2007 Annual Report 1a.Objectives (from AD-416) Develop environmentally acceptable and economically feasible systems for detection, control, suppression, and eradication of the Mediterranean, oriental, melon, and Malaysian fruit flies and other insect pests associated with tropical fruits, vegetables, and ornamentals. 1b.Approach (from AD-416) Test more environmentally acceptable pesticides and compounds for use in control or eradication programs for fruit flies. Investigate biology and behavior of fruit flies and their parasitoids. Evaluate augmentative parasite releases, mass trapping, male annihilation, sterile fly releases, novel insecticides and food bait sprays as control, suppression, or eradication technologies. Develop new or improved lures, attractants, parapheromones, and baits to be used in improved trap detection devices and/or control systems. Investigate fruit fly and parasitoid olfactory, gustatory, and oviposition aensilla and their electrophysiology. Investigate chemical structure and activity relationships of semiochemicals of fruit flies and their biocontrol agents. Formerly 5320-22430-020-00D (3/05). 4.Accomplishments In spite of being used in California for the last 25 years and for many eradication programs throughout the Pacific, male annihilation with methyl eugenol and cue-lure are still not legally available in Hawaii for control purposes except on an experimental basis. Through research efforts by scientists at the Tropical Plant Pests Research Unit, Pacific Basin Agricultural Research Center, the University of Hawaii, the Hawaii Department of Agriculture and the Hawaii Fruit Fly Area-Wide Pest Management (AWPM) Program, manufacturer's use permits for methyl eugenol and cue-lure were obtained through partnerships with industry. In addition, the first conditional end product registration for Amulet cue-lure fipronil bait stations has been obtained through the U.S. Environmental Protection Agency and three additional end product registrations are pending. The impact of these registrations will be to provide tools for area-wide control of fruit flies when ARS funding for the AWPM program ends in FY08. The AWPM program has been adopted by 2,540 cooperators, on over 607 farms encompassing 6,383 hectares throughout the state of Hawaii. The program has been the recipient of seven national awards for excellence in technology transfer. The accomplishment will directly address NP304, component IV- Problem Area G -Quarantine by improving control technologies for melon fly and oriental fruit fly. 6.Technology Transfer Number of new CRADAs and MTAs 1 Number of active CRADAs and MTAs 1 Number of invention disclosures submitted 1 Number of non-peer reviewed presentations and proceedings 13 Number of newspaper articles and other presentations for non-science audiences 10 Review Publications Jang, E.B., Siderhurst, M.S. 2007. Female biased attraction of oriental fruit fly, bactrocera dorsalis (hendel), to a blend of host fruit volatiles from terminalia catappa l.. Journal of Chemical Ecology. 32:2513-2524 Uchida, G.K., Mcinnis, D.O., Vargas, R.I., Jang, E.B. 2003. Nontarget Anthropods Captured in Melon Fly Monitoring Bucket Traps in Area-wide IPM Projects in Kamuela and Kula in the Hawaiian Islands. Hawaiian Entomological Society Proceedings. 36:135-143 Siderhurst, M.S., Jang, E.B. 2006. Attraction of oriental fruit fly, bactrocera dorsalis to terminalia catappa fruit extracts in wind tunnel and olfactory tests. Formosan Entomologist. 26:45-55. Pinero, J.C., Jacome, I., Vargas, R.I., Prokopy, R.J. 2006. Behavioral response of female melon fly, Bactrocera cucurbitae to host-associated visual and olfactory stimuli. Entomologia Experimentalis et Applicata. 121:261-269. Vargas, R.I., Leblanc, L., Putoa, R., Eitam, A. 2007. Impact of Introduction of Bactrocera dorsalis (Diptera: Tephritidae) and Classical Biological Control Releases, Fopius arisanus (Hymenoptera: Braconidae), on Economically Important Fruit Flies in French Polynesia. Journal of Economic Entomology. 100: 670-679 Khrimian, A., Jang, E.B., Nagata, J.T., Carvalho, L.A. 2006. Consumption and metabolism of 1,2-dimethoxy-4-(3-fluoro-2-propenyl) benzene, a fluorine analog of methyl eugenol, in the oriental fruit fly, bactrocera dorsalis (hendel). Journal of Chemical Ecology. 32:1513-1526 Vargas, R.I., Stark, J., Mackey, B.E., Bull, R. 2005. Weathering trials of amulet(tm) c-l and amulet(tm) m-e attract and kill stations with male melon flies and oriental fruit flies (Diptera: Tephritidae) in Hawaii. Journal of Economic Entomology. 98(5): 1551-1559. Barry, J.D., Miller, N.W., Pinero, J.C., Tuttle, A., Mau, R.F., Vargas, R.I. 2006. Effectiveness of protein baits on melon fly and oriental fruit fly (Diptera: Tephritidae): attraction, feeding, and foraging. Journal of Economic Entomology. 99: 1161-1167.