2005 Annual Report 4d.Progress report. This report serves to document research conducted under a nonfunded cooperative agreement between ARS and Florida A&M University. Additional details of research can be found in the report for the parent project 6618-22000-031-00D Glassy-winged Sharpshooter and Pierce’s Disease. Research conducted under this agreement meets the objectives of the parent project by expanding our understanding of leafhopper/grape interactions. Genetic markers linked to disease and insect resistance were developed for use in a grape breeding program. Production of cDNA libraries from Vitis shuttleworthii grape, resulted in the identification and production of 420 microsatellites and a total of 800 putative genetic markers which may be used to detect disease tolerant and susceptible grape varieties. These genetic markers are currently being screened on grape populations of seedlings to facilitate the rapid screening of thousands of grape plants to produce new disease and insect resistant grape varieties. Prescreening of grape seedlings saves time and thousands of dollars by reducing the number of grape plants needing to be planted into the vineyard for further evaluations.