2005 Annual Report 4d.Progress report. This report serves to document research conducted under a specific cooperative agreement between ARS and the International Atomic Energy Agency. Additional details of research can be found in the report for the parent CRIS 5352-22000-017-00D Management of Insect Pests of Temperate Tree Fruit Crops. We have improved the process of screening for transformants and identifying the chromosome location of the transgene, along with basic studies on codling moth cytogenetics. The real-time PCR application has led to the identification of positive parents prior to egg hatch of their offspring. A visiting scientist sponsored by the IAEA-FAO was trained in egg micro-injection techniques. We have developed six genetic codling moth lines continuing in the laboratory which served as test models for all screening procedures. These lines are in the ninth generation (G9). We have perfected FISH (fluorescent in situ hybridization) for determining the location of the transgene in the chromosome. Lastly, we have produced a BAC library of codling moth. We are using this library for mapping and identification of sex specific promoters and enhancers. We are making the library available to interested researchers.