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Research Project:
GENETIC SEXING IN THE CODLING MOTH: MAPPING INSERTIONS BY FLUORESCENCE IN SITU HYBRIDIZATION (FISH)
Location: Wapato, Washington
Project Number: 5352-22000-017-14
Project Type:
Trust
Start Date: Oct 01, 2003
End Date: Sep 30, 2008
Objective:
Demonstrate repeatability of FISH for detection of single copy genes. Use a single copy gene, period, for marking the sex chromosomes of codling moth. Use B. mori BAC library for mapping chromosomes of codling moth.
Approach:
Demonstrate resolution and repeatability of FISH using single copy gene of approximately 2,400 bp (size of transgene insertion), on codling moth material. Explore the use of period gene as a marker for sex chromosomes. Determine the value of the B. mori BAC library in creating homologous landmarks on chromosomes of codling moth. Documents Trust with International Atomic Energy. Log 25358.
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Last Modified: 11/05/2008
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