volatiles produced by virgin male northern and southern strain plum curculios in the laboratory. Volatiles have been collected from both strains and are being analyzed to confirm the presence of grandisoic acid and other novel pheromone compounds. Further collections will be made based on age and physiological state of the insect. An electroantennogram technique will be modified and incorporated into a gas chromatograph-electroanntegraphic detection system in order to identify stimulating volatiles. The ADODR monitored progress through phone calls, emails, site visits, and regional meetings.