2007 Annual Report 1a.Objectives (from AD-416) To compile a library of spectral fingerprints (combined spectra with no prior separation) and chromatographic profiles for identification and authentification of botanical materials and their extracts. 1b.Approach (from AD-416) Methods will be developed for acquiring spectral fingerprints and chromatographic profiles for botanical materials based on diode array, infrared, near-infrared, and mass spectrometric detection. Pattern recognition software will be used to identify extracts and original plant solids. Appropriate internal standards will be developed to allow for long term comparison of the fingerprints and profiles. 3.Progress Report This report documents research conducted under a reimbursable agreement between ARS and the Office of Dietary Supplements at the National Institutes of Health. Additional details of research can be found in the report for the in-house associated project 1235-52000-048-00D, “Analytical Methods for the Determination of Phenolic Phytonutrients in Foods.” This was the second year of a 3-year project for the development of a library of spectral fingerprints and chromatographic profiles of botanical materials. Botanical materials of significantly different taxonomic and structural origin were examined to establish the natural range of analytical parameters. Ten different botanical materials (black cohosh, echinacea, cranberry, ginseng, grapeseed extract, milk thistle, skullcap, cinnamon, hoodia, and tea) were acquired, based primarily on the priority list established by the Ingredients Ranking Subcommittee of the Presidential Task Force on Dietary Supplements of the Association of Official Analytical Chemists. Authentic as well as commercial materials (raw and processed) were acquired from local sources. Examination the UV and mass spectral fingerprints and liquid chromatographic profiles of the aqueous methanol extracts is in progress. The chromatographic profiles and spectral fingerprints of Ginkgo biloba are being examined in detail. Adulteration of commercial materials is easily detected if individual flavonoids are determined. 5.Significant Activities that Support Special Target Populations None