2007 Annual Report 1a.Objectives (from AD-416) The objective of this cooperative research project is the study of resistance management and injury potential of Lepidopterous pests to transgenic cottons. 1b.Approach (from AD-416) Collections of feral heliothines are key to assess their susceptibility to Bacillus thuringiensis proteins. Due to the fact that Tennessee Agricultural Landscape is unique among the cotton-producing belt, samples from this region offer a great opportunity to conduct behavioral and ecological research with these pests. This work can lead to a better detection of Bt resistance in feral Lepidoptera as well as the enhancement of the knowledge of heliothine’s reproductive biology. 3.Progress Report This report serves to document research conducted under a Specific Cooperative Agreement between University of Tennessee and ARS. Additional details of research can be found in the report for the in-house project 6402-22000-047-00D, "Resistance Management and Injury Potential of Lepidopterous Pests to Transgenic Cottons." Bollworm and tobacco budworm field populations were collected on a variety of host plants. Progeny were exposed to Cry1Ac, Cry2Ab, and Cry1F toxins in diet incorporated bioassays. No resistance was detected. This project was monitored through various emails and meetings with the cooperator.