2007 Annual Report 1a.Objectives (from AD-416) To determine whether ozone fumigation at 10,000 ppm at -32ml Hg of vacuum for 6 hours will control the uredospores of coffee leaf rust, Hemileia vastatrix. The candidate fumigation schedule was shown to control coffee berry borer, Hypothenemus hampei. If successful in controlling coffee leaf rust uredospores, the ozone fumigation schedule will be recommended as an alternative methyl bromide fumigation for green coffee beans. 1b.Approach (from AD-416) Coffee leaves with sporulating bodies will be collected from fields infected with coffee leaf rust near the Plant Protection and Quarantine Laboratory located in Papeete, Tahiti, French Polynesia, and fumigated in equipment developed by and shipped to Tahiti from Tahoe Food Technologies, Sparks, Nevada. Control and fumigated leaves will be placed in distilled water or on potato agar diet to observe for germination. Germination is scored as viable uredospores; non-germination is scored as dead uredospores. (Documents SCA with Amicale de la Police Phytosanitaire). 3.Progress Report This report serves to document research conducted under a specific cooperative agreement between the USDA-ARS, U.S. Pacific Basin Agricultural Research Center and Amicale De La Police Phytosanitaire, Pape’ete, Tahiti, to provide laboratory space and technical assistance to ARS to develop efficacy data on ozone fumigation under vacuum to control coffee leaf rust uredospores. Additional details of research can be found in the report for the parent CRIS 5320-43000-014-00D, Postharvest Treatment of Tropical Commodities for Quarantine Security, Quality Maintenance, and Value Enhancement. A site visit was made in June, 2007, to meet with Dr. Leon Mu of the Department of Plant Protection, a plant pathologist providing laboratory space and technical assistance in obtaining coffee leaf rust uredospores. Infected coffee was found and leaves with spore bodies were brought to the laboratory. A method of obtaining uredospore germination in water was developed and plans were made for completing the ozone fumigation research in August, 2007. The ADODR monitors progress through regular meetings with cooperators, and through direct supervision of technical staff conducting experiments.